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1.
Clin Exp Allergy ; 45(7): 1201-13, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25823600

ABSTRACT

BACKGROUND: Only limited evidence is available regarding the cytokine repertoire of effector T cells associated with peanut allergy, and how these responses relate to IgE antibodies to peanut components. OBJECTIVE: To interrogate T cell effector cytokine populations induced by Ara h 1 and Ara h 2 among peanut allergic (PA) children in the context of IgE and to evaluate their modulation during oral immunotherapy (OIT). METHODS: Peanut-reactive effector T cells were analysed in conjunction with specific IgE profiles in PA children using intracellular staining and multiplex assay. Cytokine-expressing T cell subpopulations were visualized using SPICE. RESULTS: Ara h 2 dominated the antibody response to peanut as judged by prevalence and quantity among a cohort of children with IgE to peanut. High IgE (> 15 kU(A)/L) was almost exclusively associated with dual sensitization to Ara h 1 and Ara h 2 and was age independent. Among PA children, IL-4-biased responses to both major allergens were induced, regardless of whether IgE antibodies to Ara h 1 were present. Among subjects receiving OIT in whom high IgE was maintained, Th2 reactivity to peanut components persisted despite clinical desensitization and modulation of allergen-specific immune parameters including augmented specific IgG4 antibodies, Th1 skewing and enhanced IL-10. The complexity of cytokine-positive subpopulations within peanut-reactive IL-4(+) and IFN-γ(+) T cells was similar to that observed in those who received no OIT, but was modified with extended therapy. Nonetheless, high Foxp3 expression was a distinguishing feature of peanut-reactive IL-4(+) T cells irrespective of OIT, and a correlate of their ability to secrete type 2 cytokines. CONCLUSION: Although total numbers of peanut-reactive IL-4(+) and IFN-γ(+) T cells are modulated by OIT in highly allergic children, complex T cell populations with pathogenic potential persist in the presence of recognized immune markers of successful immunotherapy.


Subject(s)
Cytokines/biosynthesis , Peanut Hypersensitivity/immunology , Peanut Hypersensitivity/metabolism , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Th2 Cells/immunology , Th2 Cells/metabolism , 2S Albumins, Plant/immunology , Administration, Oral , Adolescent , Allergens/administration & dosage , Allergens/immunology , Antigens, Plant/administration & dosage , Antigens, Plant/immunology , Child , Child, Preschool , Desensitization, Immunologic , Female , Glycoproteins/immunology , Humans , Immunoglobulin E/blood , Immunoglobulin E/immunology , Immunoglobulin G/blood , Immunoglobulin G/immunology , Immunophenotyping , Infant , Interleukin-4/biosynthesis , Male , Peanut Hypersensitivity/therapy
2.
Allergy ; 57(1): 56-7, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11991295
3.
Am J Physiol Endocrinol Metab ; 280(2): E372-7, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11158943

ABSTRACT

We tested the hypothesis that leptin, in addition to reducing body fat by restraining food intake, reduces body fat through a peripheral mechanism requiring uncoupling protein 1 (UCP1). Leptin was administered to wild-type (WT) mice and mice with a targeted disruption of the UCP1 gene (UCP1 deficient), while vehicle-injected control animals of each genotype were pair-fed to each leptin-treated group. Leptin reduced the size of white adipose tissue (WAT) depots in WT mice but not in UCP1-deficient animals. This was accompanied by a threefold increase in the amount of UCP1 protein and mRNA in the brown adipose tissue (BAT) of WT mice. Leptin also increased UCP2 mRNA in WAT of both WT and UCP1-deficient mice but increased UCP2 and UCP3 mRNA only in BAT from UCP1-deficient mice. These results indicate that leptin reduces WAT through a peripheral mechanism requiring the presence of UCP1, with little or no involvement of UCP2 or UCP3.


Subject(s)
Adipose Tissue, Brown/physiology , Adipose Tissue/anatomy & histology , Adipose Tissue/drug effects , Carrier Proteins/physiology , Leptin/pharmacology , Membrane Proteins/physiology , Membrane Transport Proteins , Mitochondrial Proteins , Adipose Tissue/metabolism , Adipose Tissue, Brown/metabolism , Animals , Body Weight/drug effects , Carrier Proteins/genetics , Carrier Proteins/metabolism , Eating/drug effects , Epididymis , Ion Channels , Male , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mice , Organ Size/drug effects , Proteins/genetics , RNA, Messenger/metabolism , Uncoupling Protein 1 , Uncoupling Protein 2 , Uncoupling Protein 3
4.
J Biol Chem ; 275(42): 33059-67, 2000 Oct 20.
Article in English | MEDLINE | ID: mdl-10938091

ABSTRACT

The three known subtypes of beta-adrenoreceptors (beta(1)-AR, beta(2)-AR, and beta(3)-AR) are differentially expressed in brown and white adipose tissue and mediate peripheral responses to central modulation of sympathetic outflow by leptin. To assess the relative roles of the beta-AR subtypes in mediating leptin's effects on adipocyte gene expression, mice with a targeted disruption of the beta(3)-adrenoreceptor gene (beta(3)-AR KO) were treated with vehicle or the beta(1)/beta(2)-AR selective antagonist, propranolol (20 microgram/g body weight/day) prior to intracerebroventricular (ICV) injections of leptin (0.1 microgram/g body weight/day). Leptin produced a 3-fold increase in UCP1 mRNA in brown adipose tissue of wild type (FVB/NJ) and beta(3)-AR KO mice. The response was unaltered by propranolol in wild type mice, but was completely blocked by this antagonist in beta(3)-AR KO mice. In contrast, ICV leptin had no effect on leptin mRNA in either epididymal or retroperitoneal white adipose tissue (WAT) from beta(3)-AR KOs. Moreover, propranolol did not block the ability of exogenous leptin to reduce leptin mRNA in either WAT depot site of wild type mice. These results demonstrate that the beta(3)-AR is required for leptin-mediated regulation of ob mRNA expression in WAT, but is interchangeable with the beta(1)/beta(2)-ARs in mediating leptin's effect on UCP1 mRNA expression in brown adipose tissue.


Subject(s)
Adipose Tissue, Brown/metabolism , Adipose Tissue/metabolism , Carrier Proteins/genetics , Cerebral Ventricles/physiology , Gene Expression Regulation/physiology , Leptin/genetics , Leptin/pharmacology , Membrane Proteins/genetics , Propranolol/pharmacology , Receptors, Adrenergic, beta-3/physiology , Transcription, Genetic , Adrenergic beta-Antagonists/pharmacology , Animals , Cell Membrane/drug effects , Cell Membrane/physiology , Cerebral Ventricles/drug effects , Imidazoles/pharmacology , Injections, Intraventricular , Ion Channels , Leptin/administration & dosage , Male , Mice , Mice, Inbred C57BL , Mice, Inbred Strains , Mice, Knockout , Mitochondrial Proteins , Propanolamines/pharmacology , RNA, Messenger/genetics , Receptors, Adrenergic, beta-3/deficiency , Receptors, Adrenergic, beta-3/genetics , Receptors, Leptin , Uncoupling Protein 1
5.
Am J Physiol Endocrinol Metab ; 279(2): E356-65, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10913036

ABSTRACT

Obesity-resistant (A/J) and obesity-prone (C57BL/6J) mice were weaned onto low-fat (LF) or high-fat (HF) diets and studied after 2, 10, and 16 wk. Despite consuming the same amount of food, A/J mice on the HF diet deposited less carcass lipid and gained less weight than C57BL/6J mice over the course of the study. Leptin mRNA was increased in white adipose tissue (WAT) in both strains on the HF diet but to significantly higher levels in A/J compared with C57BL/6J mice. Uncoupling protein 1 (UCP1) and UCP2 mRNA were induced by the HF diet in brown adipose tissue (BAT) and WAT of A/J mice, respectively, but not in C57BL/6J mice. UCP1 mRNA was also significantly higher in retroperitoneal WAT of A/J compared with C57BL/6J mice. The ability of A/J mice to resist diet-induced obesity is associated with a strain-specific increase in leptin, UCP1, and UCP2 expression in adipose tissue. The findings indicate that the HF diet does not compromise leptin-dependent regulation of adipocyte gene expression in A/J mice and suggest that maintenance of leptin responsiveness confers resistance to diet-induced obesity.


Subject(s)
Dietary Fats , Leptin/biosynthesis , Membrane Transport Proteins , Mitochondrial Proteins , Obesity/metabolism , Adipose Tissue/metabolism , Adipose Tissue, Brown/metabolism , Animals , Body Weight/physiology , Carrier Proteins/genetics , Carrier Proteins/metabolism , Eating/physiology , Energy Intake/physiology , Gene Expression , Growth/physiology , Ion Channels , Leptin/genetics , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mice , Mice, Inbred A , Mice, Inbred C57BL , Obesity/genetics , Proteins/genetics , Proteins/metabolism , RNA, Messenger/metabolism , Species Specificity , Uncoupling Protein 1 , Uncoupling Protein 2 , Uncoupling Protein 3
6.
Endocrinology ; 140(10): 4772-8, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10499537

ABSTRACT

Exogenous leptin enhances energy utilization in ob/ob mice by binding its hypothalamic receptor and selectively increasing peripheral fat oxidation. Leptin also increases uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT), but the neurotransmitter that mediates this effect has not been established. The present experiments sought to determine whether leptin regulates UCP1 expression in BAT and its own expression in white adipose tissue (WAT) through the long or short forms of leptin receptor and modulation of norepinephrine release. Mice lacking dopamine beta-hydroxylase (Dbh-/-), the enzyme responsible for synthesizing norepinephrine and epinephrine from dopamine, were treated with leptin (20 microg/g body weight/day) for 3 days before they were euthanized. UCP1 messenger RNA (mRNA) and protein expression were 5-fold higher in BAT from control (Dbh+/-) compared with Dbh-/- mice. Leptin produced a 4-fold increase in UCP1 mRNA levels in Dbh+/- mice but had no effect on UCP1 expression in Dbh-/-. The beta3-adrenergic agonist, CL-316,243 increased UCP1 expression and established that BAT from both groups of mice was capable of responding to beta-adrenergic stimulation. Similarly, exogenous leptin reduced leptin mRNA in WAT from Dbh+/- but not Dbh-/- mice. In separate experiments, leptin produced comparable reductions in food intake in both Dbh+/- and Dbh-/- mice, illustrating that norepinephrine is not required for leptin's effect on food intake. Lastly, db/db mice lacking the long form of the leptin receptor failed to increase UCP1 mRNA in response to exogenous leptin but increased UCP1 mRNA in response to CL-316,243. These studies establish that norepinephrine is required for leptin to regulate its own expression in WAT and UCP1 expression in BAT and indicate that these effects are likely mediated through the centrally expressed long form of the leptin receptor.


Subject(s)
Adipose Tissue, Brown/physiology , Adipose Tissue/physiology , Carrier Proteins/metabolism , Gene Expression/physiology , Membrane Proteins/metabolism , Norepinephrine/physiology , Proteins/physiology , Animals , Body Weight/drug effects , Diabetes Mellitus/genetics , Diabetes Mellitus/metabolism , Dopamine beta-Hydroxylase/genetics , Eating/drug effects , Eating/physiology , Ion Channels , Leptin , Male , Mice , Mice, Inbred C57BL , Mice, Knockout/genetics , Mice, Knockout/metabolism , Mitochondrial Proteins , Proteins/genetics , Proteins/pharmacology , RNA, Messenger/metabolism , Uncoupling Protein 1
7.
Endocrinology ; 140(1): 292-300, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9886838

ABSTRACT

Deposition of excess body fat occurs when energy intake chronically exceeds energy expenditure. In ob/ob mice, the absence of leptin affects both components of the energy balance equation, and the mice become morbidly obese after weaning. Treatment of ob/ob mice with exogenous leptin reduces body weight by decreasing food intake and stimulating energy utilization, but even when saline- and leptin-injected ob/ob mice are pair-fed, mice receiving leptin lose significantly more weight. Therefore, the purpose of the present study was to test the hypotheses that uncoupling protein-1 (UCP1) expression is reduced in adipose tissue from ob/ob mice and is restored by treatment with exogenous leptin. Lean and ob/ob mice (5-6 weeks old) were housed at 23 C and treated with leptin (20 microg/g BW x day) for 3 days before they were killed. Compared with levels in lean littermates, UCP1 messenger RNA (mRNA) and protein levels were lower in brown adipose tissue (BAT) and retroperitoneal white adipose tissue (WAT) from ob/ob mice. Treatment of ob/ob mice with leptin reduced body weight and produced a 4- to 5-fold increase in UCP1 mRNA levels in both interscapular BAT and retroperitoneal WAT. The increases in UCP1 mRNA were accompanied by comparable increases in UCP1 protein in mitochondrial preparations from each tissue. Given that the sole known function of UCP1 is to uncouple oxidative phosphorylation, the present results are consistent with the conclusion that leptin stimulates energy utilization in ob/ob mice by increasing thermogenic activity and capacity (UCP1). In addition, the present results suggest that decreased UCP1 expression in BAT and WAT of ob/ob mice is in part responsible for their increased metabolic efficiency and propensity to become obese.


Subject(s)
Adipose Tissue, Brown/metabolism , Adipose Tissue/metabolism , Carrier Proteins/biosynthesis , Membrane Proteins/biosynthesis , Membrane Transport Proteins , Mitochondrial Proteins , Protein Biosynthesis , Proteins/pharmacology , Adipose Tissue/drug effects , Adipose Tissue, Brown/drug effects , Animals , Blotting, Western , Body Weight/drug effects , Energy Metabolism/drug effects , Epididymis , Ion Channels , Leptin , Male , Mice , Mice, Inbred C57BL , Mitochondria/drug effects , Mitochondria/metabolism , Obesity/metabolism , Peritoneum , RNA, Messenger/metabolism , Uncoupling Protein 1 , Uncoupling Protein 2
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