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1.
RSC Adv ; 11(5): 3134-3142, 2021 Jan 11.
Article in English | MEDLINE | ID: mdl-35424261

ABSTRACT

Isothiocyanates (ITCs) are typically prepared using amines and highly toxic reagents such as thiophosgene, its derivatives, or CS2. In this work, an investigation of a multicomponent reaction (MCR) using isocyanides, elemental sulfur and amines revealed that isocyanides can be converted to isothiocyanates using sulfur and catalytic amounts of amine bases, especially DBU (down to 2 mol%). This new catalytic reaction was optimized in terms of sustainability, especially considering benign solvents such as Cyrene™ or γ-butyrolactone (GBL) under moderate heating (40 °C). Purification by column chromatography was further optimized to generate less waste by maintaining high purity of the product. Thus, E-factors as low as 0.989 were achieved and the versatility of this straightforward procedure was shown by converting 20 different isocyanides under catalytic conditions, while obtaining moderate to high yields (34-95%).

2.
Gastroenterology ; 82(1): 71-6, 1982 Jan.
Article in English | MEDLINE | ID: mdl-6273252

ABSTRACT

In this paper we describe 2 patients with herpes simplex hepatitis. Submassive liver necrosis occurred in both patients, one of whom survived. Notable in the clinical course of both patients was the almost simultaneous occurrence of three events-fever, marked elevation of serum transaminases, and leukopenia. Using an immunoperoxidase staining technique we demonstrated herpes simplex viral antigen in the nucleus, cytoplasm, and cell membrane of affected hepatocytes.


Subject(s)
Antigens, Viral/analysis , Hepatitis, Viral, Human/etiology , Herpes Simplex/diagnosis , Simplexvirus/immunology , Adult , Aged , Alanine Transaminase/blood , Aspartate Aminotransferases/blood , Female , Fever , Hepatitis, Viral, Human/diagnosis , Humans , Immunoenzyme Techniques , Leukopenia/diagnosis , Male
5.
Cell Tissue Res ; 158(4): 425-37, 1975 May 20.
Article in English | MEDLINE | ID: mdl-1164721

ABSTRACT

This study describes distrinctive cells with ultrastructural and histochemical features of APUD-type endocrine cells within the tracheal epithelium of human fetuses, newborns and children as well as different animal species. These cells referred to as Kultschitzky cells (K cells) were found to be argyrophilic, but not argentaffin, and are considered analogous to the same type of cells in lung and gastro-intestinal tract. Fluorescence histochemistry demonstrated the presence of intracellular amine within tracheal K cells, but only after in-vitro or in-vivo administration of amine precursor (L-DOPA). Ultrastructurally, these cells are characterized by the presence of numerous cytoplasmic granules (dense core vesicles) which show species related morphologic variations. Two different types of K cells were found in trachea of lamb and armadillo, each type possessing morphologically different dense core vesicles. In human and rabbit tracheas, only one type of K cell was identified. K cells in the trachea are distributed as single cells between other epithelial cells; neuroepithelial bodies such as those found in bronchial mucosa were not identified. Well differentiated K cells were found in tracheas of early human fetuses and throughout gestation, infancy, and childhood. Preservation of K cells in human autopsy material and widespread occurence of these cells in various laboratory animals will permit further studies into the nature and function of tracheobronchial endocrine cells.


Subject(s)
Chromaffin System/ultrastructure , Trachea/ultrastructure , Animals , Armadillos , Cell Differentiation , Epithelial Cells , Epithelium/ultrastructure , Female , Histocytochemistry , Humans , In Vitro Techniques , Mucous Membrane/ultrastructure , Pregnancy , Rabbits , Sheep , Species Specificity
6.
Can Med Assoc J ; 110(10): 1151-4, 1974 May 18.
Article in English | MEDLINE | ID: mdl-4524533

ABSTRACT

Two cases of chronic myeloid leukemia in which the cytogenetic clone was 45, XO, Ph(1) are described and compared with 20 cases recorded in the literature. The 45, XO line is peculiar to the leukemic cells and is not a manifestation of constitutional mosaicism. It probably arises from a 46, XY, Ph(1) line by loss of the Y chromosome. Because of the few cases reported, one cannot be certain that these men have a better prognosis, although the median survival time suggests that this is so. Infertility is not part of this disorder.


Subject(s)
Chromosome Aberrations , Chromosomes, Human, 21-22 and Y , Leukemia, Myeloid/genetics , Sex Chromosomes , Adult , Humans , Karyotyping , Leukemia, Myeloid/mortality , Male , Middle Aged , Sex Factors
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