miR-497 inhibits tumor growth and migration of osteosarcoma by targeting plexinA4 and CDK6.

MicroRNAs are small non-coding RNAs that regulate gene expression at the post-transcriptional level which have been reported to be involved in the pathogenesis of various cancers. In the present study, we found that miR-497 was downregulated in osteosarcoma tissues. Gain and loss of function studies were carried out to investigate the effect of miR-497 on the growth of osteosarcoma cells. The results indicated that miR-497 inhibited the growth of osteosarcoma cells. Furthermore, bioinformatics analysis predicted plexinA4 and CDK6 as targets of miR-497, which was afterward confirmed by luciferase activity assay and rescue experiments. These findings suggested that miR-497, plexinA4 and CDK6 may serve as novel potential makers for osteosarcoma diagnostics and therapy.

[Functional Characterization of ZmHAK1 Promoter and Its Regulatory Transcription Factors in Maize].

Potassium (K^(+)) deficiency in the soil may seriously affect the yield and quality of plants, which usually satisfy their potassium requirements by engaging their K^(+) transporters and/or channels. High-affinity potassium transporter (ZmHAK) family members play crucial role in the uptake and distribution of K^(+) in maize (Zea mays L.). Here, we describe the function of ZmHAK1 promoter and its upstream regulatory transcription factors in maize. In this plant, HAK gene family includes 34 protein-encoding members, with their phylogenetic tree analysis showing both evolutionary conservativeness and diversity. ZmHAK1 gene promoter contains many functional elements related to abiotic stress. Reporter construct pCambia1301:ProZmHAK1:GUS shows that the ZmHAK1 gene is active in the roots, stems, and leaves. Using yeast one-hybrid experiment, we showed that the ZmHAK1 promoter interacts with the transcription factors ZmRAP2.11 and ZmARF2, and that these interactions occur on different fragments of the ZmHAK1 promoter. Transcription factor ZmRAP2.11 localizes in the nucleus, while ZmARF2 is found both in the nucleus and in the cell cytoplasm. In conclusion, our results suggest that the ZmHAK1 regulation has an important role in the process of absorbing potassium ions, and possibly in the response of maize to abiotic stress.

Aß1-42 regulates astrocytes through JNK/AP-1 pathway.

OBJECTIVE: In Alzheimer's disease (AD), astrocytes are generally found in the surrounding of senile plaques participating in the production of phagocytosis and the removal of toxic compounds such as Aß. This study aimed at investigating the effect of Aß1-42 on astrocytes. MATERIALS AND METHODS: Cellular viability of primary cultured astrocytes was analyzed using CCK-8 assay. Quantitative Real-time PCR was used to assess the mRNA expression of JNK and AP-1. The proteins of JNK/AP-1 pathway were investigated using Western blot. RESULTS: Our findings showed that Aß1-42 inhibited cell viability and promoted apoptosis in astrocytes in primary culture. Additionally, Aß1-42 increased the mRNA expression level of AP-1, but had no effect on the expression of JNK. Furthermore, Aß1-42 increased the protein expression of p-JNK, p-c-jun and Fra-1 and the ratio of p-c-jun/c-jun and p-JNK/JNK. CONCLUSIONS: We showed that Aß1-42 promoted cell apoptosis in astrocytes in primary culture. Furthermore, Aß1-42 activated JNK/AP-1 pathway through promoting the phosphorylation of JNK, c-jun and Fra-1 expression, then inducing cell apoptosis.

Recent advances in massage therapy--a review.

Massage therapy is one of the most widely accepted alternative form of medicine helping patients suffering from varied pathological states including arthritis, anxiety, sleep problems, pain management and injury repair. Besides this, it is one of the safest forms of alternative medicine and has become favorite among various health care professionals. However, there is still a lot of debate is going in medical world pertaining to its certain use in modern medicine. So, the present review shall enlighten all the latest aspects of massage therapy in current medicine.

Thermomechanical properties of Ni-Ti shape memory wires containing nanoscale precipitates induced by stress-assisted ageing.

This paper systematically examines the thermomechanical properties and phase transformation behaviour of slightly Ni-rich Ni-Ti biomedical shape memory wires containing homogeneously distributed nanoscale precipitates induced by stress-assisted ageing. In contrast to previous studies, particular attention is paid to the role of precipitates in impeding twin boundary movement (TBM) and its underlying mechanisms. The size and volume fraction of precipitates are altered by changing the ageing time. The martensitic transformation temperatures increase with prolonged ageing time, whereas the R-phase transformation temperature remains relatively unchanged. The stress-strain behaviour in different phase regions during both cooling and heating is comprehensively examined, and the underlying mechanisms for the temperature- and thermal-history-dependent behaviour are elucidated with the help of the established stress-temperature phase diagram. The effect of precipitates on TBM is explored by mechanical testing at 133K. It is revealed that the critical stress for TBM (σcr) increases with increasing ageing time. There is a considerable increase of 104MPa in σcr in the sample aged at 773K for 120min under 70MPa compared with the solution-treated sample, owing to the presence of precipitates. The Orowan strengthening model of twinning dislocations is insufficient to account for this increase in σcr. The back stress generation is the predominant mechanism for the interactions between precipitates and twin boundaries during TBM that give rise to the increase in σcr. Such results provide new insights into the thermomechanical properties of precipitate containing Ni-Ti biomedical shape memory wires, which are instructive for developing high-performance biomedical shape memory alloys.

A linearly convergent first-order algorithm for total variation minimisation in image processing.

We introduce a new formulation for total variation minimisation in image denoising. We also present a linearly convergent first-order method for solving this reformulated problem and show that it possesses a nearly dimension-independent iteration complexity bound.

Spontaneous eye blinks are entrained by finger tapping.

We studied the mutual cross-talk between spontaneous eye blinks and continuous, self-paced unimanual and bimanual tapping. Both types of motor activities were analyzed with regard to their time-structure in synchronization-continuation tapping tasks which involved different task instructions, namely "standard" finger tapping (Experiment 1), "strong" tapping (Experiment 2) requiring more forceful finger movements, and "impulse-like" tapping (Experiment 3) where upward-downward finger movements had to be very fast. In a further control condition (Experiment 4), tapping was omitted altogether. The results revealed a prominent entrainment of spontaneous blink behavior by the manual tapping, with bimanual tapping being more effective than unimanual tapping, and with the "strong" and "impulse-like" tapping showing the largest effects on blink timing. Conversely, we found no significant effects of the tapping on the timing of the eye blinks across all experiments. The findings suggest a functional overlap of the motor control structures responsible for voluntary, rhythmic finger movements and eye blinking behavior.

Absorption of benzoic acid in segmental regions of the vascularly perfused rat small intestine preparation.

Oral bioavailability is a consequence of intestinal absorption, exsorption, and metabolism and is further modulated by the difference in activities among segmental regions. The influence of these factors on the net absorption of benzoic acid (BA), a substrate that is metabolized to hippurate and is transported by the monocarboxylic acid transporter 1, was studied in the recirculating, vascularly perfused, rat small intestine preparation. Metabolism of BA was not observed for both systemic and intraluminal injections into segments of varying lengths. But, secretion of BA into lumen was noted. Absorption of BA (0.166-3.68 micromol) introduced at the duodenal end for absorption by the entire intestine was complete (>95% dose at 2 h) and dose-independent, yielding similar absorption rate constants (k(a) of 0.0464 min(-1)). The extent of absorption remained high (92-96% dose) when BA was injected into closed segments of shorter lengths (12 or 20 cm), suggesting a large reserve length of the rat intestine. However, k(a) was higher for the jejunum (0.0519 and 0.0564 min(-1), respectively, for the 12- and 20-cm segments) and exceeded that for the duodenum (12-cm segment, 0.0442 min(-1)) and ileum (20-cm segment, 0.0380 min(-1)) at closed injection sites. The finding paralleled the distribution of monocarboxylic acid transporter isoform 1 detected by Western blotting along the length of the small intestine. Fits of the systemic and oral data (based on duodenal injection for absorption by the whole intestine) to the traditional, physiological model and to the segregated flow model (SFM) that describes partial intestinal flow to the enterocyte region showed a better fit with the SFM even though metabolite data were absent.

[Health indicators among students from Hô Chi Minh-Ville (Vietnam): comparison with other countries]. / Les représentations de la santé chez les lycéens à Hô Chi Minh-Ville (Vietnam): comparaison avec d'autres pays.

In Vietnam, as in most developing countries, adolescent health is not considered a priority, yet this population faces many health problems. This study aims to describe perceptions of health among high school students in Ho Chi Ming City, Vietnam. The study was carried out with a representative sample of 543 students between the ages of 16 and 19, using a tool adapted from a questionnaire that was created and validated by the Centre for Preventive Medicine in Nancy, France. The adolescents who participated in the study described their perceptions of health in diverse ways: on the one hand they described them in a classic manner (absence of sickness) and on the other hand they described them in a more comprehensive, positive way (fitness, strength, development, happiness). A comparison with other studies in Algeria and France that used the same questionnaire shows certain similarities across cultures with regard to perceptions of health, as well as certain characteristics that are particular to each society and each culture.

[Pollen morphology of bee plants in Changbai Mountain area].

OBJECTIVE: To develop and utilize the bee plants and bee products in Changbai Mountain area, the representative area of alpine plants in northeast China featuring abundant resources of bee plants. METHOD: The pollen of bee plants was observed and studied by LM and SEM. RESULT: The pollen is morphologically oblate, suboblate, spheroidal and prolate. The external sculpture is commonly reticulate, striate and echinulate. CONCLUSION: Plant pollen identification criteria have been furnished for the development of bee plant resources as well as the inspection of bee products in Changbai Mountain area.

A new physiologically based, segregated-flow model to explain route-dependent intestinal metabolism.

Processes of intestinal absorption, metabolism, and secretion must be considered simultaneously in viewing oral drug bioavailability. Existing models often fail to predict route-dependent intestinal metabolism, namely, little metabolism occurs after systemic dosing but notable metabolism exists after oral dosing. A physiologically based, Segregated-Flow Model (SFM) was developed to examine the influence of intestinal transport (absorption and exsorption), metabolism, flow, tissue-partitioning characteristics, and elimination in other organs on intestinal clearance, intestinal availability, and systemic bioavailability. For the SFM, blood flow to intestine was effectively segregated for the perfusion of two regions, with 10% reaching an absorptive layer-the enterocytes at the villus tips of the mucosa where metabolic enzymes and the P-glycoprotein reside, and the remaining 90% supplying the rest of the intestine (serosa and submucosa), a nonabsorptive layer. The traditional, physiologically-based model, which regards the intestine as a single, homogeneous compartment with all of the intestinal blood flow perfusing the tissue, was also examined for comparison. The analytical solutions under first order conditions were essentially identical for the SFM and traditional model, differing only in the flow rate to the absorptive/removal region. The presence of other elimination organs did not affect the intestinal clearance and bioavailability estimates, but reduced the percentage of dose metabolized by the intestine. For both models, intestinal availability was inversely related to the intrinsic clearances for intestinal metabolism and exsorption, and was additionally affected by both the rate constant for absorption and that denoting luminal loss when drug was exsorbed. However, the effect of secretion by P-glycoprotein became attenuated with rapid absorption. The difference in flow between models imparted a substantial influence on the intestinal clearance of flow-limited substrates, and the SFM predicted markedly higher extents of intestinal metabolism for oral over i.v. dosing. Thus, the SFM provides a physiological view of the intestine and explains the observation of route-dependent, intestinal metabolism.