ABSTRACT
Nature-derived bioactive compounds have emerged as promising candidates for the prevention and treatment of diverse chronic illnesses, including neurodegenerative diseases. However, the exact molecular mechanisms underlying their neuroprotective effects remain unclear. Most studies focus solely on the antioxidant activities of natural products which translate to poor outcome in clinical trials. Current therapies against neurodegeneration only provide symptomatic relief, thereby underscoring the need for novel strategies to combat disease onset and progression. We have employed an environmental toxin-induced Drosophila Parkinson's disease (PD) model as an inexpensive in vivo screening platform to explore the neuroprotective potential of selected dietary flavonoids. We have identified a specific group of flavonoids known as flavones displaying protection against paraquat (PQ)-induced neurodegenerative phenotypes involving reduced survival, mobility defects, and enhanced oxidative stress. Interestingly, the other groups of investigated flavonoids, namely, the flavonones and flavonols failed to provide protection indicating a requirement of specific structural features that confer protection against PQ-mediated neurotoxicity in Drosophila. Based on our screen, the neuroprotective flavones lack a functional group substitution at the C3 and contain α,ß-unsaturated carbonyl group. Furthermore, flavones-mediated neuroprotection is not solely dependent on antioxidant properties through nuclear factor erythroid 2-related factor 2 (Nrf2) but also requires regulation of the immune deficiency (IMD) pathway involving NFκB and the negative regulator poor Imd response upon knock-in (Pirk). Our data have identified specific structural features of selected flavonoids that provide neuroprotection against environmental toxin-induced PD pathogenesis that can be explored for novel therapeutic interventions.
Subject(s)
Flavones , Neuroprotective Agents , Parkinson Disease , Animals , Parkinson Disease/drug therapy , Parkinson Disease/etiology , Parkinson Disease/prevention & control , Flavonoids/pharmacology , Flavonoids/therapeutic use , Drosophila , Antioxidants/pharmacology , Neuroprotection , Oxidative Stress , Flavones/pharmacology , Flavones/therapeutic use , Paraquat/toxicity , Neuroprotective Agents/pharmacology , Neuroprotective Agents/therapeutic useABSTRACT
Vehicle rollovers cause many agricultural work-related fatalities each year. Tractors, off-road utility vehicles (ORUVs), zero turn radius (ZTR) mowers, and all-terrain vehicles (ATVs) can all become involved in fatal rollovers. The rollover tendency of these vehicles was evaluated using static lateral and longitudinal stability angles. Center of gravity locations were measured with the lift axle method, and lateral and longitudinal stability angles were calculated for four ATVs, five ORUVs, four ZTR mowers, and four lawn tractors. Stability angles were calculated for loaded and unloaded vehicle conditions. Loading vehicles with ballast and operators can substantially decrease lateral and longitudinal stability angles. Stability angles for these vehicles and for five full-size agricultural tractors were compared. All loaded and unloaded, lateral and longitudinal stability angles determined met the appropriate ANSI requirements.
