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2.
J Neuroendocrinol ; 25(7): 644-54, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23631684

ABSTRACT

Individuals integrate information about their environment into adaptive behavioural responses, yet how different sensory modalities contribute to these decisions and where in the brain this integration occurs is not well understood. We presented male cichlid fish (Astatotilapia burtoni) with sensory information in three social contexts: intruder challenge, reproductive opportunity and a socially neutral situation. We then measured behavioural and hormonal responses along with induction of the immediate early gene c-Fos in candidate forebrain regions. In the intruder challenge context, males were exposed to either a visual stimulus of a dominant male, the putative male pheromone androstenedione, or both. We found that, compared to the neutral context, a visual stimulus was necessary and sufficient for an aggressive response, whereas both chemical and visual stimuli were needed for an androgen response. In the reproductive opportunity context, males were exposed to either a visual stimulus of a receptive female, a progesterone metabolite (female pheromone) only, or both. We further found that the visual stimulus is necessary and sufficient for an androgen response in the reproductive opportunity context. In the brain, we observed c-Fos induction in response to a visual challenge stimulus specifically in dopaminergic neurones of area Vc (the central region of the ventral telencephalon), a putative striatal homologue, whereas presentation of a chemical stimulus did not induce c-Fos induction in the intruder challenge context. Our results suggest that different sensory cues are processed in a social context-specific manner as part of adaptive decision-making processes.


Subject(s)
Behavior, Animal , Cichlids/physiology , Neuroendocrine Cells/physiology , Social Environment , Animals , Female , Male
3.
Genes Brain Behav ; 11(7): 813-8, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22862958

ABSTRACT

Sex steroid hormones coordinate neurotransmitter systems in the male brain to facilitate sexual behavior. Although neurotransmitter release in the male brain has been well documented, little is known about how androgens orchestrate changes in gene expression of neurotransmitter receptors. We used male whiptail lizards (Cnemidophorus inornatus) to investigate how androgens alter neurotransmitter-related gene expression in brain regions involved in social decision making. We focused on three neurotransmitter systems involved in male-typical sexual behavior, including the N-methyl-d-aspartate (NMDA) glutamate receptor, nitric oxide and dopamine receptors. Here, we show that in androgen-treated males, there are coordinated changes in neurotransmitter-related gene expression. In androgen-implanted castrates compared with blank-implanted castrates (control group), we found associated increases in neuronal nitric oxide synthase gene expression in the nucleus accumbens (NAcc), preoptic area and ventromedial hypothalamus, a decrease of NR1 gene expression (obligate subunit of NMDA receptors) in the medial amygdaloid area and NAcc and a decrease in D1 and D2 dopamine receptor gene expression in the NAcc. Our results support and expand the current model of androgen-mediated gene expression changes of neurotransmitter-related systems that facilitate sexual behavior in males. This also suggests that the proposed evolutionarily ancient reward system that reinforces sexual behavior in amniote vertebrates extends to reptiles.


Subject(s)
Androgens/pharmacology , Gene Expression Regulation/drug effects , Lizards/genetics , Nitric Oxide Synthase/genetics , Receptors, Dopamine/genetics , Receptors, N-Methyl-D-Aspartate/genetics , Animals , Hypothalamus/metabolism , Lizards/metabolism , Male , Nitric Oxide Synthase/metabolism , Orchiectomy , Receptors, Dopamine/metabolism , Receptors, N-Methyl-D-Aspartate/metabolism , Sexual Behavior, Animal/drug effects
4.
Clin Rehabil ; 26(1): 68-80, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21971756

ABSTRACT

OBJECTIVE: To systematically review the psychometric properties and clinical utility of measures of sensation in neurological conditions to inform future research studies and clinical practice. DATA SOURCES: Electronic databases (MEDLINE, CINAHL, EMBASE and AMED) were searched from their inception to December 2010. REVIEW METHODS: Search terms were used to identify articles that investigated any sensory measures in neurological conditions. Data about their psychometric properties and clinical utility were extracted and analyzed independently. The strength of the psychometric properties and clinical utility were assessed following recommendations. (1) RESULTS: Sixteen sensory measures were identified. Inter-rater reliability and redundancy of testing protocols are particular issues for this area of assessment. Eleven were rejected because they were not available for a researcher or clinician to use. Of the remaining five measures, the Erasmus MC modifications of the Nottingham Sensory Assessment and the Sensory section of the Fugl-Meyer Assessment showed the best balance of clinical utility and psychometric properties. CONCLUSION: Many measures of sensory impairment have been used in research but few have been fully developed to produce robust data and be easy to use. At present, the sensory section of the Fugl-Meyer Assessment and the Erasmus MC modifications of the Nottingham Sensory Assessment show the most effective balance of usability and robustness, when delivered according to the operating instructions.


Subject(s)
Diagnostic Techniques, Neurological , Nervous System Diseases/physiopathology , Psychometrics/instrumentation , Sensation/physiology , Somatosensory Disorders/diagnosis , Databases, Bibliographic , Humans , Nervous System Diseases/complications , Reproducibility of Results , Somatosensory Disorders/etiology
5.
J Neuroendocrinol ; 23(3): 244-53, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21126273

ABSTRACT

The evolution of neuroendocrine mechanisms governing sex-typical behaviour is poorly understood. An outstanding animal model is the whiptail lizard (Cnemidophorus) because both the ancestral and descendent species still exist. The ancestral little striped whiptail, Cnemidophorus inornatus, consists of males and females, which exhibit sex-specific mating behaviours. The descendent desert grassland whiptail, Cnemidophorus uniparens, consists only of females that alternately exhibit both female-like and male-like pseudosexual behaviour. Castrated male C. inornatus will mount a conspecific in response to exogenous androgen, although some are also sensitive to progesterone. This polymorphism in progesterone sensitivity in the ancestral species may have been involved in evolution of progesterone-mediated male-typical behaviour in the descendant unisexual lizards. We tested whether progesterone activates a typically androgenic signalling pathway by investigating hormonal regulation of neuronal nitric oxide synthase (nNOS) using in situ hybridisation and NADPH diaphorase histochemistry, a stain for nNOS protein. NADPH diaphorase is widely distributed throughout the brain of both species, although only in the periventricular nucleus of the preoptic area (pvPOA) are there differences between mounting and non-mounting individuals. The number of cells expressing nNOS mRNA and NADPH diaphorase is higher in the pvPOA of individuals that mount in response to progesterone or androgen. Furthermore, the nNOS promoter has both androgen and progesterone response elements, and NADPH diaphorase colocalises with the progesterone receptor in the pvPOA. These data suggest that a polymorphism in progesterone sensitivity in the sexual ancestor reflects a differential regulation of nNOS and may account for the male-typical behaviour in unisexual whiptail lizards.


Subject(s)
Biological Evolution , Lizards/physiology , Nitric Oxide Synthase Type I/genetics , Parthenogenesis/genetics , Sexual Behavior, Animal/physiology , Animals , Cloning, Molecular , Estrous Cycle/genetics , Estrous Cycle/metabolism , Estrous Cycle/physiology , Female , Gender Identity , Lizards/genetics , Lizards/metabolism , Male , Models, Biological , Nitric Oxide Synthase Type I/metabolism , Nitric Oxide Synthase Type I/physiology , Ovary/metabolism , Parthenogenesis/physiology , Receptors, Progesterone/genetics , Receptors, Progesterone/metabolism , Sex Characteristics , Tissue Distribution
6.
Disabil Rehabil ; 31(18): 1494-500, 2009.
Article in English | MEDLINE | ID: mdl-19848560

ABSTRACT

OBJECTIVE: The aim of this study was to identify the treatment packages (combinations of interventions) used to treat postural control and mobility problems for patients with stroke. METHOD: A convenience sample of 74 physiotherapists from 34 National Health Service hospitals recorded the interventions used to treat postural control and mobility problems for 251 patients with stroke in 1156 treatment sessions using the Stroke Physiotherapy Intervention Recording Tool (Tyson and Selley, Disabil Rehabil 2004;26:1184 - 1188). Descriptive statistics assessed the frequency with which the interventions were used and geometric coding identified treatment packages. RESULTS: The most frequently used interventions involved facilitation, practice of activities and their components and mobilisations. The least frequently used interventions involved the provision of equipment, teaching carers or professionals and exercise. Two treatment packages were identified; one involving the facilitation (of activities and their components) and the other involving whole activities (facilitation and practice). CONCLUSIONS: Interventions are often combined in two treatment packages to treat postural control and mobility problems after stroke. One involved facilitation (of whole and component activities) and the other involved practice and facilitation of whole activities. Future research in which conventional or standard UK stroke physiotherapy is delivered should focus on these interventions and exclude atypically used interventions.


Subject(s)
Dependent Ambulation , Physical Therapy Modalities , Postural Balance , Stroke Rehabilitation , Aged , Aged, 80 and over , Allied Health Personnel , Data Collection , Humans , Recovery of Function , United Kingdom
7.
Clin Rehabil ; 23(9): 824-40, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19656816

ABSTRACT

OBJECTIVE: To identify psychometrically robust and clinically feasible measurement tools of balance activity in people with neurological conditions to recommend for use in clinical practice. DATA SOURCES: MEDLINE, CINAHL, EMBASE, PEDro and AMED. REVIEW METHODS: Independent reviewers selected and extracted data from articles that assessed the reliability, validity, sensitivity to change and clinical utility of measures of balance activity in adult neurological conditions. Quality assessment was based on Jorstad et al. Measures with 'good' psychometrics and > or =9/10 clinical utility scores were recommended. RESULTS: Nineteen measurement tools were selected. Of these, the Brunel Balance Assessment, Berg Balance Scale, Trunk Impairment Scale, arm raise and forward reach tests in sitting and standing, weight shift, step/tap and step-up tests reached the required standards and are usable in clinical practice. The Brunel Balance Assessment and its associated functional performance tests have the additional advantages of being a hierarchical scale with established lack of redundancy. CONCLUSION: The measurement tools identified above are psychometrically robust and feasible to use in clinical practice. Future objective measure development should consider the theoretical construct of the measure, the minimal detectable change and use in clinical populations other than stroke.


Subject(s)
Disability Evaluation , Postural Balance , Task Performance and Analysis , Humans , Physical Examination , Psychometrics , Reproducibility of Results , Stroke/complications , Stroke Rehabilitation
8.
Disabil Rehabil ; 31(6): 448-57, 2009.
Article in English | MEDLINE | ID: mdl-18720129

ABSTRACT

OBJECTIVE: The aim of this study was to identify which interventions used to treat postural control and mobility are considered part of the Bobath concept (BC). DESIGN. Hospital-based UK stroke physiotherapists identified interventions which they perceived to be part of the BC from a pre-published list of interventions used to treat postural control and mobility problems. Interventions that > 75% of participants felt were part of the BC were classified as 'definitely Bobath'. Interventions that < 25% felt were part of the BC were classified as 'definitely not Bobath'. Other interventions were classified as 'unsure'; those indentified by 50-74% of participants as part of the BC were classified as 'probably Bobath' and those indentified 26-49% were classified as 'probably not Bobath'. RESULTS: Seventy-four physiotherapists from 33 hospitals participated. Facilitation, mobilizations and practicing components of activities were most strongly associated with the BC. Exercise and the use of equipment were identified as 'not' or 'probably not Bobath'. There was uncertainty about practicing activities, teaching patients and carers and arranging independent practice. CONCLUSIONS: UK stroke physiotherapists perceive that the BC involves interventions that focus on facilitating movement, mobilization, practicing components of activities and some whole activities. Their views about what is not part of the BC and the areas where they are uncertain contrast with British and international teachers of the BC. Consequently, it was not possible to define a 'typical package' of treatment for postural control and mobility that represents the BC. Future research into the BC should focus on the effectiveness of specific, well-defined interventions.


Subject(s)
Locomotion , Physical Therapy Modalities , Physical Therapy Specialty , Posture , Stroke Rehabilitation , Humans , United Kingdom
9.
Clin Rehabil ; 22(8): 758-67, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18678576

ABSTRACT

OBJECTIVE: To investigate the frequency of somatosensory impairment in stroke patients within different somatosensory modalities and different body areas, and their recovery. DESIGN: Prospective observational study. SETTING: Two stroke rehabilitation units. SUBJECTS: Seventy patients with a first stroke (36 men, 34 women; average age, 71, SD 10.00 years; average time since stroke onset, 15 days) were assessed on admission and two, four and six months after stroke. INTERVENTIONS: Not applicable. MAIN MEASURE: Nottingham Sensory Assessment. RESULTS: Somatosensory impairment was common after stroke; 7-53% had impaired tactile sensations, 31-89% impaired stereognosis, and 34-64% impaired proprioception. When comparing somatosensory modalities within body areas the kappa values were low (kappa values<0.54). Recovery occurred over time, though not significantly in lower limb tactile sensations. Stroke severity was the main factor influencing initial somatosensory impairment, but accounted for a small amount of the variance (21-41%). Initial somatosensory impairment was significantly related to somatosensory ability at six months, accounting for 46-71% of the variance. CONCLUSIONS: Proprioception and stereognosis were more frequently impaired than tactile sensations. The different somatosensory modalities showed only slight agreement between impairment within the same body areas, suggesting that the modalities are independent of each other and all should be assessed. High agreements were found between different body areas for each somatosensory modality. Somatosensory impairment was associated with stroke severity, however low variance indicated other factors were involved.


Subject(s)
Recovery of Function , Somatosensory Disorders/rehabilitation , Stroke Rehabilitation , Aged , Aged, 80 and over , Female , Humans , Longitudinal Studies , Lower Extremity , Male , Middle Aged , Somatosensory Disorders/etiology , Stereognosis , Stroke/complications , Touch , Upper Extremity
10.
J Matern Fetal Neonatal Med ; 17(2): 139-43, 2005 Feb.
Article in English | MEDLINE | ID: mdl-16076623

ABSTRACT

BACKGROUND: Audit is important in ensuring adequate use of resources and maintaining optimum standards of care. Most of the emphasis in neonatal audit is focused on very low birth weight infants. However, term and near-term infants account for a significant proportion of the workload in neonatal units and warrant regular audit. In addition, audit of these infants may be useful as a marker of the organisation of the perinatal service. METHODS: A retrospective audit was performed of all infants with birth weights greater than or equal to 2,500 grams admitted to the neonatal department in the first week of life over a two-year period, examining mode of delivery, level of care, duration of stay, diagnosis and short-term outcome. RESULTS: Eight hundred and seventy infants were admitted greater than or equal to 2,500 grams birth weight, 54% of all neonatal admissions, during the study period. Six hundred and eighty seven of these infants were admitted in the first week of life and were included in the study; this was 5.8% of infants born with a birth weight 2500 grams or more. Infants born by caesarean section were twice as likely to require admission (9.8%) compared with infants born by vaginal delivery (4.5%). The median length of stay was 3 days (3 hours to 45 days). One hundred and six (15.4%) infants required level 1 or level 2 care. One hundred and eleven infants received normal care, only. Most of these infants were admitted for maternal or social reasons. Other common reasons for admission were jaundice, respiratory disease, neonatal abstinence syndrome and congenital abnormality. Forty-one infants required transfer to another hospital, most commonly for surgical or cardiac conditions. Six infants died after admission. However, only one normally formed infant delivered in our hospital died prior to discharge or transfer. One infant was born at home and four infants who had a lethal congenital abnormality are known to have died following transfer. CONCLUSION: Term and near-term infants account for a significant proportion of neonatal admissions and deserve regular audit. Many admissions are potentially avoidable. Survival for infants weighing 2,500 grams or greater is excellent. Only one normally formed infant died following admission during the study period. The number of "social" admissions of "well" infants highlighted by this study reflects poorly on the services available for well infant whose mothers are unable to care for them for whatever reason. We recommend regular audit of these infants in order to ensure efficient use of neonatal resources and to ensure optimum levels of neonatal intensive care.


Subject(s)
Birth Weight , Intensive Care Units, Neonatal/statistics & numerical data , Workload/statistics & numerical data , Female , Humans , Infant, Newborn , Infant, Newborn, Diseases/therapy , Male , Medical Audit , Perinatal Care , Retrospective Studies , Treatment Outcome
11.
Naunyn Schmiedebergs Arch Pharmacol ; 343(4): 344-52, 1991 Apr.
Article in English | MEDLINE | ID: mdl-1906582

ABSTRACT

Using the hemisected spinal cord of the neonate rat, the effects of altered external Ca, thyrotrophin-releasing hormone (TRH) and a number of antagonists were tested on depolarizations evoked by 5-hydroxytryptamine (5-HT). Responses of populations of motoneurones were recorded via a ventral root. 5-Hydroxytryptamine depolarizations were not Ca-dependent but were enhanced in amplitude in Ca-free solutions. Raised Mg reversed this enhancement. 5-Hydroxytryptamine depolarizations persisted in the presence of Mn (1.5-3 mmol/l). TRH depolarized motoneurones; there was no evidence of modulation of 5-HT responses on concurrent application of TRH. Ritanserin (0.1 mumol/l) had a modest blocking action on 5-hydroxytryptamine depolarizations reducing the maximum; 1 mumol/l ritanserin caused a greater antagonism which was unsurmountable (pIC50 5.2). Ritanserin (0.1 or 1 mumol/l) did not depress responses to noradrenaline (NA). Ketanserin (0.1 mumol/l) caused a blockade of slow onset, equilibrium with the receptors requiring 1 h. Blockade by 0.01, 0.1 and 1 mumol/l ketanserin was concentration-dependent (pIC50 6.2). Ketanserin 1 mumol/l, but not at lower concentrations, depressed noradrenaline responses. Mianserin (0.1 mumol/l) also caused a blockade of slow onset; 0.1 or 1 mumol/l produced a flattening of the 5-hydroxytryptamine concentration-response curve but did not depress noradrenaline responses (pIC50 4.7). The pIC50 for spiperone was 8.0. DOI (10-100 mumol/l) had no detectable agonist action but at concentrations of 0.01 and 0.1 mumol/l it acted as an antagonist. Equilibration with the receptors occurred over 2 h. DOI (0.01 mumol/l) depressed 5-hydroxytryptamine but not noradrenaline responses; higher concentrations of DOI also depressed noradrenaline responses.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Calcium/pharmacology , Epinephrine/pharmacology , Motor Neurons/drug effects , Serotonin Antagonists/pharmacology , Serotonin/physiology , Spinal Cord/drug effects , Animals , Animals, Newborn , Rats , Receptors, Serotonin/drug effects , Spinal Cord/metabolism , Thyrotropin-Releasing Hormone/physiology
12.
Neuropharmacology ; 28(12): 1399-404, 1989 Dec.
Article in English | MEDLINE | ID: mdl-2559351

ABSTRACT

Superfusion of the hemisected lumbar spinal cord in the neonate rat with solutions containing 10(-6) to 10(-3) M noradrenaline (NA), elicited graded depolarizations recorded from ventral roots, with a mean EC50 value of 15.1 +/- 1.5 microM (mean +/- SEM, n = 37). Repeated concentration-response curves to NA could be determined from the same preparation. Adrenaline had a similar depolarizing action (EC50 9.9 +/- 1.7 microM, n = 11). Blockade of neuronal uptake of NA by desipramine (2 x 10(-6) M) caused some potentiation of submaximal responses to NA and shifted the EC50 to 6.0 +/- 1.7 microM (mean +/- SEM, n = 14). The depolarizing response to NA was unaffected by DL-propranolol (10(-7) M) or yohimbine (10(-7) M). Prazosin (5 x 10(-9), 10(-8) and 10(-7) M) reduced the responses and caused a progressive rightward shift of the concentration-response curve. The onset of blockade by prazosin was slow, superfusion for at least 90-120 min being required before the blockade plateaued. Prazosin (5 x 10(-9) and 10(-8) M) caused a surmountable blockade, the apparent pA2 being 8.3 +/- 0.2 (mean +/- SEM, N = 9). Depolarizations induced by NA were also antagonised by phentolamine (10(-6) M). An initial hyperpolarizing response to NA was unmasked after exposure to prazosin in 90% of preparations and was associated with a reduction in the spontaneous activity of the motorneurones. Both the hyperpolarization and reduction in spontaneous activity were attenuated by yohimbine.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Animals, Newborn/physiology , Motor Neurons/drug effects , Norepinephrine/pharmacology , Receptors, Adrenergic, alpha/physiology , Animals , Catecholamines/metabolism , Electric Stimulation , In Vitro Techniques , Membrane Potentials/drug effects , Neurotransmitter Uptake Inhibitors/pharmacology , Norepinephrine/antagonists & inhibitors , Prazosin/pharmacology , Rats
13.
Neuropharmacology ; 28(6): 625-34, 1989 Jun.
Article in English | MEDLINE | ID: mdl-2755565

ABSTRACT

Superfusion of hemisected lumbar spinal cord of the neonatal rat with solutions containing 10(-6) to 10(-3) M 5-hydroxytryptamine (5-HT) elicited depolarizations of graded amplitude which were recorded from motorneurons through a ventral root. Maximum responses (amplitude 1.0 +/- 0.1 mV, mean +/- SEM, n = 30) were evoked by 10(-4) M 5-HT. Repeated concentration-response curves could be determined from the same preparation. There was no involvement of 5-HT2 receptors in the depolarizing response to 5-HT, since neither ritanserin nor ICI 169, 369 showed any antagonist action. Amongst agents with activity at 5-HT1A sites, the selective 5-HT1A receptor agonist, 8-hydyroxy-2-(di-N-propylamino) tetralin (8-OH-DPAT), neither mimicked the action of 5-HT nor antagonised it, while spiperone (10(-8)-10(-7 M) antagonised responses to 5-HT in a concentration-related manner. Responses to 10(-4) M noradrenaline, used as a control depolarizing agent, were unaffected by spiperone. The onset of blockade by spiperone was slow, 1 hr being required for equilibration of the tissue with antagonist. The blockade was surmountable by larger concentrations of 5-HT. Concentration-response curves to 5-HT were shifted to the right in an approximately parallel manner by spiperone. The dose ratios measured from these curves at the EC50 level, yielded an apparent pA2 of 8.24 +/- 0.14 (mean +/- SEM, n = 15), although the Schild plot of the data had a slope less than unity. The lack of activity of the selective 5-HT1B receptor agonist, RU 24969, and the 5-HT1B receptor antagonists, (+/-) cyanopindolol and quipazine, indicated that 5-HT1B receptors were not involved in the 5-HT response of motorneurones to 5-HT. Mesulergine, metergoline and cyproheptadine also antagonised responses of motorneurones to 5-HT, producing a surmountable blockade. Mesulergine (10(-8), 3 x 10(-8) and 10(-7) M caused a progressive rightward shift of the concentration-response curves, but 10(-7) M depressed the maximum response to 5-HT. Responses to noradrenaline were not affected by these concentrations of mesulergine. The apparent pA2 for blockade of 5-HT responses by mesulergine, calculated from experiments in which there was a parallel displacement of the concentration-response curves, was 8.75 +/- 0.11 (mean +/- SEM, n = 10).(ABSTRACT TRUNCATED AT 400 WORDS)


Subject(s)
Animals, Newborn/physiology , Motor Neurons/physiology , Neuromuscular Depolarizing Agents/pharmacology , Receptors, Serotonin/drug effects , Serotonin/pharmacology , Animals , Electric Stimulation , Motor Neurons/drug effects , Norepinephrine/pharmacology , Rats , Serotonin Antagonists/pharmacology , Spinal Cord/physiology , Spiperone/pharmacology , Time Factors
14.
Br J Pharmacol ; 94(4): 1101-14, 1988 Aug.
Article in English | MEDLINE | ID: mdl-3207976

ABSTRACT

1. Superfusion of isolated hemisected spinal cord from neonate rats with 5-hydroxytryptamine (5-HT) (10(-6) to 10(-3) M) evoked concentration-related depolarizations. The maximal depolarization elicited by a concentration of 10(-4) M was 1.0 +/- 0.1 mV (mean +/- s.e.mean, n = 30). Noradrenaline in a similar range of concentrations also elicited depolarizations. 2. The depolarizations probably originate in motoneurones as a result of direct interaction of the amines with these cells, since responses were unaltered by tetrodotoxin (10(-7) M) or Ca2+-free/Mg2+-rich medium. 3. 5-Carboxamidotryptamine (5-CT), S(+)-alpha-methyl-5-hydroxytryptamine (alpha-Me5-HT) and 5-methoxytryptamine (5-MeOT) evoked similar depolarizations to 5-HT. Tryptamine evoked depolarizations of smaller maximal amplitude. 5-Hydroxytryptophan, 2-methyl-5-hydroxytryptamine, 8-hydroxy-2-(di-N-propylamino) tetralin hydrobromide (8-OH-DPAT) and 5-methoxy-3-[1,2,3,6-tetrahydro-4-pyridinyl]-1-H-indole succinate (RU 24969) had no depolarizing action. 4. Concentration-response (CR) curves were determined for 5-HT, 5-CT, alpha-Me5-HT, 5-MeOT and tryptamine. The ED50 value for 5-HT was 20.5 +/- 1.2 microM. The equipotent molar ratios (EPMRs) for 5-CT and alpha-Me5-HT were close to unity, while 5-MeOT was approximately 3 times and tryptamine 13 to 14 times less potent than 5-HT. 5. The relative agonist potency of 5-HT with respect to other tryptamine analogues capable of depolarizing motoneurones was increased when 5-HT uptake was blocked by citalopram (10(-7) M). In the presence of citalopram, 5-HT was 2.7 times more potent than alpha-Me5-HT and 16.9 times more potent than 5-CT. The apparent order of potency was 5-HT greater than alpha-Me5-HT greater than 5-CT (greater than 5-MeOT much greater than tryptamine). 6. The monoamine oxidase inhibitor, pargyline (5 x 10(-4) M), had no effect on depolarizations to 5-HT, 5-CT or alpha-Me5-HT. 7. Methiothepin, 1 alpha H, 3 alpha, 5H-tropan-3-yl-3,5-dichlorobenzoate methanesulphonate (MDL 72222) and [3 alpha-tropanyl]-1H-indole-3-carboxylic acid ester hydrochloride (ICS 205-930) had no effect on 5-HT depolarizations elicited in motoneurones. Ketanserin (0.75 x 10(-7) M to 10(-6) M) showed modest antagonistic action and depressed maximal response amplitude; the pIC50 was 6.5. 8. Methysergide (10-8 to 10- 7M) was a potent antagonist of responses to 5-HT. CR curves were displaced to the right and flattened in the presence of the antagonist. The pIC5o assessed from the effect on depolarizations evoked by 5-HT 1O-4M was 7.5. 9. It is concluded that 5-HT acts directly to depolarize mammalian spinal motoneurones through receptors that are also activated by 5-CT, alpha-MeS-HT and 5-MeOT and are blocked by methysergide. The receptor profile, although not 5-HT3-like, does not clearly coincide with that for either 5-HT1-like or 5-HT2 receptors.


Subject(s)
Animals, Newborn/physiology , Serotonin/pharmacology , Spinal Cord/drug effects , Animals , Citalopram/pharmacology , Electrodes , In Vitro Techniques , Ketanserin/pharmacology , Male , Methysergide/pharmacology , Neuromuscular Depolarizing Agents/pharmacology , Norepinephrine/pharmacology , Pargyline/pharmacology , Rats , Serotonin Antagonists/pharmacology
15.
J Physiol ; 376: 47-61, 1986 Jul.
Article in English | MEDLINE | ID: mdl-3795080

ABSTRACT

Cross-correlation analysis of unitary neuronal discharges has been used to study the linkage between alpha- and gamma-motoneurones coactivated during the flexion reflex of the semitendinosus muscle in the decerebrated spinal cat. A flexion reflex was elicited by firm grip or squeeze of the ipsilateral heel, shank or foot. The stimulus excited the discharges of both alpha- and gamma-motoneurones and increased the frequency of discharge of those gamma-motoneurones that had shown a background discharge prior to intentional stimulation. Short-term synchrony was present between a high proportion of semitendinosus gamma-motoneurones both for background discharges (sixteen out of nineteen pairs) and during the flexion reflex (thirteen out of fifteen pairs). All nineteen pairs of alpha-motoneurones examined during the flexion reflex showed short-term synchrony of discharge. Few alpha-motoneurones displayed background discharges but synchrony was observed in the two instances studied. The degree of synchrony was measured as the ratio (kappa) of the total counts contributing to the peak of the correlogram over the number expected by chance alone. The ratio was higher when the average frequency of motoneurone discharge was low. Kappa was generally higher for alpha-motoneurone pairs than for gamma-motoneurone pairs. The higher degree of synchrony for alpha-motoneurones reflected their lower discharge rates. During the flexion reflex the degree of synchrony between gamma-motoneurones was greater than expected for that same discharge rate in the absence of intentional stimulation. Only twenty-seven out of forty pairings of an alpha- with a gamma-motoneurone showed a significant degree of synchrony of discharge. On average, the degree of synchrony for alpha/gamma pairs was lower than that for either alpha/alpha or gamma/gamma pairings at the equivalent discharge rate. The results support the conclusion that coactivation of alpha- and gamma-motoneurones during the flexion reflex occurs largely through independent sets of interneurones. The possibility is discussed that those alpha-motoneurones which showed short-term synchrony with gamma-motoneurones were skeleto-fusimotor (beta-motoneurones) in nature.


Subject(s)
Motor Neurons/physiology , Reflex, Stretch , Action Potentials , Animals , Cats , Decerebrate State/physiopathology , Foot/physiology , Motor Neurons, Gamma/physiology , Time Factors
16.
J Comp Neurol ; 230(1): 88-98, 1984 Nov 20.
Article in English | MEDLINE | ID: mdl-6096416

ABSTRACT

Transport of horseradish peroxidase (HRP) through somatic and visceral nerve fibres was used to study the patterns of termination of somatic and visceral primary afferent fibres within the lower thoracic segments of the cat's spinal cord. A concentrated solution of HRP was applied for at least 5 hours to the central end of the righ greater splanchnic nerve and of the left T9 intercostal nerve of adult cats. Some animals remained under chloralose anaesthesia for the duration of the HRP transport times (up to 53 hours) whereas longer HRP application and transport times (4-5 days) were allowed in animals that recovered from barbiturate anaesthesia. Somatic afferent fibres and varicosities (presumed terminals) were found in laminae I, II, III, IV, and V of the ipsilateral dorsal horn and in the ipsilateral Clarke's column. The density of the somatic projection was particularly high in the superficial dorsal horn. In parasagittal sections of the cord, bundles of somatic fibres were seen joining the dorsal horn from the dorsal roots via the dorsal columns and Lissauer's tract. A medio-lateral somatotopic arrangement of somatic afferent terminations was observed, with afferent fibres from the ventral parts of the dermatome ending in the medial dorsal horn and afferent fibres from the dorsal parts of the dermatome ending in the lateral dorsal horn. The total rostro-caudal extent of the somatic projection through a single spinal nerve was found to be of 2 and 2/3 segments, including the segment of entry, the entire segment rostral to it and two-thirds of the segment caudal to it. A lateral to medial shift in the position of the somatic projection was observed in the rostro-caudal axis of the cord. Visceral afferent fibres and varicosities (presumed terminals) were seen in laminae I and V of the ipsilateral dorsal horn. The density of the visceral projection to the dorsal horn was substantially lower than that of the somatic projection. Visceral afferent fibres reached the dorsal horn via Lissauer's tract and joined a lateral bundle of fine fibres that run along the lateral edge of the dorsal horn. The substantia gelatinosa (lamina II) appeared free of visceral afferent fibres. These results are discussed in relation to the mechanisms of viscero-somatic convergence onto sensory pathways in the thoracic spinal cord.


Subject(s)
Nerve Fibers/physiology , Spinal Cord/physiology , Afferent Pathways/physiology , Animals , Cats , Horseradish Peroxidase , Motor Neurons/physiology , Sympathetic Nervous System/cytology , Sympathetic Nervous System/physiology , Synaptic Transmission , Thorax
17.
J Comp Neurol ; 228(3): 422-31, 1984 Sep 20.
Article in English | MEDLINE | ID: mdl-6480920

ABSTRACT

Anterograde transport of horseradish peroxidase (HRP) through somatic and visceral nerves was used to estimate the proportions of somatic and visceral dorsal root ganglion (DRG) cells of the lower thoracic ganglia of the cat. A concentrated solution of HRP was applied for at least 5 hours to the central end of the right greater splanchnic nerve and of the left T9-intercostal nerve of adult cats. Some animals remained under chloralose anaesthesia for the duration of the HRP transport time (up to 53 hours) whereas longer HRP application and transport times (4-5 days) were allowed in animals that recovered from barbiturate anaesthesia. Visceral DRG cells were found in approximately equal numbers in all ganglia examined (T7-T11). Population estimates were obtained for the T8 and T9 ganglia where visceral DRG cells were found to be 6.2% (T8) and 5.2% (T9) of the total cell population. In contrast, somatic DRG cells were found in large numbers in the ganglia examined (T8 and T9) where they amounted to over 90% of the cell population. Measurement of cross-sectional areas and estimates of cell diameters of the DRG cells showed greater proportions of large somatic cells (diameter greater than 40 micron) than of large visceral cells. Similar distributions of cell size were found for both somatic and visceral DRG cells with diameters less than 40 micron. These results show that the proportion of visceral afferent fibres in the dorsal roots that mediate the spinal cord projection of the splanchnic nerve is very small. Since viscerosomatic convergence in the thoracic spinal cord is very extensive, the present results suggest considerable divergence of the visceral afferent input to the central nervous system.


Subject(s)
Ganglia, Spinal/cytology , Intercostal Nerves/cytology , Splanchnic Nerves/cytology , Thoracic Nerves/cytology , Animals , Cats , Female , Male , Neurons, Afferent/cytology
18.
Brain Res ; 294(2): 370-4, 1984 Mar 05.
Article in English | MEDLINE | ID: mdl-6200187

ABSTRACT

Transganglionic transport of horseradish peroxidase (HRP) has been used to study the anatomy of the central projection of somatic and visceral afferent fibers to the thoracic spinal cord of the cat. A dense concentration of somatic afferent fibers and terminals was found in laminae I and II of the dorsal horn and more scattered terminals were present in laminae III, IV and V and in Clarke's column. In contrast, visceral afferent fibers and terminals were found only in lamina I or reaching lamina V via a small bundle of fibers located in the lateral border of the dorsal horn. These results indicate that fine afferent fibers from viscera, unlike those of cutaneous origin, do not project to the substantia gelatinosa (lamina II) of the dorsal horn.


Subject(s)
Afferent Pathways/physiology , Intercostal Nerves/physiology , Spinal Cord/physiology , Splanchnic Nerves/physiology , Substantia Gelatinosa/physiology , Thoracic Nerves/physiology , Animals , Axonal Transport , Cats , Female , Horseradish Peroxidase , Male , Nerve Fibers/physiology , Thorax
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