Invited Review: Epigenetics in neurodevelopment.

Neural development requires the orchestration of dynamic changes in gene expression to regulate cell fate decisions. This regulation is heavily influenced by epigenetics, heritable changes in gene expression not directly explained by genomic information alone. An understanding of the complexity of epigenetic regulation is rapidly emerging through the development of novel technologies that can assay various features of epigenetics and gene regulation. Here, we provide a broad overview of several commonly investigated modes of epigenetic regulation, including DNA methylation, histone modifications, noncoding RNAs, as well as epitranscriptomics that describe modifications of RNA, in neurodevelopment and diseases. Rather than functioning in isolation, it is being increasingly appreciated that these various modes of gene regulation are dynamically interactive and coordinate the complex nature of neurodevelopment along multiple axes. Future work investigating these interactions will likely utilize 'multi-omic' strategies that assay cell fate dynamics in a high-dimensional and high-throughput fashion. Novel human neurodevelopmental models including iPSC and cerebral organoid systems may provide further insight into human-specific features of neurodevelopment and diseases.

Reliability of the assessment of peripheral muscle fatigue induced by high-intensity intermittent exercise.

AIM: Reliability of the assessment of peripheral fatigue induced in knee extensors by high-intensity intermittent running exercise (HIE) was established in ten amateur soccer players (age: 18±1, height: 178±5 cm, weight: 68±8 kg). METHODS: Transcutaneous electrical stimulations before and after HIE determined muscle contractile properties on three separate occasions (T1, T2 and T3), each separated by 7 days. RESULTS: No significant differences were noted for any of the parameters measured (P=0.101). The ICC values for peak torque (PT) varied from moderate to high, with the exception of PT at 10-Hz calculated comparing T2 vs. T1 (range PRE=0.78-0.92; POST=0.76-0.97). The ICC derived from PT percentage decrements data were all low, with the exception of PT decrements at 1 Hz calculated comparing T3 vs. T2 (ICC=0.85, moderate). The TE for all contractile parameters obtained using 1-Hz and 100-Hz electrical stimulations were below 10%, including some that demonstrated a TE lower than 5%. Muscle contractile properties determined using 10-Hz stimulations showed a higher level of TE (range: 3.2-15.1%). Similar results were obtained for maximal rate of torque development and torque relaxation. CONCLUSION: From the results of this study it can be concluded that muscle contractile properties express a good level of reliability in baseline and postexercise measures following familiarization.