Draft Genome Sequences of Pseudomonas Isolates Derived from Glyphosate-Treated Sediments.

Reported here are the genome sequences of Pseudomonas isolates that were derived from glyphosate-treated sediment microcosms. Genomes were assembled using workflows available through the Bacterial and Viral Bioinformatics Resource Center (BV-BRC). The genomes of eight Pseudomonas isolates were sequenced, with genomes ranging from 5.9 to 6.3 Mb.

Amplicons, Metagenomes, and Metatranscriptomes from Sediment and Water.

High fecal indicator bacterium (FIB) counts in water have been found to correlate with high sediment FIB counts. To determine the other bacterial populations in common between the two substrates, sediment and water samples from suburban waters known to be impacted by stormwater runoff were examined using next-generation sequencing.

Taxonomic Profiling of Microbes in Glyphosate-Treated Sediment Microcosms.

Here, we report the impact of glyphosate on bacterial populations in sediment microcosms, determined using 16S amplicon sequencing and shotgun metagenomics with source material from a suburban creek. The 16S amplicon and metagenomic data reveal that members of the genus Pseudomonas are increased by the treatment.

Complete Genome Sequences of Two Mycobacterium smegmatis Bacteriophages.

Mycobacterium smegmatis, an acid-fast bacterial species in the phylum Actinobacteria, has often been used as a substitute for pathogenic mycobacteria in research. Here, we describe the isolation and characterization of two M. smegmatis bacteriophages, Penelope2018 and Miniwave.

Complete Genome Sequences of Mycobacteriophages Apex and Gophee.

Apex and Gophee are mycobacteriophages directly isolated from soil using the host Mycobacterium smegmatis mc2155. Apex has a 71,244-bp double-stranded DNA (dsDNA) genome encoding 98 putative proteins, and Gophee has a 68,556-bp dsDNA genome encoding 101 putative proteins.

Genome Sequences of Mycobacteriophages Joselito, Patt, and Tydolla.

Mycobacteriophages Joselito, Patt, and Tydolla were isolated from different soil or water samples using Mycobacterium smegmatis mc2155 as the host. Each was obtained using direct isolation techniques, purified, and then sequenced using the deconvolution of genomes after en masse sequencing (DOGEMS) method.

Complete Genome Sequences of Cluster F Mycobacteriophages EleanorGeorge, Mattes, and Spikelee.

EleanorGeorge, Mattes, and Spikelee are mycobacteriophages isolated from different soil samples using Mycobacterium smegmatis mc2155 as the host. Each was obtained using direct isolation techniques, purified, and then sequenced. Based on sequence similarity, all three belong to the F1 subcluster and are temperate phages.

Molecular characterization of a gene encoding a cystatin expressed in the stems of American chestnut (Castanea dentata).

A cDNA clone with similarity to genes encoding cystatin was recently isolated from a cDNA library created using mRNA extracted from stem tissues of Castanea dentata (Marsh.) Borkh. (CASde:Pic1). All of the requisite motifs for inhibitory activity were found upon examination of the deduced amino acid sequence. Reverse transcription-polymerase chain reaction was used to detect the cystatin transcript in healthy stem, leaf and seed tissues, as well as in diseased tissues. Gene fragments encoding this putative cystatin were cloned from American and Chinese (Castanea mollissima Blume) chestnuts and a comparison of these sequences revealed significant differences within the intron, including deletions and alterations in restriction-enzyme sites. The long-term goal of this study is to determine whether the cystatin allele in Chinese chestnut correlates to a resistance gene and, if so, if this allele could be used to enhance resistance in American chestnut.