The effects of the high affinity antiestrogen, H1285, on uterine growth and morphology.

Experiments were performed to compare the agonistic and antagonistic properties of the high affinity antiestrogen, H1285, and the low affinity antiestrogens CI-628 and tamoxifen on rat uterine growth and morphology. Myometrial and stromal areas and endometrial epithelial cell heights were calculated. Whereas all antiestrogens displayed some degree of estrogenic response, only H1285 at 5 or 0.5 micrograms was an effective inhibitor of E2 induced proliferative responses in the myometrium, stroma, and endometrial epithelium. All antiestrogens caused tremendous endometrial hypertrophy.

An autoradiographic study of the distribution of fibers from the dorsal motor nucleus of the vagus to the digestive tube of the rat.

At the present time, complete agreement on the origin and course of parasympathetic preganglionic fibers to the alimentary canal has not been reached. The purpose of this study was to trace vagal fibers to the abdominal cavity and to follow the distribution of these fibers to the digestive tube. The technique used was to label neurons in the dorsal motor nucleus of the vagus (DMX) with 3H-leucine and then to follow the orthograde transport. 16 albino rats were used in this experiment. The right DMX in one group of rats and the left DMX in the other group was injected with 25 microCi of 3H-leucine in three injections. The injection sites and tissue sections from various areas of the digestive tube were processed for autoradiography. A heavy label was observed in the injection site and it could be traced down the vagus nerve through the thorax into the abdomen. Labelled vagal fibers were found in the parasympathetic ganglia of the stomach, small intestine and colon.

An orthograde labelling study of axons of the dorsal motor nucleus of the vagus to rat cardiac ganglia as demonstrated by autoradiography.

Central organization of the cardiac vagus has not been clarified. Retrograde changes produced in medulla oblongata neurons after section of vagal branches has favored the dorsal motor nucleus of the vagus (DMNX). Current information concerning the origin, course, and termination of vagal preganglionic fibers within cardiac ganglia is conflicting. The explicit purpose of this study was to determine if vagal fibers originated specifically within the DMNX proper. Fibers within the cardiac ganglia were labelled with 3H-leucine following injection into the DMNX. 12 adult albino rats were studied. DMNX were injected with 25 microCi 3H-leucine reconstituted to microliter. Animals were sacrificed by transcardial perfusion following a 4-day survival period. Serial cross-sections of the caudal pons, medulla oblongata, and thoracic viscera were processed for autoradiography. DMNX possessed a heavy incorporation of the radiochemical. Label was observed within the axons of the vagi. Cardiac ganglia contained labelled vagal fibers in close proximity to the postganglionic somata. Cardiac ganglia containing labelled preganglionic vagal axons were located in the cardiac plexuses and in the epicardium. Results show a labelled vagal preganglionic input to cardiac ganglia from the DMNX.

Irradiated salivary glands in the rhesus monkey. A light microscopic study.

Female rhesus monkeys (Macaca mulatta) received fractionated doses of orthovoltage irradiation to the submandibular and sublingual glands. Changes in the glands varied from serous cell degranulation and degeneration in the submandibular glands to acinar cell necrosis and fibrosis in the sublingual glands. Acute inflammation was absent in all irradiated glands. In all glands, the microvasculature appeared normal.

The orthograde labeling of postganglionic sympathetic cardiac nerves in the dog and cat as demonstrated by autoradiography.

Previous functional and anatomical techniques have characterized the cardiac nerves and plexuses. They cannot, however, determine the course of fibers arising from a specific ganglion. This study has found that the intraaxonal orthograde labeling of axons can be used to determine the course of postganglionic sympathetic cardiac fibers in the dog and cat. The canine left caudal cervical ganglion and the feline right stellate ganglion were exposed through appropriate thoracotomies. Each ganglion received multiple injections of tritiated leucine (500 muCi/animal). Following a 3--14-day survival period the anesthetized animals were sacrificed by vascular perfusion. The injected ganglia, extracardiac nerves and selected portions of the heart were processed for autoradiography. Autoradiographs from the dog demonstrated labeled postganglionic sympathetic nerves in the extracardiac plexus, left atrial epicardium and the epicardium beneath the coronary sulcus. Labeled nerves in the cat heart were found within the epicardial layers and associated with blood vessels in the left ventricular myocardium. Neither myelinated fibers traveling through the injection site nor intrinsic cardiac ganglion cells were labeled, although the latter were often closely approximated to heavily labeled fibers.