Microcosm experiments and kinetic modeling of glyphosate biodegradation in soils and sediments.

Glyphosate (GLP) is one of the most widely-used herbicides globally and its toxicity to humans and the environment is controversial. GLP is biodegradable, but little is known about the importance of site exposure history and other environmental variables on the rate and pathway of biodegradation. Here, GLP was added to microcosms of soils and sediments with different exposure histories and these were incubated with amendments of glucose, ammonium, and phosphate. GLP concentrations were measured with a newly-developed HPLC method capable of tolerating high concentrations of ammonium and amino acids. GLP biodegradation occurred after a lag-time proportional to the level of GLP pre-exposure in anthropogenically-impacted samples (soils and sediments), while no degradation occurred in samples from a pristine sediment after 180 days of incubation. Exposure history did not influence the rate of GLP degradation, after the lag-time was elapsed. Addition of C, N, and P triggered GLP degradation in pristine sediment and shortened the lag-time before degradation in other samples. In all microcosms, GLP was metabolised into aminomethylphosphonic acid (AMPA), which was highly persistent, and thus appears to be a more problematic pollutant than GLP. Bacterial communities changed along the gradients of anthropogenic impacts, but in some cases, taxonomically very-similar communities showed dramatically different activities with GLP. Our findings reveal important interactions between agriculturally-relevant nutrients and herbicides.

Glyphosate dispersion, degradation, and aquifer contamination in vineyards and wheat fields in the Po Valley, Italy.

Biodegradation of glyphosate (GLP) and its metabolite aminomethylphosphonic acid (AMPA) was numerically assessed for a vineyard and a wheat field in the Po Valley, Italy. Calculation of the Hazard Quotient suggested that GLP and AMPA can pose a risk of aquifer contamination in the top 1.5 m depth within 50 years of GLP use. Numerical results relative to soil GLP and AMPA concentrations, and GLP age, half life, and turnover time show that GLP was equivalently removed through hydrolysis and oxidation, but the latter produced AMPA. Biodegradation processes in the root zone removed more than 90% of applied GLP and more than 23% of the produced AMPA between two consecutive applications. Doubling organic carbon availability enhanced GLP and AMPA biodegradation, especially GLP hydrolysis to sarcosine. This work highlights that GLP and AMPA removal is controlled by soil water dynamics that depend on ecohydrological boundary conditions, and by carbon sources availability to biodegraders.