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New Microbiol ; 40(2): 151-154, 2017 Apr.
Article in English | MEDLINE | ID: mdl-28368078

ABSTRACT

This report describes a case of congenital toxoplasmosis in a newborn in Southern Italy. A pregnant mother had been admitted at the 20th week of her pregnancy on account of pharyngodynia and laterocervical lymphadenopathy. Although serological testing of the mother's serum documented a seroconversion with positive IgG and IgM anti-Toxoplasma antibodies during II trimester, the woman refused to perform prenatal diagnosis for congenital toxoplasmosis. Fetal ultrasound scan already showed mild asymmetrical triventricular hydrocephaly and cerebral calcifications. After birth, real-time PCR on cerebrospinal fluid and blood samples of the newborn showed a positive result for 529bp-repeat element DNA of T. gondii, In addition brain magnetic resonance imaging and computed tomography showed a characteristic diffuse brain tissue loss associated with hydrocephalus. For the first time molecular characterization of T. gondii isolate was performed directly from the newborn's CSF samples by using nested-PCR-RFLP of sag-2 and pk1 genes. The PCR-RLFP analysis revealed that the isolate belongs to the clonal type II, the predominant lineage causing human toxoplasmosis, as confirmed by DNA sequencing.


Subject(s)
Toxoplasma/genetics , Toxoplasmosis, Congenital/parasitology , Adult , Antibodies, Protozoan/blood , Base Sequence , Cerebrospinal Fluid/parasitology , DNA, Protozoan/cerebrospinal fluid , DNA, Protozoan/chemistry , Female , Genotype , Genotyping Techniques , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Infant, Newborn , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Pregnancy , Toxoplasma/classification , Toxoplasma/immunology , Toxoplasmosis, Congenital/cerebrospinal fluid , Toxoplasmosis, Congenital/diagnostic imaging
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