ABSTRACT
Purine nucleotides were studied in human peripheral blood lymphocytes from normal subjects and patients with chronic B-cell lymphocytic leukemia (B-CLL). Nucleotide content was determined by high performance liquid chromatography (HPLC). The overall rate of purine nucleotide synthesis was measured following the incorporation of 14C-formate into the nucleotides of a lymphocytic suspension. Results indicate a substantially reduced rate of purine nucleotide metabolism.
Subject(s)
Leukemia, Lymphocytic, Chronic, B-Cell/metabolism , Lymphocytes/metabolism , Purine Nucleotides/metabolism , Aged , Aged, 80 and over , Chromatography, High Pressure Liquid , Humans , Middle AgedSubject(s)
HIV Infections/metabolism , HIV-1 , Lymphocyte Subsets/metabolism , Purine Nucleotides/metabolism , Adenine/blood , Adenosine Diphosphate/blood , Adenosine Triphosphate/blood , Adult , CD4-Positive T-Lymphocytes/metabolism , Female , Guanine/blood , Guanosine Diphosphate/blood , Guanosine Triphosphate/blood , HIV Seropositivity/blood , Humans , Male , Middle Aged , T-Lymphocyte Subsets/metabolismSubject(s)
Leukemia, Lymphocytic, Chronic, B-Cell/blood , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood , Leukemia, Myeloid, Acute/blood , Leukocytes/metabolism , Purine Nucleotides/blood , Adenine Nucleotides/blood , Aged , Aged, 80 and over , Cell Survival , Chromatography, High Pressure Liquid , Female , Guanine Nucleotides/blood , Humans , Inosine Monophosphate/blood , Leukocytes/cytology , Leukocytes/pathology , Male , Middle Aged , NAD/blood , Neutrophils/metabolism , Reference ValuesSubject(s)
Leukemia, Lymphocytic, Chronic, B-Cell/blood , Leukocytes/metabolism , Purine Nucleotides/blood , Adenine Nucleotides/blood , Guanine Nucleotides/blood , Humans , Inosine Monophosphate/blood , Leukocytes, Mononuclear/metabolism , NAD/blood , Neutrophils/metabolism , Purine Nucleotides/isolation & purification , Reference ValuesABSTRACT
The purine nucleotide content was examined in various cells before and after HIV-1 virus infection: healthy peripheral blood lymphocytes (PBL) and cultured PBL after infection; the PBL of asymptomatic, ARC and AIDS patients. In all cases, changes in purine nucleotide concentrations were observed. The pattern of purine nucleosides and nucleobases was also evaluated by HPLC in PBL of controls and patients. The analysis was integrated by following the incorporation of a labelled precursor ([14C]formate) into purine nucleotides, which was investigated as an indication of the rate of purine metabolism in these cells. Many interesting variations in the catabolic and of anabolic pathways were observed, demonstrating that the viral penetration affects purine nucleotide metabolism. These results suggest interesting perspectives in AIDS research.
Subject(s)
AIDS-Related Complex/metabolism , Acquired Immunodeficiency Syndrome/metabolism , HIV Seropositivity/metabolism , HIV-1 , Lymphocytes/metabolism , Purine Nucleotides/metabolism , Adult , Cell Survival , Female , Humans , Kinetics , Male , Middle Aged , Pyrimidine Nucleotides/metabolismABSTRACT
Anion-exchange, high performance liquid chromatography was used to determine purine nucleotides in lymphocytes of healthy males and females of various ages. We observed a considerable dispersion of values which were unrelated to age or sex, possibly linked to various states of activation of circulating lymphocytes and to other unknown factors.
