Safety assessment of gasification biochars using Folsomia candida (Collembola) ecotoxicological bioassays.

Biochar is a product of the thermal decomposition of biomass under a limited supply of oxygen and can be deriving from pyrolysis or gasification. As the product is rich in highly recalcitrant carbon, it has been proposed as a soil amendment to improve soil fertility and to stock carbon in soils. However, the contaminant compounds present in biochar could represent potential environmental threats. The gasification biochar is a promising by-product, but its effects on soil microarthropods are still nearly unknown. The aim of this study was to assess, using a prognosis approach, any ecotoxicological consequences of four biochars (conifer, poplar, grape marc, and wheat straw) on the springtail Folsomia candida. This was assessed through a series of tests: an avoidance behavior test, a survival and reproduction test, and a test based on the hatching of eggs. Biochars were tested at different concentrations (pulverized and diluted w/w with an artificial standard soil). The results showed that the springtails did not tend to avoid the biochars' substrates up to the rate of 2-5%, but any higher levels of concentration caused the animals to keep away from it. While mortality was negatively affected only in the grape marc biochar, reproduction was significantly reduced in all biochars considered. The hatching of the eggs was anticipated at even the lowest concentrations of herbaceous biochars, while a severe delay was observed in both concentrations tested of the conifer biochar. The endpoints considered were negatively affected by pH, polycyclic aromatic hydrocarbons, and heavy metals (in order of importance). The findings confirmed the potential adverse effects that gasification biochars could have on soil microarthropods and demonstrated the necessity of introducing these tests into biochar characterization protocols.

Assessing biochar ecotoxicology for soil amendment by root phytotoxicity bioassays.

Soil amendment with biochar has been proposed as effective in improving agricultural land fertility and carbon sequestration, although the characterisation and certification of biochar quality are still crucial for widespread acceptance for agronomic purposes. We describe here the effects of four biochars (conifer and poplar wood, grape marc, wheat straw) at increasing application rates (0.5, 1, 2, 5, 10, 20, 50% w/w) on both germination and root elongation of Cucumis sativus L., Lepidium sativum L. and Sorghum saccharatum Moench. The tested biochars varied in chemical properties, depending on the type and quality of the initial feedstock batch, polycyclic aromatic hydrocarbons (PAHs) being high in conifer and wheat straw, Cd in poplar and Cu in grape marc. We demonstrate that electrical conductivity and Cu negatively affected both germination and root elongation at ≥5% rate biochar, together with Zn at ≥10% and elevated pH at ≥20%. In all species, germination was less sensitive than root elongation, strongly decreasing at very high rates of chars from grape marc (>10%) and wheat straw (>50%), whereas root length was already affected at 0.5% of conifer and poplar in cucumber and sorghum, with marked impairment in all chars at >5%. As a general interpretation, we propose here logarithmic model for robust root phytotoxicity in sorghum, based on biochar Zn content, which explains 66% of variability over the whole dosage range tested. We conclude that metal contamination is a crucial quality parameter for biochar safety, and that root elongation represents a stable test for assessing phytotoxicity at recommended in-field amendment rates (<1-2%).

Germination and root elongation bioassays in six different plant species for testing Ni contamination in soil.

In vitro short-term chronic phytotoxicity germination and root elongation test were applied to test the effects of nickel (Ni) in seed germination and root elongation in six plants species: Cucumis sativus (Cucurbitaceae), Lepidium sativum and Brassica nigra (Brassicaceae), Trifolium alexandrinum and Medicago sativa (Fabaceae), Phacelia tanacetifolia (Boraginaceae). A naturally Ni rich soil was used to compare the results obtained. Unlike root elongation, germination was not affected by Ni in any of the six species tested. EC50 values, calculated on the root elongation, showed that Ni toxicity decreases in the following order: P. tanacetifolia > B. nigra > C. sativus > L. sativum > M. sativa > T. alexandrinum. The test conducted using soil elutriate revealed a significantly lower effect in both seed germination and root elongation when compared to the results obtained using untreated soil. Conversely, the test performed on soil confirmed the high sensitivity of C. sativus, P. tanacetifolia and L. sativum to Ni.