ABSTRACT
La obesidad y la prevalencia de la diabetes han aumentado progresivamente en los últimos decenios, lo cual ha conllevado a diversas recomendaciones de la Organización Mundial de la Salud (OMS) para mejorar la dieta e incorporar hábitos saludables. Con el objetivo de limitar los azúcares agregados, reducir las calorías y modular la respuesta glucémica, la industria ha implementado distintas alternativas, entre las cuales ciertos azúcares raros presentan un gran potencial. La alulosa, como sustituto de azúcares tradicionales y edulcorantes, confiere características sensoriales similares a las de los productos con sacarosa y proporciona los beneficios nutricionales esperados. El objetivo de este trabajo fue revisar los conocimientos, hasta la fecha, sobre aspectos biológicos, legales y tecnológicos de la alulosa
The obesity and prevalence of type 2 diabetes have progressively increased in the recent decades, which has led to various recommendations from the World Health Organization (WHO) to improve diet and healthy habits. With the objective of limiting added sugars, reducing calories, and modulating the glycemic response, the industry has implemented different alternatives, among which certain rare sugars have great potential. Allulose as a substitute for traditional sugars and sweeteners confers sensory characteristics like those of products with sucrose and provides the expected nutritional benefits. The aim of this work was to look over the knowledge, to date, on biological, legal, and technological aspects of allulose
Subject(s)
Sweetening Agents , Glycemic Index , SucroseABSTRACT
The effect of different doses of UV-C light (5.3, 8.3 and 11.4 kJ/m2) on native mycobiota and Botrytis cinerea incidence, micro and ultrastructure, biomechanical properties and weight loss of blueberry fruit cv. O'Neal during 20 days of storage at 8 ± 1 °C was evaluated. Decay incidence was significantly reduced by all UV-C light doses for both, native mycobiota and inoculated B. cinerea. The highest UV-C dose studied (11.4 kJ/m2) was the most effective indelaying the onset of fungal and B. cinerea infection (6 and 4 days, respectively). UV-C irradiation caused some distinctive changes in fruit structure characterized by redistribution, alteration and partial removal of epicuticular waxes, reinforcement of epicarp cell walls, and modifications in the cuticle. Biomechanical parameters were not affected by UV-C treatments excepting at day 15 where irradiated samples showed higher values of rupture force (FR) and deformation (D). Structure changes partially explained the significant increase in weight loss, FR and D values in irradiated fruit after 15 days of storage. UV-C irradiation could be an alternative for delaying and reducing fungal infection. However, postharvest shelf-life of irradiated blueberries could be limited by the negative effect on weight loss.
ABSTRACT
This work aimed to evaluate the effect of ozone washing (maximum concentration 3.5 mgL-1- 5 and 15 min) on Botrytis cinerea decay, physicochemical parameters, bioactive compounds, in vitro and in vivo antioxidant activity, and sensory properties of strawberries cv. Albion throughout refrigerated storage at 5 ± 1 °C. A 5 min long ozonation delayed the onset of B. cinerea infection by 4 days, and significantly reduced its incidence as storage progressed (~17 % lesser than in control at day 8), without impairing physicochemical parameters or sensory quality. This treatment did not affect the antioxidant activity of strawberry extracts neither in in vitro (ORAC and ABTS assays) or in vivo assays using Caenorhabditis elegans as a model organism. Higher ozone doses did not achieve greater reduction of B. cinerea decay throughout the storage period. This study demonstrated that exposing strawberries to a 5 min long aqueous ozone treatment could extend their storability at 5 °C.
ABSTRACT
Botrytis cinerea causes postharvest spoilage in important crops such as strawberry and other berries. Pulsed light (PL) treatment could be an environmentally friendly postharvest alternative to synthetic fungicides in berries. Cultivability, physiological state, ultrastructure of Botrytis cinerea suspended in peptone water and irradiated with PL (fluenceâ¯=â¯1.2 to 47.8â¯J/cm2) were investigated by using conventional plate count technique, flow cytometry analysis (FCM) and transmission electron microscopy. In addition, PL effect on B. cinerea development in artificially contaminated strawberries throughout storage at (5⯱â¯1) °C was evaluated. PL reduced fungus' ability to form colonies on agarized culture media. Survival curve fitted with the Weibullian model evidenced a wide distribution of conidia sensitivity to PL. FCM showed that most of irradiated conidia entered in a viable non-culturable state, although a subpopulation without esterase activity and compromised membranes and a subpopulation with active esterase and intact membranes were also detected. PL attacked multiple targets in B. cinerea. Ultrastructural changes varied with the dose and within the conidia population, supporting FCM results. Damage included plasmalemma detachment from cell wall, cytoplasm collapse, and vacuolization of cytoplasm, disruption of cell wall and plasmalemma with massive loss of cytoplasm and/or disruption of organelles. In strawberries artificially contaminated with B. cinerea, a 2-day delay on the onset of the infection and a lower incidence in PL-treated strawberries (11.9 and 23.9â¯J/cm2) compared to control (16-20%) up to 10â¯days of cold storage was observed. Results indicated that PL significantly reduces B. cinerea growth in peptone water and in inoculated strawberries. However, other preservation factor(s) in combination would be needed to increase PL action for a better control of this fungus.
