Prevalence of the cagA Virulence Factor Varies by Race among H. pylori-infected Patients Undergoing Upper Endoscopy.

INTRODUCTION: We designed a race-conscious study to assess the presence of Helicobacter pylori (Hp) virulence factor cagA in a retrospective cohort of patients with active Hp infection. METHODS: We compared cagA status by race in gastric tissue samples from 473 patients diagnosed with active Hp infection from 2015-2019. RESULTS: Hp+ Black patients were two times more likely to be cagA+ than Hp+ White patients (82% vs. 36%, P < .0001). DISCUSSION: Presence of cagA is common among endoscopy patients with active Hp infection; appropriate testing and treatment of Hp can both reduce gastric cancer risk and address health disparities.

Disaggregating the influence of maturity status on training, anthropometric, performance, skeletal periphery, and hormonal factors in athletic boys.

Maturity offset (i.e., age from peak height velocity [PHV]) is widely used to assess maturational status among youth athletes, but details on the skeletal periphery, hormones and training factors are lacking. More precision is also needed to explicate the timing, tempo, and sequence of growth-related events. These gaps were addressed in a cross-sectional study. One hundred and two athletic boys (aged 14.1 ± 0.5 years) were evaluated for training details, salivary testosterone and cortisol, height, body mass, body mass index, body fat, fat-free mass (FFM), hand and carpal bone area, bone mineral content (BMC) and density (BMD), and countermovement jump performance. Participants were assigned to circa-PHV (N = 56) and post-PHV (N = 46) groups for comparisons, before data pooling and disaggregation using generalized additive and linear regression models. The older post-PHV group had a larger body size, more testosterone, and better performance and skeletal outcomes, whilst weekly training and school-based exercise favored the younger circa-PHV group (all p < 0.001). Smoother plots verified these differences via linear, or weakly non-linear, associations. Maturity offset was predicted (R2 = 0.848) by the linear combination of FFM (42.0 % relative contribution), hand BMD (31.5 %), chronological age (16.0 %), testosterone (6.7 %), and training hours (3.8 %). In conclusion, athletic boys who presented at different stages of maturity (-0.98 to 2.84 years from PHV) also differed on many developmental features. Most variables increased at constant, or near-constant, tempo with a higher maturity offset, with FFM and hand BMD emerging as the strongest linear predictors of maturational status.

Disentangling the dynamic interplay between muscle damage and energetics in male boxers during a short training block.

Boxing is a combat sport linked to muscle damage (e.g., soreness, rising creatine kinase [CK]) and energetic biomarkers (e.g., urea, glucose). These factors have not, however, been examined dynamically in terms of day-to-day, lagged and reciprocal effects during normal training. This study investigated the dynamic interplay between muscle damage and energetics in male boxers during a short training block. Thirteen amateur boxers were monitored over 16 consecutive days during early-season training. The participants were assessed each morning for plasma CK, urea, glucose, and creatinine (days 1 and 16 only) concentrations, before self-reporting muscle soreness (1-10 scale). Within-person contemporaneous (lag-0) and temporal (lag-1) networks were estimated using multilevel vector autoregression. Muscle soreness, CK, urea, and glucose presented different trajectories with training, but with some heterogeneity reflecting within-person variances (47% to 78%). The contemporaneous network yielded a significant positive edge (or correlation) between CK and soreness (r = 0.44), along with negative CK-glucose and glucose-urea edges. More significant edges emerged in the temporal network, with soreness linked to CK (r = 0.19), glucose (r = -0.28) and urea (r = 0.22), whilst the CK-glucose edge sign switched. In summary, daily fluctuations in muscle damage and energetic activity, which presented in a normal physiological range, were highly variable among boxers during early-season training. Within-person networks indicated some interrelatedness between CK, soreness, urea, and glucose, although the nature and presence of these relationships were contingent on temporal ordering. These inconsistences reflect the pleiotropy of energetic biomarkers in training and recovery.

Mental Rehearsal Improves Passing Skill and Stress Resilience in Rugby Players.

PURPOSE: Mental rehearsal is commonly employed, with positive visualization proposed to enhance complex skill performance. Additionally, video stimulus has been associated with enhanced kinesthetic sensations and rapid hormone fluctuations that may contribute to enhancing mental rehearsal and the conscious and unconscious emotional state for skill execution. Here, we assessed the impact of a 15-minute mental rehearsal intervention on rugby-specific tasks and the associated hormone profile. METHODS: Professional rugby players (N = 10) volunteered for a randomized crossover study. They completed three 15-minute preparatory phases (positive or negative video-guided mental rehearsal or self-directed mental rehearsal alone) prior to an exercise stressor and rugby-specific passing task. Salivary testosterone and cortisol were monitored to assess stress responses. RESULTS: Performance during the rugby passing task was improved following the positive video condition (91% [7.4%]) compared to the negative video (79% [6.0%]; ES: 1.22 ± 0.75) and self-visualization (86% [5.8%]; ES: 0.58 ± 0.75), with a significant correlation observed between passing performance and salivary testosterone (r = .47 ± .34, P = .0087). Positive video imagery prior to an exercise stressor also significantly enhanced physiological stress resilience (r = .39 ± .36, P = .0352). CONCLUSIONS: This pilot study demonstrates that mental rehearsal was enhanced by appropriate, context-specific video presentation. We propose that the interaction between sex steroids, the adrenal axis, and subsequent conscious and unconscious behaviors may be relevant to competitive rugby. Specifically, we suggest that relatively elevated free testosterone imparts a degree of stress resilience, which may lead to enhanced expression of competitive behaviors and provide an enhanced state for rugby skill execution.

Classifying Winning Performances in International Women's Rugby Union.

PURPOSE: The efficacy of isolated and relative performance indicators (PIs) has been compared in rugby union; the latter more effective at discerning match outcomes. However, this methodology has not been applied in women's rugby. The aim of this study was to identify PIs that maximize prediction accuracy of match outcome, from isolated and relative data sets, in women's rugby union. METHODS: Twenty-six PIs were selected from 110 women's international rugby matches between 2017 and 2022 to form an isolated data set, with relative data sets determined by subtracting corresponding opposition PIs. Random forest classification was completed on both data sets, and feature selection and importance were used to simplify models and interpret key PIs. Models were used in prediction on the 2021 World Cup to evaluate performance on unseen data. RESULTS: The isolated full model correctly classified 75% of outcomes (CI, 65%-82%), whereas the relative full model correctly classified 78% (CI, 69%-86%). Reduced respective models correctly classified 74% (CI, 65%-82%) and 76% (CI, 67%-84%). Reduced models correctly predicted 100% and 96% of outcomes for isolated and relative test data sets, respectively. No significant difference in accuracy was found between data sets. In the relative reduced model, meters made, clean breaks, missed tackles, lineouts lost, carries, and kicks from hand were significant. CONCLUSIONS: Increased relative meters made, clean breaks, carries, and kicks from hand and decreased relative missed tackles and lineouts lost were associated with success. This information can be utilized to inform physical and tactical preparation and direct physiological studies in women's rugby.

Performance Science Domains: Contemporary Strategies for Teams Preparing for the Rugby World Cup.

PURPOSE: As the start of the 10th Rugby Union World Cup approaches, performance staff will be working on the final elements of their teams' preparation. Much of this planning and preparation will be underpinned by the latest performance science research. In this invited commentary, we discuss contemporary performance science research in rugby union centered around 4 key performance domains. First, we outline a systematic approach to developing an overall understanding of the game demands and how performance staff can enhance the players' preparedness for competition. We then move on to outline our understanding of the training science domain, followed by a brief overview of effective recovery strategies at major tournaments. Finally, we outline research in the area of competition-day strategies and how they can positively impact players' readiness to compete. CONCLUSIONS: Evaluating a team's preparation for the Rugby Union World Cup can be achieved by mapping their performance plan based on the 4 domains outlined above.

Team Behavior and Performance: An Exploration in the Context of Professional Rugby Union.

PURPOSE: To explore complex system behavior and subsequent team performance in professional rugby union. METHODS: Here, we present 2 studies. In the first, we used global positioning system technology to measure player clustering during stoppages in play in nearly 100 games of professional rugby union to explore team (complex system) behavior and performance. In the second, we measured stress hormones (cortisol and testosterone) prior to team meetings and analyzed these relative to amount of time and the frequency with which players looked at peer presenters, as well as subsequent training performance, to explain how stress may lead to behaviors observed in the first study and subsequent match performance. RESULTS: No link between player clustering during stoppages of play and performance was observed. When players (complex system agents) demonstrated greater levels of stress (as indicated by greater cortisol-awakening response and a greater decline in testosterone-to-cortisol ratio across the morning), they tended to look at peer presenters more; however, training quality declined (P = .02). Correlational analysis also showed that training quality was related to testosterone-to-cortisol ratio (P = .04). CONCLUSIONS: Team behavior is complex and can be unpredictable. It is possible that under stress, complex system agents (ie, rugby union players) look at (and cluster toward) their teammates more; however, meaningful interaction may not necessarily occur. Furthermore, while complex system (team) analysis may be valuable strategically in rugby union in the context of describing behavior, without understanding "how" or "why" intrateam/interagent behaviors emerge it may have little meaning.

Day-to-day coordination of the stress and reproductive axes: A continuous-time analysis of within-person testosterone and cortisol relationships in athletic and healthy men.

Day-to-day coordination of the stress (i.e., hypothalamic-pituitary-adrenal [HPA]) and reproductive (i.e., hypothalamic-pituitary-gonadal [HPG]) axes is central to allostatic regulation, reproductive success, and survival. Reports of positive, within-person testosterone and cortisol relationships (or coupling) suggest cross-talk of a facilitative nature, but longitudinal evidence is scarce and has methodological and analytical limitations. To address this, we used a continuous-time (CT) model to investigate day-to-day, within-person coupling of testosterone and cortisol in two male cohorts. Salivary testosterone and cortisol fluctuations were monitored in 35 athletic men across two international tournaments (M = 19.3 tests) and in 41 healthy men during normal daily living (M = 27.9 tests). Bayesian CT analysis revealed a diminishing effect of each hormone on itself as time-interval length or lag increased. In both groups, cortisol had a negative lagged effect on testosterone that persisted for around three days. The cortisol effect on testosterone peaked after 0.71 and 0.51 days in athletic (standardized estimate = -0.13) and healthy men (standardized estimate = -0.11), respectively. Further estimates of non-lagged, contemporaneous correlations revealed positive testosterone and cortisol relationships (athlete r = 0.04, healthy r = 0.46). In summary, complex within-person HPA and HPG interplay emerged in two independent male cohorts. Specifically, a rising cortisol concentration was linked to a fall in testosterone concentration at later time points, but concurrently these hormones tended to rise and fall together. Our results suggest that inhibitory and facilitatory hormonal actions coexist on varying timescales, thereby expanding knowledge of HPG and HPA cross-talk in everyday life.

Exploring Finger Digit Ratios (2D:4D) in Surgeons, Professional Rugby Players, and Political Journalists to Form a Directional Hypothesis: Could Finger Length Predict Attention and Focus?

In this short report we explore the predictive nature of finger digit ratio (i.e., second/index finger length divided by fourth/ring finger length; 2D:4D) and achievement. This research, with niche and specialized populations, was intended to support and grow on knowledge obtained from other large population 2D:4D studies and help form a directional hypothesis for future work exploring finger digit ratio and "success." Twenty-nine professional rugby players aged 25.1 ± 4.2 years, height 185.2 ± 6.3 cm and weight 101.9 ± 11.8 kg; n = 16 orthopedic surgeons aged 55.3 ± 9.3 years with height 183.8 ± 10.2 cm and weight 90.8 ± 14.0 kg; and n = 18 political journalists with age, height and weight of 38.8 ± 7.3 years, 182.8 ± 7.8 cm, and 84.4 ± 11.4 kg, respectively, were recruited. Three experiments were conducted where we (1) explored relationships for 2D:4D with testosterone and cortisol responsiveness to low stress exercise, (2) explored relationships for 2D:4D with pupil constriction and pupil constriction latency (pupillometry measures related to testosterone and cortisol responsiveness and to attentiveness), and (3) compared 2D:4D between rugby players, surgeons, and journalists. Our results revealed 2D:4D was not predictive of testosterone and cortisol responsiveness to low-level exercise stress. However, relationships exist for 2D:4D and pupillometry measures (p < 0.05). Journalists right minus left 2D:4D difference was significantly different to rugby players' and surgeons (p < 0.05). We argue 2D:4D is likely predictive of testosterone sensitivity and associated ability to focus attention; a skill important to high achievement in various contexts.

The digit ratio (2D:4D) and testosterone co-predict vertical jump performance in athletic boys: Evidence of organizational and activational effects of testosterone on physical fitness.

The digit ratio (2D:4D) is a negative correlate of boy's physical fitness, and thought to arise from organizational effects of prenatal testosterone on different bodily systems. During human ontogeny, activational effects of testosterone on body size and strength offers another pathway to physical fitness. We tested these hypotheses by examining the organizational and activational effects of testosterone on vertical jump performance in athletic boys. Using a cross-sectional design, 173 boys (aged 9 to 18 years) were tested for standing height, body mass, body fat, fat-free mass, weekly training activity, training history, salivary testosterone and cortisol, R2D:4D, L2D:4D, and right-left 2D:4D (Dr-1), and vertical height in 3 different countermovement jump (CMJ) tests. A generalized additive model was employed to delineate age-related trajectories and predict CMJ performance. Our models yielded significant non-linear increases (or changes) in body size, current hormone concentration, training outcomes, and CMJ performance with chronological age. All 2D:4D measures were age invariant. The R2D:4D and testosterone were significant non-linear predictors of CMJ height with (R2 = 66.2%) or without (R2 = 54.3%) covariates, whereby a higher current testosterone concentration (up to a certain level) and a lower or higher R2D:4D were linked to better performance. The L2D:4D and Dr-1 had no predictive value. In conclusion, the R2D:4D and testosterone were co-predictors of CMJ height among athletic boys, with non-linear performance effects that differed in timing, tempo, and direction. Our findings confirm that testosterone can regulate a simple measure of boy's physical fitness through both an activational and organizational pathway.

Acute physiological and perceptual responses to a netball specific training session in professional female netball players.

The 24 h responses to professional female netball-specific training were examined. British Superleague players (n = 14) undertook a 90-min on-court training session incorporating key movement, technical, and scenario-specific match-play drills. Perceptual (mood, fatigue, soreness), neuromuscular (countermovement jump peak power output [PPO], PPO relative to mass [PPOrel], jump height [JH]), endocrine (salivary cortisol [C], testosterone [T] concentrations) and biochemical (creatine kinase concentrations [CK]) markers were assessed at baseline (immediately before; Pre), and immediately, two and 24 hours after (+0h, +2h, +24h) training. Session (sRPE) and differential (dRPE) ratings of perceived exertion were recorded at +0h. Identification of clear between time-point differences were based on the 95% confidence interval (CI) for mean differences relative to baseline values not overlapping. At +0h, C (raw unit mean difference from baseline; 95% CI: 0.16; 0.06 to 0.25 µg·dl-1), T (32; 20 to 45 pg⋅ml-1), CK (39; 28 to 50 u·L-1), PPOrel (2.4; 0.9 to 3.9 W·kg-1) and PPO (169; 52 to 286 W) increased. At +2h, fatigue (15; 7 to 24 AU), CK (49; 38 to 60 u·L-1), and soreness (14; 3 to 25 AU) increased, while T (-24; -37 to -11 pg⋅ml-1) and mood (-20; -27 to -12 AU) reduced. At +24h, CK increased (25; 13 to 36 u·L-1) whereas PPOrel (-1.6; -3.2 to -0.1 W·kg-1) and JH (-0.02; -0.03 to -0.08 m) reduced. Responses were variable specific, and recovery of all variables did not occur within 24h. The residual effects of the prior stimulus should be accounted for in the planning of training for professional female netball players.

Morning resistance exercise and cricket-specific repeated sprinting each improve indices of afternoon physical and cognitive performance in professional male cricketers.

OBJECTIVES: To compare two modes (general and cricket-specific) of morning priming exercise on afternoon physical and cognitive performance, and subjective readiness to perform in professional male cricketers. DESIGN: Randomised, crossover, counterbalanced. METHODS: On three occasions, 16 professional men's cricketers completed afternoon tests of countermovement jump height, cricket-specific sprint performance (running between the wickets, two runs), cognitive function (Stroop test, time taken), and subjective readiness to perform. Control (CON; passive rest), lower-body resistance exercise priming (LIFT; trap bar deadlifts, 6 × 4 repetitions up to 85% of one repetition maximum), or cricket-specific running priming (RUN; 6 × 35.36 m sprints including a 180° change of direction) interventions were implemented 5.5 h before testing. RESULTS: Afternoon sprint times were faster in RUN (-0.04 s, p = 0.013) and LIFT (-0.07 s, p < 0.001) versus CON, and faster in LIFT than RUN (-0.03 s, p = 0.032). Jump height (+1.1 cm, p = 0.021) and cognitive function (-3.83 s, p = 0.003) were greater in LIFT than CON, whilst RUN outperformed CON for cognition (-2.52 s, p = 0.023). Although perceived readiness was not influenced by trial (p > 0.05), players reported favourable responses on the "aggression" subscale in LIFT relative to CON (+1 arbitrary unit, p = 0.022). CONCLUSIONS: Both general (lower-body resistance exercise) and cricket-specific (simulated running between wickets) morning priming are effective match-day strategies to improve afternoon markers of physical and cognitive performance in professional men's cricketers. Practitioners may thus be afforded flexibility in situations where resistance exercise is not feasible on the morning of a match.

Impact of one HF-rTMS session over the DLPFC and motor cortex on acute hormone dynamics and emotional state in healthy adults: a sham-controlled pilot study.

Studies indicate that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) can lower cortisol concentration or output, with some evidence suggesting a link to testosterone. Together, these stress and social hormones might help regulate the emotional response to HF-rTMS. This pilot study evaluated the effect of HF-rTMS on acute testosterone and cortisol dynamics and emotional state in eleven healthy adults. Using a sham-controlled, single-blind, crossover design, participants completed a HF-rTMS session targeting the dorsolateral prefrontal cortex (DLPFC) and motor cortex on separate days. Stimulation (250 total pulses) was applied at 90% of the resting motor threshold. Salivary testosterone and cortisol, mood, motivation, anxiety, and heart rate (HR) were assessed before (T1) and 1 (T2), 15 (T3), and 30 min (T4) after each session. There were no significant session differences in testosterone and cortisol concentration, mood, motivation, and HR. Although DLPFC stimulation produced less anxiety (vs. motor cortex), and testosterone output was stable across both treatments (vs. sham-related decline in testosterone), neither differed from the sham. Within-person fluctuations in testosterone, mood, motivation, and/or anxiety were significantly related across the DLPFC and motor cortex trials only. In conclusion, a single sub-maximal session of HF-rTMS did not affect the hormonal, emotional, or physiological state of healthy adults, relative to a sham. However, the emergence of stimulation-specific testosterone and/or emotional linkages suggests that the repeated effects of HF-rTMS may also manifest at the individual level. This offers another pathway to explain the therapeutic efficacy of rTMS and a model to explore interindividual variability in health-related outcomes.

The neuromuscular, physiological, endocrine and perceptual responses to different training session orders in international female netball players.

AbstractThe 20 h responses of International female netball players to training days requiring two sessions (netball and strength, separated by two hours) ordered alternatively were examined. Eleven players completed strength followed by netball training two hours later (STR-NET), with the order reversed (NET-STR) on a separate day. Well-being, neuromuscular performance (jump height [JH], peak power output [PPO], peak velocity [PV]) and endocrine function (testosterone, cortisol concentrations) were measured before sessions one (PreS1) and two (PreS2), immediately after sessions one (IPS1) and two (IPS2), and 20 h post session one (20P). Session and differential ratings of perceived exertion (upper-body, cognitive/technical [RPE-T], lower-body, breathlessness) were collected, and accelerometry and heart rate measured netball load. Identification of clear between-order differences was based on the nonoverlap of the 95% confidence interval (95%CI) for mean differences relative to baseline. Compared to PreS1, greater increases in JH (percentage difference between trials; 95%CI: 9%; 4-14%), PPO (5%; 2-8%), PV (3%; 1-5%) and cortisol concentration (45%; 1-88%), and a greater decrease for testosterone/cortisol ratio (-35%; -72 to -2%) occurred at PreS2 in NET-STR. At 20P, greater decreases in JH (10%; 5-15%), PPO (4%; 1-8%) and PV (4%; 2-6%) were observed following STR-NET. No differences existed for well-being, whilst RPE-T was greater (15 AU; 3-26 AU) for strength training during NET-STR. Session order influenced neuromuscular and endocrine responses in International female netball players, highlighting session ordering as a key consideration when planning training.

Testosterone and Dihydrotestosterone Changes in Male and Female Athletes Relative to Training Status.

PURPOSE: To establish if training volume was associated with androgen baselines and androgen responsiveness to acute exercise. METHODS: During a "high-volume" training phase, 28 cyclists (14 men and 14 women) undertook oxygen-uptake and maximal-work-capacity testing. Two days later, they completed a repeat-sprint protocol, which was repeated 3 weeks later during a "low-volume" phase. Blood and saliva samples were collected before and after (+5 and +60 min) the repeat-sprint protocol. Blood was assayed for total testosterone (TT), free testosterone (FT), and dihydrotestosterone (DHT) and saliva, for testosterone and DHT. RESULTS: Pretrial TT, FT, and DHT concentration was greater for males (P < .001, large effect size differences), and in both genders TT, DHT, and saliva for DHT was higher during high-volume loading (moderate to large effect size). Area-under-the-curve analysis revealed larger TT, FT, and DHT responses to the repeat-sprint protocol among females, and high-volume training was linked to larger TT, DHT, and saliva for DHT responses (moderate to large effect size). Baseline TT and FT correlated with oxygen uptake and work capacity in both genders (P < .05). CONCLUSION: DHT showed no acute performance correlation but was responsive to volume of training, particularly in females. This work informs on timelines and relationships of androgenic biomarkers in males and females across different training loads, adding to the complexity that should be considered in interpretation thereof. The authors speculate that testosterone may impact acute performance via behavioral mechanisms of motivation and attention; DHT, via training volume-induced androgenic promotion, may facilitate long-term adaptive changes, especially for females.

Testosterone and cortisol are more predictive of choice behavior than a social nudge in adult males on a simple gift give-get task.

Reproducibility of social research is ambitious, and evidence supporting this argument is increasing in psychology and social science research. This may be attributed to, in part, the high volume of qualitative research methodology used in social research along with difficulties in the reliability of measurement techniques. Therefore, use of more and better objective measures to complement existing techniques in social research are necessitated. To highlight this point we explored the success of give-get nudge in adults. Nudge being a subtle intervention to influence choice, without restricting choice. We also wanted to explore whether testosterone and cortisol, as objective psychophysiological markers, could explain nudge outcome. Participants were asked what they would like to get for Christmas, or what they would like to give. They were then presented with two chocolates, one big and one small, and instructed to take as a "reward" for their participation with the knowledge there was one other participant to take chocolate after them. It was hypothesized that those asked to give something for Christmas would take the smaller reward and vice versa. Salivary testosterone and cortisol were measured prior to, and 10 min after completing the exercise. The nature of the nudge itself did not predict behavior, but the hormone measures did. We speculate that testosterone may focus an individual on the nature of the question (nudge), while cortisol encourages self-focus. These results support the need to combine existing social research techniques with more objective markers.

Menstrual variation in the acute testosterone and cortisol response to laboratory stressors correlate with baseline testosterone fluctuations at a within- and between-person level.

Studies have compared HPA and HPG stress reactivity across the follicular and luteal phases to assess the menstrual impact of estradiol and progesterone fluctuations. Ovulatory shifts in baseline and stressor-induced testosterone among athletic women offer a new framework to explore these responses. Here we investigated menstrual variation in baseline testosterone as a predictor of the acute testosterone and cortisol response to laboratory stressors in female athletes. Using a semi-randomized crossover design, thirty athletic women completed a physical (4 × 6-s bike sprints) and psychological (5 × 2-min cognitive tests with social evaluation) stressor on day seven (D7), 14 (D14), and 21 (D21) of a menstrual cycle. Baseline fluctuations and acute changes in salivary testosterone and cortisol were measured. The D14 testosterone response to both stressors (13.7%) exceeded D7 (7.3%) and D21 (7.0%), whereas cortisol was less responsive on D14 (9.8%) than D7 (13.0%) and D21 (12.0%); all moderate to large effect size differences (p < 0.01). Baseline testosterone, which presented large individual and menstrual variation with a D14 peak, was significantly related (moderate correlations) to testosterone and cortisol stress reactivity on a between-person level. Both outcomes were related (weak correlations) to within-person fluctuations in baseline testosterone, but these effects were mediated by testing day. In conclusion, menstrual variation in baseline testosterone concentration correlated with testosterone and cortisol reactivity to a physical and psychological stressor. Thus, gradients of stressor-induced hormonal change showed some dependency to endogenous testosterone, both individual differences and fluctuations over time, among naturally cycling athletic women.Lay summaryThe female menstrual cycle is accompanied by dramatic shifts in estradiol and progesterone concentration, but less is known about testosterone variability and its role in stress regulation. In this study, menstrual fluctuations in baseline testosterone concentration correlated with acute testosterone and cortisol reactivity to laboratory stressors.

The impact of menstrual-cycle phase on basal and exercise-induced hormones, mood, anxiety and exercise performance in physically active women.

BACKGROUND: The influence of menstrual cycle phase on perceptual responses and exercise performance is still unclear in the literature. Therefore, this study investigated salivary estradiol (sal-E2) and cortisol (sal-C) concentrations, mood, anxiety and exercise (aerobic, anaerobic) performance in physically-active women across two menstrual-cycle phases. METHODS: Twelve women (mean age 24.9±4.3 years) were assessed in the early follicular (early-FP) and mid luteal (mid-LP) phase of their menstrual cycle. In each phase, participants were tested for both aerobic (i.e. VO2max) and anaerobic (i.e. peak power, average power and Fatigue Index) performance. Basal and exercise-induced changes in sal-E2 and sal-C concentrations, self-appraised mood and anxiety were assessed. RESULTS: We observed a significant increase in basal (pre-exercise) sal-E2 concentration from early-FP to mid-LP (P≤0.05), coupled with a significant increase in VO2max in early-FP (39.9±7.8 mL/kg/min) versus mid-LP (36.9±7.8 mL/kg/min). Depression also decreased with aerobic exercise, but only in the early-FP. No other significant menstrual-phase differences in exercise performance, emotional state or hormonal change scores were identified. CONCLUSIONS: Our data suggest that physically-active women may experience a natural rise in estradiol concentration, as they transition from the early-FP to mid-LP. In the present study, this was accompanied by a small reduction in VO2max. An exercise (aerobic)-related decline in depression also emerged in the early-FP. Most of the exercise performance, emotional state and hormonal measures did not exhibit any menstrual phase-related difference.

Effect of Ischemic Preconditioning on Maximal Swimming Performance.

ABSTRACT: Williams, N, Russell, M, Cook, CJ, and Kilduff, LP. Effect of ischemic preconditioning on maximal swimming performance. J Strength Cond Res 35(1): 221-226, 2021-The effect of ischemic preconditioning (IPC) on swimming performance was examined. Using a randomized, crossover design, national- and international-level swimmers (n = 20; 14 men, 6 women) participated in 3 trials (Con, IPC-2h, and IPC-24h). Lower-body IPC (4 × 5-minute bilateral blood flow restriction at 160-228 mm Hg and 5-minute reperfusion) was used 2 hours (IPC-2h) or 24 hours (IPC-24h) before a self-selected (100 m, n = 15; 200 m, n = 5) swimming time trial (TT). The Con trial used a sham intervention (15 mm Hg) 2 hours before exercise. All trials required a 40-minute standardized precompetition swimming warm-up (followed by 20-minute rest; replicating precompetition call room procedures) 1 hour before TT. Capillary blood (pH, blood gases, and lactate concentrations) was taken immediately before and after IPC, before TT and after TT. No effects on TT for 100 m (P = 0.995; IPC-2h: 64.94 ± 8.33 seconds; IPC-24h: 64.67 ± 8.50 seconds; Con: 64.94 ± 8.24 seconds), 200 m (P = 0.405; IPC-2h: 127.70 ± 10.66 seconds; IPC-24h: 129.26 ± 12.99 seconds; Con: 130.19 ± 10.27 seconds), or combined total time (IPC-2h: 84.27 ± 31.52 seconds; IPC-24h: 79.87 ± 29.72 seconds; Con: 80.55 ± 31.35 seconds) were observed after IPC. Base excess (IPC-2h: -13.37 ± 8.90 mmol·L-1; Con: -13.35 ± 7.07 mmol·L-1; IPC-24h: -16.53 ± 4.65 mmol·L-1), pH (0.22 ± 0.08; all conditions), bicarbonate (IPC-2h: -11.66 ± 3.52 mmol·L-1; Con: -11.62 ± 5.59 mmol·L-1; IPC-24h: -8.47 ± 9.02 mmol·L-1), total carbon dioxide (IPC-2h: -12.90 ± 3.92 mmol·L-1; Con: -11.55 ± 7.61 mmol·L-1; IPC-24h: 9.90 ± 8.40 mmol·L-1), percentage oxygen saturation (IPC-2h: -0.16 ± 1.86%; Con: +0.20 ± 1.93%; IPC-24h: +0.47 ± 2.10%), and blood lactate (IPC-2h: +12.87 ± 3.62 mmol·L-1; Con: +12.41 ± 4.02 mmol·L-1; IPC-24h: +13.27 ± 3.81 mmol·L-1) were influenced by swimming TT (P < 0.001), but not condition (all P > 0.05). No effect of IPC was seen when applied 2 or 24 hours before swimming TT on any indices of performance or physiological measures recorded.