1.
Appl Environ Microbiol
; 60(8): 3023-6, 1994 Aug.
Article
in English
| MEDLINE
| ID: mdl-8085838
ABSTRACT
The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.