Eimeria gilruthi-associated abomasitis in a group of ewes.

Nine of 23 (39%) research ewes with severe diarrhea and weight loss had histologic lesions consistent with Eimeria gilruthi infection in their abomasa. Gross anatomic lesions included hundreds of opaque 1-mm nodules in abomasal mucosa that corresponded microscopically to 200-300 µm diameter organisms surrounded by areas of necrosis. Analysis of fecal samples from 4 ewes demonstrated oocysts from typical ovine Eimeria species, none of which were E. gilruthi. Two separate PCR reactions were performed on abomasal tissue from 4 sheep to amplify the 18S ribosomal DNA (rDNA) and internal transcribed spacer (ITS) region of the rDNA, respectively. The resultant 18S rDNA nucleotide sequences shared 99% homology with multiple Eimeria species in GenBank. The ITS region shared 77% homology with E. ellipsoidalis in GenBank. Further studies are needed to understand the life cycle and pathogenicity of E. gilruthi. Our results underscore the inclusion of E. gilruthi in the differential diagnosis of diarrhea and weight loss in sheep.

Winter grazing of stockpiled native forages during heifer development delays body weight gain without influencing final pregnancy rates.

The objective of this study was to test the effects of protein supplementation strategy and different stockpiled forage species on growth, nutritional status, and reproductive performance of yearling beef heifers. In a 5-yr study, yearling beef heifers (n = 266) were stratified by body weight (BW) at weaning to 1 of 3 stockpiled forages: 1) endophyte-infected tall fescue (TF, Schedonorus arundinaceus (Schreb.) Dumort; 7.21% crude protein [CP] and 67.13% neutral detergent fiber [NDF], dry matter [DM] basis), 2) big bluestem (Andropogon gerardi Vitman) and indiangrass (Sorghastrum nutans L.) combination (BI; 4.32% CP and 71.06% NDF, DM basis), or 3) switchgrass (SG,Panicum virgatum L.; 3.87% CP and 76.79% NDF, DM basis). Forage treatments were then randomly assigned to receive 1 of 2 supplement types: 1) 0.68 kg heifer-1 d-1 of dried distillers grains with solubles (DDGS: 28% CP and 108% total digestible nutrients [TDN]) or 2) 0.22 kg heifer-1 d-1 of blood meal and fish meal (BF: 72.5% CP and 77.5% TDN), resulting in a 3 × 2 factorial arrangement of treatments. Each year, twenty-one 1.2-ha pastures (7 pastures per forage type) were utilized with 2 to 3 heifers per pastures. Treatments were initiated in January and terminated in April at the initiation of breeding. Initial BW was not different (P ≥ 0.22) by forage or supplement type. During the rest of the grazing period, BW was greater (P < 0.01) for TF heifers. However, average daily gain (ADG) was greater (P < 0.01) for BI and SG heifers from breeding to final pregnancy diagnosis. Heifers grazing TF pastures had greater (P < 0.01) overall ADG than their counterparts. The percentage of mature BW (MBW) at breeding was greater (P < 0.01) for TF heifers. Heifer BW and ADG was not influenced (P ≥ 0.06) by supplementation strategy. Serum glucose concentrations were not different (P ≥ 0.44) among forage type or supplement strategy. Pregnancy rates at fixed timed-artificial insemination and overall pregnancy rates did not differ (P ≥ 0.38) by forage or supplement treatment. Owing to forage nutritive value differences, heifers grazing low-quality, warm season grasses lost BW prior to the initiation of the breeding season. However, a negative BW gain prior to breeding did not negatively impact overall pregnancy rates.

Beta-hydroxybutyrate infusion identifies acutely differentially expressed genes related to metabolism and reproduction in the hypothalamus and pituitary of castrated male sheep.

To identify molecular pathways that couple metabolic imbalances and reproduction, we randomly assigned 10 castrated male sheep to be centrally injected into the lateral ventricle through intracerebroventricular cannulas with 1 ml of ß-hydroxybutyric acid sodium salt solution (BHB; 12,800 µmol/l) or saline solution (CON; 0.9% NaCl). Approximately 2 h postinjection, sheep were humanely euthanized, and hypothalamus and pituitary tissues were harvested for transcriptome characterization by RNA sequencing. RNA was extracted from the hypothalamus and pituitary and sequenced at a high depth (hypothalamus: 468,912,732 reads; pituitary: 515,106,092 reads) with the Illumina Hi-Seq 2500 platform and aligned to Bos taurus and Ovis aries genomes. Of the total raw reads, 87% (hypothalamus) and 90.5% (pituitary) mapped to the reference O. aries genome. Within these read sets, ~56% in hypothalamus and 69% in pituitary mapped to either known or putative protein coding genes. Fragments per kilobase of transcripts per million normalized counts were averaged and ranked to identify the transcript expression level. Gene Ontology analysis (DAVID Bioinformatics Resources) was utilized to identify biological process functions related to genes shared between tissues, as well as functional categories with tissue-specific enrichment. Between CON- and BHB-treated sheep, 11 and 44 genes were differentially expressed (adj. P < 0.05) within the pituitary and hypothalamus, respectively. Functional enrichment analyses revealed BHB altered expression of genes in pathways related to stimulus perception, inflammation, and cell cycle control. The set of genes altered by BHB creates a foundation from which to identify the signaling pathways that impact reproduction during metabolic imbalances.