Temperature responses of the Rubisco maximum carboxylase activity across domains of life: phylogenetic signals, trade-offs, and importance for carbon gain.

Temperature response of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalytic properties directly determines the CO2 assimilation capacity of photosynthetic organisms as well as their survival in environments with different thermal conditions. Despite unquestionable importance of Rubisco, the comprehensive analysis summarizing temperature responses of Rubisco traits across lineages of carbon-fixing organisms is lacking. Here, we present a review of the temperature responses of Rubisco carboxylase specific activity (c(cat)(c)) within and across domains of life. In particular, we consider the variability of temperature responses, and their ecological, physiological, and evolutionary controls. We observed over two-fold differences in the energy of activation (ΔH(a)) among different groups of photosynthetic organisms, and found significant differences between C3 plants from cool habitats, C3 plants from warm habitats and C4 plants. According to phylogenetically independent contrast analysis, ΔH(a) was not related to the species optimum growth temperature (T growth), but was positively correlated with Rubisco specificity factor (S(c/o)) across all organisms. However, when only land plants were analyzed, ΔH(a) was positively correlated with both T(growth) and S(c/o), indicating different trends for these traits in plants versus unicellular aquatic organisms, such as algae and bacteria. The optimum temperature (T(opt)) for k(cat)(c) correlated with S(c/o) for land plants and for all organisms pooled, but the effect of T growth on T(opt) was driven by species phylogeny. The overall phylogenetic signal was significant for all analyzed parameters, stressing the importance of considering the evolutionary framework and accounting for shared ancestry when deciphering relationships between Rubisco kinetic parameters. We argue that these findings have important implications for improving global photosynthesis models.

Foliar limonene uptake scales positively with leaf lipid content: "non-emitting" species absorb and release monoterpenes.

Monoterpenes synthesized and released by emitting vegetation can be taken up by neighboring non-emitting plants, but the uptake capacity of non-emitting species has not been studied extensively. We investigated the foliar uptake potential of the hydrophobic monoterpene limonene in 13 species of contrasting leaf structure and lipid content to determine the structural and chemical controls of monoterpene uptake. Leaf dry mass per unit area (M(A,D)) varied 6.5-fold, dry to fresh mass ratio (D(F)) 2.7-fold, lipid content per dry mass (L(M)) 2.5-fold and per unit area (L(A)) 4.6-fold across the studied species. Average foliar limonene uptake rate (U(A)) from air at saturating limonene partial pressures varied from 0.9 to 6 nmol m(-2) s(-1), and limonene leaf to air partition coefficient (K(FA), ratio of limonene content per dry mass to limonene partial pressure) from 0.7 to 6.8 micromol kg(-1) Pa(-1). U(A) and K(FA) scaled positively with leaf lipid content, and were independent of D(F), indicating that variation in leaf lipid content was the primary determinant of species differences in monoterpene uptake rate and K(FA). Mass-based limonene uptake rates further suggested that thinner leaves with greater surface area per unit dry mass have higher uptake rates. In addition, limonene lipid to air partition coefficient (K(LA)=K(FA)/L(M)) varied 19-fold, indicating large differences in limonene uptake capacity at common leaf lipid content. We suggest that the significant uptake of hydrophobic monoterpenes when monoterpene ambient air concentration is high and release when the concentration is low should be included in large-scale monoterpene emission models.