ABSTRACT
The increase of oxygen consumption in liver cell cultures in medium 199 with addition of serum or of regenerative liver extract pleads for the existence of a factor stimulating energy-dependent processes (mitoses, protein synthesis), which is active both in vivo and in vitro. This stimulating factor is of liver origin, but with systemic offer. The concentration of the stimulating factor, which is the highest 3 days after operation, decreases as the normal liver cell population is re-established. The stimulating factor shows a limited specificity for tissues with epithelial structure and in vivo increased mitotic index the leukocytes being reactive only to very high concentrations of this factor. An optimal concentration of the stimulating factor exists which induces the more marked increase of oxygen consumption.
Subject(s)
Liver/metabolism , Oxygen Consumption , Animals , Cells, Cultured , Female , Hepatectomy , Liver/cytology , Liver/drug effects , Liver Regeneration/drug effects , Male , Oxygen Consumption/drug effects , Rats , Rats, Inbred Strains , Tissue Extracts/pharmacologyABSTRACT
Subtotal hepatectomy and all the paraoperative actions induce a condition of stress characterized by the intensification of catabolic processes and overproduction of hormones with glycogenolytic and lypolytic action: catecholamines, T3, T4, ensuring the conditions required by some energy-dependent processes (mitoses and proteosyntheses) assuring the regeneration of the operated liver. The latter presents a depletion of dehydrogenases, of PAS + materials and fatty loading of the liver cells. In the conditions of liver distress, the muscles are those which supply the glucose, ensuring an as rapid as possible return of glycemia to normal values.
Subject(s)
Liver/physiology , Animals , Catecholamines/blood , Female , Hepatectomy , Histocytochemistry , Insulin/blood , Liver Glycogen/metabolism , Liver Regeneration , Male , Postoperative Period , Rats , Rats, Inbred Strains , Thyroxine/blood , Time Factors , Triiodothyronine/bloodABSTRACT
Partial hepatectomy led to an increased oxygen consumption over the whole respiratory cycle. Association of partial hepatectomy with negative aeroionotherapy determined a decrease of oxygen consumption as compared with the partially hepatectomized animals. Negative air ionization in controls determined a decrease of oxygen consumption at the level of the NAD+ dependent enzymes. On the other hand, neither partial hepatectomy, nor negative air ionization altered the oxidative phosphorylation of mitochondria.
