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Biochimie ; 58(3): 297-304, 1976.
Article in French | MEDLINE | ID: mdl-1276237

ABSTRACT

The hydrophobic chromatography on alkylated Sepharose allows to separate the histones into three groups which exhibit an increasing affinity for the support HI less than H2A-H2B less than H3-H4. In this fractionation procedure, the behaviour of the histones taken separately or pair-associated, is discussed in relation with the ability of these proteins to complex each other in vitro and in vivo.


Subject(s)
Histones/isolation & purification , Adsorption , Alkylation , Animals , Cattle , Chromatography, Affinity , Chromatography, Agarose , Osmolar Concentration , Protein Binding , Protein Conformation
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