ABSTRACT
INTRODUCTION: The aim of the ISAAC 1 study was to determine the prevalence and severity of asthma among adolescents in Guadeloupe. MATERIALS AND METHODS: The study was carried out with a representative sample of young people in the second and third years of "college" (secondary school) in classes selected at random. The anonymous questionnaires were completed by pupils in 2003. 5097 questionnaires were processed. RESULTS: 49.7% of the adolescents stated they had already felt breathless or oppressed; 29% said they had experienced wheezing. Of the 812 adolescents who declared they had already suffered asthma attacks the medical diagnosis was confirmed for 89%. 22% had been hospitalised at least once for asthma. A high proportion had suffered severe attacks (3.5%). A considerable number of school days had been lost by asthma sufferers. Medical care is inappropriate and the pupils' knowledge of the peak flow is poor (only 15%). CONCLUSIONS: The study confirmed the high frequency of asthma in Guadeloupe (14.1%) with high indicators of severity and it allowed a comparison to be made between Guadeloupe and the other regions which have carried out the ISAAC 1 study.
Subject(s)
Asthma/epidemiology , Cross-Cultural Comparison , Absenteeism , Adolescent , Asthma/diagnosis , Asthma/etiology , Asthma/therapy , Child , Cross-Sectional Studies , Disease Progression , Female , Guadeloupe , Health Surveys , Hospitalization/statistics & numerical data , Humans , Incidence , Male , Respiratory Hypersensitivity/diagnosis , Respiratory Hypersensitivity/epidemiology , Respiratory Hypersensitivity/etiology , Respiratory Hypersensitivity/therapy , Surveys and Questionnaires , Treatment OutcomeABSTRACT
Mitochondrial proteins from rat brain cortex, muscle, liver, and from neuronal cells in culture were compared on 2-D electrophoregrams. This analysis permitted characterization of certain specificities in the distribution of polypeptides depending on tissue localization. In particular, 16 mit-proteins were found exclusively in the mitochondrion from brain tissue.
Subject(s)
Cerebral Cortex/analysis , Mitochondria/analysis , Neurons/analysis , Proteins/analysis , Animals , Cells, Cultured , Electrophoresis , Male , Mitochondria, Liver/analysis , Mitochondria, Muscle/analysis , Rats , Rats, Inbred StrainsABSTRACT
The effects of peripheral benzodiazepines on the respiration of a neuronal cell, the mouse C 1300 neuroblastoma, were analyzed. The presence of 'peripheral receptors' to the [3H]PK 11195 ligand was checked in these cells. A dose-dependent decrease of the O2 consumption in the presence of Ro 5-4864 and PK 11195 was observed at concentrations consistent with a receptor-mediated action. Diazepam, clonazepam and Ro 15-1788 were inactive. Previous studies have localized the peripheral benzodiazepine receptors on the mitochondrial outer membrane. We report here an effect of the peripheral ligand on mitochondrial metabolism.
