ABSTRACT
Equine infectious anemia is a worldwide distributed disease that affects the Equide family. Commercial effective vaccine is not available, for that reason control of the disease depends on diagnostic tools. To improve the efficiency of the diagnostic program in Cuba, LABIOFAM Group, developed an indirect enzyme-linked immunosorbent assay (ELISA), ELISA kit, to complement the diagnostic system that currently uses the agar gel immunodiffusion (AGID) kit. The ELISA AIE-LAB Kit was evaluated in a Mexican context, compared with the gold standard test Agar gel immunodiffusion, AGID AIE-LABIOFAM, and commercial AGID kit. The analytical sensitivity was determined using serial dilutions twofold of the positive control serum to establish the range of detected antibodies in relation to the cutoff value of the plate (OD 0.300). A precision study was carried out to evaluate repeatability, intermediate precision, and reproducibility by estimating the standard deviation and coefficient of variation. The precision results were satisfactory and the values of the coefficient of variation were considered adequate to guarantee an excellent consistency of the ELISA AIE-LAB. The diagnostic performance of the ELISA AIE-LAB involved the evaluation of specificity, sensitivity, and concordance in comparison with both AGID tests. The diagnostic sensitivity was 100% and the specificity 97.6%, with a very good degree of concordance (Kappa = 0.9). The results suggest that the ELISA AIE-LAB test could be used in Mexico as a diagnostic system for the detection of specific antibodies against the equine infectious anemia virus, as per current international norms.
Subject(s)
Equine Infectious Anemia , Horse Diseases , Infectious Anemia Virus, Equine , Animals , Antibodies, Viral , Cuba , Enzyme-Linked Immunosorbent Assay/veterinary , Equine Infectious Anemia/diagnosis , Horses , Mexico , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
La peste porcina clásica (PPC) es una enfermedad viral infectocontagiosa, producida por un virus ARN del género Pestivirus, familia Flaviviridae. En la actualidad es una de las causas de pérdidas económicas en la industria porcina a nivel mundial. En su prevención se han utilizado vacunas vivas atenuadas, empleando la cepa China lapinizada. La Reacción en Cadena de la Polimerasa Reverso Transcriptasa (RT-PCR) ha sido uno de los métodos más sensibles aplicado en Medicina Veterinaria para la detección de virus ARN. En el caso del virus de la PPC es muy útil porque el ácido nucleico se puede detectar desde muy temprano en la infección y en periodos más largos en aquellos animales que se recuperan. El objetivo de este estudio fue aplicar la técnica de RT-PCR en tiempo real para la detección de la cepa China lapinizada de la vacuna cubana contra la PPC. Las tonsilas de los cerdos vacunados fueron el órgano más positivo en la detección del ARN del virus vacunal. Los resultados obtenidos evidenciaron una interferencia del virus vacunal en el diagnóstico, siendo el día 12 posvacunación en el que se obtiene una emisión umbral de fluorescencia más bajo(AU)
Classical swine fever (CSF) is a highly contagious viral disease caused by a RNA virus that belongs to the genus Pestivirus within the family Flaviviridae. CSF represents one of the leading worldwide threats to the pig industry. Life attenuated vaccines using lapinized Chinese strain have been used to prevent the disease. The reverse transcriptase polymerase chain reaction (RT-PCR) is one of the more sensitive methods that have been used in veterinary medicine for RNA virus detection. In the case of CSF virus is very useful, because the nucleic acid could be detected in early stages of the infection and during a long period in recovered animals. The aim of this study was to apply real time RT-PCR assay for the detection of lapinized Chinese strain from the Cuban vaccine strain against CSF. Tonsils of vaccinated pigs were the most positive organ in the detection of RNA vaccinal virus. Interference of the vaccinal virus in diagnosis was proved. The lower threshold cycle values were seen at 12 postvaccination day(AU)
