Identification of BRAF V600E mutation in odontogenic tumors by high-performance MALDI-TOF analysis / 国际口腔科学杂志·英文版

Odontogenic tumors are rare lesions with unknown etiopathogenesis. Most of them are benign, but local aggressiveness, infiltrative potential, and high recurrence rate characterize some entities. The MAP-kinase pathway activation can represent a primary critical event in odontogenic tumorigenesis. Especially, the BRAF V600E mutation has been involved in 80-90% of ameloblastic lesions, offering a biological rationale for developing new targeted therapies. The study aims to evaluate the BRAF V600E mutation in odontogenic lesions, comparing three different detection methods and focusing on the Sequenom MassARRAY System. 81 surgical samples of odontogenic lesions were subjected to immunohistochemical analysis, Sanger Sequencing, and Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (Sequenom). The BRAF V600E mutation was revealed only in ameloblastoma samples. Moreover, the presence of BRAF V600E was significantly associated with the mandibular site (ρ = 0.627; P value <0.001) and the unicystic histotype (ρ = 0.299, P value <0.001). However, any significant difference of 10-years disease-free survival time was not revealed. Finally, Sequenom showed to be a 100% sensitive and 98.1% specific, suggesting its high-performance diagnostic accuracy. These results suggest the MAP-kinase pathway could contribute to ameloblastic tumorigenesis. Moreover, they could indicate the anatomical specificity of the driving mutations of mandibular ameloblastomas, providing a biological rational for developing new targeted therapies. Finally, the high diagnostic accuracy of Sequenom was confirmed.

Human maxillary sinuses augmented with mineralized, solvent-dehydrated bone allograft: a longitudinal case series.

BACKGROUND: This prospective case series aimed to assess the clinical stability of maxillary sinus floors regenerated with mineralized, solvent-dehydrated bone allograft (MSDBA) and restored with dental implants after at least 5 years of function. MATERIALS AND METHODS: Eleven sinuses in 7 patients (4 bilateral) were augmented with 50% cortical and 50% cancellous MSDBA (Puros, Zimmer Dental Italy srl, Vittorio Veneto, Treviso, Italy). After a mean healing period of 8.1 months, a total of 11 bone specimens (1 per sinus graft) were retrieved and 25 implants were placed. Bone samples were analyzed using histology, histomorphometry, and immunohistochemistry. Standardized periapical radiographs were taken at prosthetic loading (baseline) and annually. RESULTS: There were no complications or failures. Histologically, almost all residual MSDBA particles were surrounded by bone. Histomorphometry characterized the tissue as 39% ± 1.6% newly formed bone, 34% ± 1.6% marrow spaces, and 31% ± 1.4% residual material reflective of high cortical content of the composite graft. Immunohistochemistry indicated that osteoblasts expressed OB-cadherin at high levels. After a mean follow-up of 5.1 years, implants and regenerated bone appeared clinically stable. Radiographically, implants appeared to be surrounded by dense bone and ridges did not lose clinically measurable volume. CONCLUSIONS: Composite MSDBA allografts formed good quality maxillary sinus bone without complications after 8.1 months of mean healing time, and demonstrated clinical and radiographic stability after a mean follow-up time of 5.14 years.