ABSTRACT
CASE REPORT: We describe the unusual diagnosis of a ciliary body medulloepithelioma by histopathology of a subretinal membrane obtained from vitreoretinal surgery of a 10-year-old boy. The patient had a history of perforating trauma OS 2 years earlier, and both fundus exam and B-scan ultrasound revealed only a retinal detachment with a subretinal membrane. No detectable mass was present. DISCUSSION: The membrane removed from underneath the peripheral retina revealed a blue cell tumor confirmed by histopathology and immunohistochemistry to be a primitive neuroectodermal tumor. Currently, the patient has been followed for 5 years with no signs of recurrence.
Subject(s)
Ciliary Body/pathology , Eye Injuries, Penetrating/complications , Neuroectodermal Tumors, Primitive/complications , Uveal Neoplasms/complications , Cataract/etiology , Child , Ciliary Body/surgery , Diagnosis, Differential , Eye Injuries, Penetrating/diagnostic imaging , False Negative Reactions , Follow-Up Studies , Humans , Light Coagulation , Male , Neuroectodermal Tumors, Primitive/diagnosis , Neuroectodermal Tumors, Primitive/pathology , Neuroectodermal Tumors, Primitive/surgery , Remission Induction , Retinal Detachment/diagnostic imaging , Retinal Detachment/etiology , Retinoblastoma/diagnosis , Time Factors , Tomography, X-Ray Computed , Ultrasonography , Uveal Neoplasms/diagnosis , Uveal Neoplasms/pathology , Uveal Neoplasms/surgery , VitrectomyABSTRACT
PURPOSE: To examine the effect of nepafenac, a selective cyclooxygenase-2 (COX-2) inhibitor, on the proliferation rate of two human retinoblastoma (Rb)cell lines. METHODS: Two human Rb cell lines (WERI-RB and Y79) were cultured. COX-2 expression in these cell lines was verified by immunocytochemical analysis of cytospin sections and Western blotting. An MTT-based proliferation assay was used to compare Rb cell growth with and without amfenac, the active metabolite of nepafenac. The averaged results per condition were recorded. The Student's t-test was used to compare results from the cells cultured with and without amfenac. RESULT: The Y79 cell line showed a higher proliferative rate than the WERI-RB cell line. Both cell lines were negative for COX-2 expression by immunocytochemical analysis; however, both cell lines were positive for COX-2 expression by Western blot. When amfenac was added to both of the cell lines, a statistically significant reduction in proliferation was observed in both cell lines. The two Rb cell lines were positive for COX-2 only in the Western blot, indicating that they probably express low levels of COX-2, which was undetectable by immunocytochemical analysis. CONCLUSION: The selective, anti-COX-2 molecule amfenac inhibited proliferation of both tested Rb cell lines. Further trials should be undertaken to study the effect of selective COX-2 inhibitors on Rb.
Subject(s)
Cyclooxygenase 2 Inhibitors/pharmacology , Phenylacetates/pharmacology , Retinal Neoplasms/pathology , Retinoblastoma/pathology , Blotting, Western , Cell Proliferation/drug effects , Cyclooxygenase 2/metabolism , Humans , Retinal Neoplasms/enzymology , Retinoblastoma/enzymology , Tumor Cells, CulturedABSTRACT
PURPOSE: The management of retinoblastoma has been shifting toward conservative treatment. Visual preservation has become a concern as tumor regression is achieved. To report the surgical approach and outcome of a radiation-induced cataract in an only eye with regressed retinoblastoma. METHODS: Single case report and review of literature. RESULTS: Small incision sutureless phacoemulsification and implant of an acrylic foldable intraocular lens was performed. Best-corrected visual acuity 1 year after surgery was 20/20. No tumor recurrence has been noted after 27 months of follow-up. CONCLUSIONS: Phacoemulsification and foldable intraocular lens implant seemed to be an appropriate therapeutic option for this case.
Subject(s)
Cataract/etiology , Lens Implantation, Intraocular/methods , Lens, Crystalline/radiation effects , Phacoemulsification/methods , Radiation Injuries/etiology , Retinal Neoplasms/radiotherapy , Retinoblastoma/radiotherapy , Child, Preschool , Humans , Lenses, Intraocular , Male , Radiotherapy/adverse effects , Remission Induction , Treatment OutcomeABSTRACT
PURPOSE: The management of retinoblastoma has been shifting toward conservative treatment. Visual preservation has become a concern as tumor re g ression is achieved. To report the surgical approach and outcome of a radiation-induced cataract in an only eye with regressed retinoblastoma. METHODS: Single case report and review of literature. RESULTS: Small incision sutureless phacoemulsification and implant of an acrylic foldable intraocular lens was performed. Best-corrected visual acuity 1 year after surgery was 20/20. No tumor recurrence has been noted after 27 months of follow-up. CONCLUSIONS: Phacoemulsification and foldable iintraocular lens implant seemed to be an appropriate therapeutic option for this case.
