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Expression of the Canavalia brasiliensis lectin (ConBr) in tobacco plants.
da Silveira Carvalho, Cristina Paiva; Correia, Tuana Oliveira; Ribeiro, Sandra Mara Serafim; Silva, Ingrid Cavalcante; de Oliveira, José Tadeu Abreu; Grangeiro, Thalles Barbosa.
Protein Pept Lett ; 13(10): 1045-9, 2006.
Article in English | MEDLINE | ID: mdl-17168828

ABSTRACT

Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.


Subject(s)
Canavalia/genetics , Nicotiana/genetics , Plant Lectins/biosynthesis , Plant Lectins/genetics , Animals , Cloning, Molecular , Plant Leaves/genetics , Plants, Genetically Modified , Rabbits
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