ABSTRACT
A prevalencia da infeccao pelo Trypanosoma cruzi foi pesquisada em Oitis, uma pequena comunidade do Estado do Piaui. Duzentos e sessenta e cinco individuos foram investigados pelos seguintes metodos: pesquisa direta, hemocultura, imunofluorescencia indireta (IFA), teste imunoabsorvente ligado a enzima (ELISA), e ELISA de competicao (C-ELISA) com a utilizacao do anticorpo monoclonal TCF87 contra um antigeno do T.cruzi com 25kd. A IFA foi positiva em 14,3 por cento dos individuos, ELISA em 14,7 por cento e C-ELISA em 13,2 por cento. Este ultimo teste mostrou-se aplicavel no diagnostico sorologico da Doenca de Chagas.
Subject(s)
Infant, Newborn , Child, Preschool , Child , Adult , Infant , Adolescent , Middle Aged , Humans , Animals , Chagas Disease/epidemiology , Brazil/epidemiology , Chagas Disease/diagnosis , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique , PrevalenceABSTRACT
A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hemoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
