ABSTRACT
BACKGROUND: Glycoprotein B (gB) has been implicated in determining the pathogenicity and clinical outcomes of human cytomegalovirus (HCMV) disease. OBJECTIVE: The purpose of this study was to assess the prevalence of gB genotypes in allogeneic hematopoietic stem cell transplantation (allo-HSCT) and the relationship between it and cytokine levels in saliva and blood samples. The impact of these parameters on patients' survival was also investigated. METHODS: Samples were obtained from 63 patients receiving an allo-HSCT. HCMV gB genotyping was carried out by multiplex nested PCR. The cytokine levels were assessed using ELISA assay. RESULTS: A single or mixed genotype infection was detected in the saliva and blood of 36/63 and 52/63 subjects, respectively. Patients with gB2 in their saliva showed lower IL-10 levels in comparison with patients without gB2. Reduced blood levels of IFN-γ and IL-1ß were also found in recipients with the HCMV gB4 genotype compared with patients without it. Decreased IL-1ß and increased IL-10 blood levels were associated with lower survival. However, HCMV gB genotypes have no impact on patient outcome. CONCLUSION: Decreased IL-1ß and increased IL-10 levels in the blood are associated with lower survival. HCMV genotypes are associated with different cytokine levels in saliva and blood.
Subject(s)
Cytokines/analysis , Cytomegalovirus Infections/immunology , Cytomegalovirus/genetics , Hematopoietic Stem Cell Transplantation , Viral Envelope Proteins/genetics , Adolescent , Adult , Child , Child, Preschool , Cytokines/blood , Cytomegalovirus/immunology , Female , Follow-Up Studies , Genotype , Humans , Immunocompromised Host , Interferon-gamma/analysis , Interferon-gamma/blood , Interleukin-10/analysis , Interleukin-10/blood , Interleukin-1beta/analysis , Interleukin-1beta/blood , Interleukin-6/analysis , Interleukin-6/blood , Male , Middle Aged , Opportunistic Infections/virology , Saliva/chemistry , Saliva/immunology , Survival Rate , Transplantation Conditioning , Transplantation, Homologous , Tumor Necrosis Factor-alpha/analysis , Viral Envelope Proteins/immunology , Young AdultABSTRACT
OBJECTIVE: The purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (HCMV) in allogeneic hematopoietic stem cell transplant patients by real time PCR compared with blood. MATERIALS AND METHODS: Saliva and blood samples were sampled weekly in 30 allogeneic hematopoietic stem cell transplant patients until 100 days after transplant. Total genomic DNA, extracted from saliva and whole-blood samples, was used for HCMV real time PCR. Nonparametric tests were performed, and P value Subject(s)
Cytomegalovirus/isolation & purification
, DNA, Viral/analysis
, Hematopoietic Stem Cell Transplantation
, Saliva/virology
, Adolescent
, Adult
, Antigens, Viral/analysis
, Antigens, Viral/blood
, Antiviral Agents/therapeutic use
, Child
, Cytomegalovirus/genetics
, Cytomegalovirus/immunology
, Cytomegalovirus Infections/diagnosis
, Cytomegalovirus Infections/drug therapy
, DNA, Viral/blood
, Feasibility Studies
, Female
, Follow-Up Studies
, Ganciclovir/therapeutic use
, Humans
, Longitudinal Studies
, Male
, Middle Aged
, Phosphoproteins/analysis
, Phosphoproteins/blood
, Polymerase Chain Reaction
, Prospective Studies
, Transplantation, Homologous
, Viral Load
, Viral Matrix Proteins/analysis
, Viral Matrix Proteins/blood
, Viremia/virology
, Virus Activation
, Young Adult
ABSTRACT
BACKGROUND: Recurrent aphthous stomatitis (RAS) is characterized by recurrent episodes of oral ulceration in an otherwise healthy individual. Some reports in the literature indicate that RAS may have immunological, psychological, genetic and microbiological bases. The purpose of the present study was to investigate the possible association between interleukin-1beta (IL-1beta) +3954 (C/T) genetic polymorphism and RAS in a sample of Brazilian patients. SUBJECTS AND METHODS: Sixty-two consecutive subjects affected by minor and major forms of RAS and 62 healthy volunteers were genotyped at IL-1beta (+3954). The chi-squared test was used for statistical analysis. RESULTS: A significant increase in the high production of IL-1beta genotype CT was observed in the group with RAS (P = 0.01). After stratifying RAS patients according to the mean number of lesions per episode, a significant difference was only observed between patients with >or=3 lesions in each episode and control. CONCLUSION: There is an increased frequency of polymorphism associated with high IL-1beta production in RAS patients.
