Subject(s)
Pneumothorax/diagnosis , Alcoholism , Diagnosis, Differential , Forensic Pathology , Head Injuries, Closed/diagnosis , Humans , Hyperemia/pathology , Male , Mediastinal Emphysema/pathology , Middle Aged , Pneumopericardium/pathology , Postmortem Changes , Posture , Rib Fractures/pathology , Subcutaneous Emphysema/pathologyABSTRACT
The paper describes a fatal autoerotic accident of a 23-year-old man who derived sexual pleasure from painful irritation of the peritoneum by sharp force. For this purpose he clamped two knife blades into a vice suspended from a rope-pulley construction so that he could lower it to pierce the abdominal skin with the tips of the knives. When one of the ropes broke, the vice with the knives fell onto the man's belly piercing the inferior vena cava and leading to death by exsanguination. This, to the authors' knowledge, has been the first ever report of a fatal autoerotic accident in which sexual pleasure was to be obtained from painful irritation of the peritoneum.
Subject(s)
Accidents/legislation & jurisprudence , Masturbation/pathology , Pain/pathology , Peritoneum/injuries , Self Mutilation/pathology , Wounds, Stab/pathology , Adult , Cause of Death , Humans , Male , Peritoneum/pathologyABSTRACT
The genetic polymorphism of human C6 was investigated in Germany using an improved technique: polyacrylamide gel isoelectric focusing and subsequent direct immunofixation with monospecific C6 antisera. Typing of C6 was performed on native serum samples from 1,775 unrelated persons. The gene frequencies in the population study were as follows: C6*A 0.6313, C6*B 0.3566 and the rare alleles C6*R 0. 0121. In total, 8 rare allotypes were analysed. The gene frequencies obtained are in good agreement with those previously published.
Subject(s)
Complement C6/genetics , Polymorphism, Genetic , Alleles , Complement C6/isolation & purification , Female , Gene Frequency , Germany , Humans , Immunophenotyping , Isoelectric Focusing , MaleABSTRACT
The genetically inherited polymorphic plasma protein types have always been considered stable for lifetime in humans. Most of these proteins are synthetised in the liver. Phenotypes for 14 plasma proteins in donors and recipients of liver transplants prior to and after transplantation were determined in 15 patients who had undergone liver transplantation at the university hospitals Charité and Rudolf Virchow in Berlin. The plasma proteins investigated were HP, TF, GC, PI, ORM1, ITI, A2HS, PLG, FXIIIB, BF, C3, C6, C8 and FH. Evidence was provided of irreversible change from the recipient type to the donor type in at least one patient for all the systems investigated. This is the first time such data have been obtained for ITI, A2HS, C8 and FH. These results clearly support the point that the dogma of life-long stability of genetically determined protein phenotypes is merely of limited validity. Against the background of good long-term results of liver transplantation, there are consequences for the practice of legal medicine in the particular context of certification of parentage, identification and stain analysis.
Subject(s)
Blood Proteins/genetics , Liver Transplantation , Paternity , Phenotype , Polymorphism, Genetic , Blood Stains , Humans , Isoelectric Focusing , Time Factors , Tissue DonorsABSTRACT
Within the C6/C7/C9 Session of the VIIth Complement Genetics Workshop and Conference, various C6 protein allotypes collected from four laboratories were examined using polyacrylamide gel isoelectric focusing (PAGIF) followed by direct immunofixation. Nineteen different variants including 17 recognized allotypes and introducing two new allotypes were distinguished besides the two common allotypes C6 A and C6 B. They were designated basically according to the previous statement on C6 nomenclature. Details on C6 DNA markers and associations of C6 markers with deficiencies of terminal complement components are published in the preceding paper of this issue.
Subject(s)
Complement C6/classification , Complement C6/genetics , Complement C6/standards , Genetic Variation , Humans , Isoelectric Focusing , Reference StandardsABSTRACT
The inter-alpha-trypsin inhibitor (ITI) polymorphism was analysed in a German population, using polyacrylamide gel isoelectric focusing with subsequent direct immunofixation with monospecific ITI antisera. Gene frequencies of ITI*1, ITI*2, ITI*3, and ITI*4 were calculated to be 0.6150, 0.3753, 0.0078 and 0.0019, respectively. In our study the allele ITI*4 is described for the first time in a German population.
Subject(s)
Alpha-Globulins/genetics , Isoelectric Focusing/methods , Polymorphism, Genetic , Alleles , Gene Frequency , Germany , Humans , Immune Sera , PhenotypeABSTRACT
When neuraminidase-treated sera are analyzed by agarose gel isoelectric focusing, the factor B (BF) banding pattern is reduced to predominantly one major band without cathodically positioned bands. This not only makes unequivocal typing of BF allotypes possible but also the reliable distinction of all BF F subtype phenotypes with delimitation of "BF F subtype variants". With this new method, serum aging affects the BF determination to a lesser extent than when applying methods that separate native sera. We show that sialylation is not responsible for the BF F subtype polymorphism. All of the investigated BF allotype bands, including those characteristic of the subtypes, show functional hemolytic activity. The banding pattern after removal of neuraminic acid residues ranges from pH 6.8 to 7.3 for factor B, from pH 5.3 to 5.9 for the Ba fragment, and from pH 8.2 to 8.7 for the Bb fragment. The protein structure of factor B is also discussed. Eliminating the superimposition of bands in different BF allotypes, as demonstrated by these methods, proved to be necessary for the detection of hypomorphic BF gene products (BF QL), which are expressed by assumed BF*Q0 alleles in heterozygous genotypes. This allows investigation of BF*Q0 alleles on a protein level, which complements molecular genetic approaches.
Subject(s)
Complement Factor B/genetics , Neuraminidase , Polymorphism, Genetic/genetics , Blood Preservation , Humans , Hydrogen-Ion Concentration , Immunoblotting , Isoelectric Focusing , PhenotypeABSTRACT
GC-subtyping was carried out on blood stains, that had been made on glass and stored under room temperature-conditions. Using isoelectric focusing in polyacrylamide gel, followed by transfer to nitrocellulose membrane by semi-dry-electroblotting and detection with enzyme-linked antibody complex the GC-detection was possible even after 64 days. Methodical problems are discussed.
Subject(s)
Blood Grouping and Crossmatching/methods , Immunoblotting , Isoelectric Focusing , Vitamin D-Binding Protein/analysis , Humans , Phenotype , Vitamin D-Binding Protein/geneticsSubject(s)
Chromosome Aberrations/genetics , Genes, Dominant/genetics , Lung Diseases, Obstructive/genetics , Phenotype , alpha 1-Antitrypsin Deficiency , Chromosome Disorders , Humans , Lung Diseases, Obstructive/diagnosis , Pulmonary Emphysema/genetics , Risk Factors , alpha 1-Antitrypsin/geneticsABSTRACT
DNA fingerprints were established from 3 months old hemolysed blood samples. In spite of partial degradation of the DNA, the hybridization with the oligonucleotide probe (GTG)5 allowed unequivocal identification of the source of the blood.
Subject(s)
Blood Grouping and Crossmatching/methods , Blood Preservation , DNA Probes , Hemolysis , Accidents, Traffic/legislation & jurisprudence , Ethanol/pharmacokinetics , Humans , TemperatureABSTRACT
For the last 25 years, antisera for testing P1 in man have been obtained by i.v. injections in goats using hydatid cyst fluid from livers of pigs infected by Echinococcus granulosus. Due to the fact that this antigen has become very rare today, efforts have been made by the authors to substitute it by different antigens and to save antigen by modifying the technique of immunisation as well. By this, very high titres up to 1:4096 could be reached after specification absorption.