ABSTRACT
INTRODUCTION: In recent years, several non-invasive imaging methods have been introduced to facilitate studies in dermatology and cosmetic research, almost completely replacing invasive methods such as biopsy. Imaging devices have proven to be useful tools in skin analysis and therapy monitoring. This review aimed to investigate the most recent studies in cosmetic dermatology the imaging technology and methods that are being used to assess skin characteristics and summarize its fundamentals, possible applications, advantages, and limitations, and to give a future perspective to the clinical trials. EVIDENCE ACQUISITION: For that, a literature review was carried out in the main scientific database platforms and the studies associating skin image analysis with dermatology and cosmetic research were selected and discussed. EVIDENCE SYNTHESIS: It was possible to infer that skin image analyses are not only practical and effective, but have also become increasingly essential for the skin sciences. The in vivo and real-time image analyses allow a more complete evaluation and the follow-up of the same region for different periods. It was also possible to observe that macroscopic, microscopic, and mesoscopic imaging methods are complementary, allowing different approaches in the same study. CONCLUSIONS: These technologies are expected to evolve more and more quickly in the near future.
ABSTRACT
Even being a bacterial purine nucleoside phosphorylase (PNP), which normally shows hexameric folding, the Mycobacterium tuberculosis PNP (MtPNP) resembles the mammalian trimeric structure. The crystal structure of the MtPNP apoenzyme was solved at 1.9 A resolution. The present work describes the first structure of MtPNP in complex with phosphate. In order to develop new insights into the rational drug design, conformational changes were profoundly analyzed and discussed. Comparisons over the binding sites were specially studied to improve the discussion about the selectivity of potential new drugs.
