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1.
Turk J Pharm Sci ; 20(3): 141-148, 2023 Jul 07.
Article in English | MEDLINE | ID: mdl-37417007

ABSTRACT

Objectives: Trachystemon orientalis (L.) G. Don, colloquially known in Türkiye as "kaldirik", is an edible plant belonging to the Boraginaceae. This plant has been practiced in traditional medicine for many years for its various therapeutic benefits. The effectiveness and chemical composition of plants can vary depending on their parts, age, and extraction solvent. Therefore, the current study aimed to define the biological activities of various parts and extracts of T. orientalis, which were collected in distinct seasons as young and mature, and investigate the main component responsible for these biological effects. Material and Methods: Plant material was collected in different seasons from the northwest of Türkiye. 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities were investigated to assess antiradical and antioxidant potential of the extracts. Anti-inflammatory activity of the extracts was also tested using human red blood cell membrane stabilizing method. Folin-Ciocalteu test was conducted to determine the total phenolic content. Reverse phase-high performance liquid chromatography-photodiode array detector (RP-HPLC-PDA) analysis was performed. Results: Both methanol and aqueous extracts exhibited significant radical scavenging and anti-inflammatory activities compared with control (p<0.05). The highest percentage of inhibition on ABTS and DPPH free radicals was obtained in aqueous extracts of the mature herbs and roots, respectively. Methanol extracts of the mature roots and herbs exhibited the strongest anti-inflammatory capacity. Rosmarinic acid possessed a much higher antioxidant and anti-inflammatory effect than the reference compounds used in each assay in our study. High rosmarinic acid content of the extracts suggests that the compound responsible for the great biological activity potential is rosmarinic acid. Conclusion: To the best of our knowledge, the presence of rosmarinic acid in herbs and roots of T. orientalis was shown for the first time in our present study. Phytochemical composition and effective biological activities of T. orientalis explain its traditional use and indicate its significant potential in pharmaceutical industry applications.

2.
Molecules ; 25(7)2020 Mar 30.
Article in English | MEDLINE | ID: mdl-32235622

ABSTRACT

Lactuca L. species belong to the Asteraceae family and these plants are traditionally used for therapeutic purposes around the world. The dried milky latex of L. serriola is known as "lettuce oil" and is used as a sedative in Turkey. This study aimed to evaluate the sedative effects and analyze the chemical compositions of latexes obtained from some Lactuca species growing in Turkey. The sedative effects were evaluated through various behavioral tests on mice. For this purpose, latexes were obtained from L. glareosa Boiss., L. viminea (L.) J. Presl and C. P, L. mulgedioides (Vis and Pancic) Boiss. and Kotschy ex. Boiss., L. saligna L., and L. serriola L. The latex from L. saligna showed the highest sedative effects, whilst L. serriola and L. viminea latexes also displayed significant sedative effects compared to the control group at a dose of 100 mg/kg. However, the latexes from L. glareosa and L. mulqedioides did not exhibit any sedative effects on mice. Characteristic sesquiterpene lactones (lactucin, lactucopicrin, 11,13ß-dihydrolactucin, and 11,13ß-dihydrolactucopicrin) were determined qualitatively and quantitatively by high-performance liquid chromatography (HPLC). Lactucin was identified as the main component.


Subject(s)
Hypnotics and Sedatives , Lactuca/chemistry , Latex/chemistry , Animals , Hypnotics and Sedatives/chemistry , Hypnotics and Sedatives/isolation & purification , Hypnotics and Sedatives/pharmacology , Lactuca/growth & development , Male , Mice , Mice, Inbred BALB C , Turkey
3.
Turk J Pharm Sci ; 15(2): 125-129, 2018 Aug.
Article in English | MEDLINE | ID: mdl-32454650

ABSTRACT

OBJECTIVES: In this study, the antioxidant capacity, antimicrobial activity and phenolic contents of aerial parts and roots extracts of three endemic Ononis L. (Leguminosae) species (O. sessilifolia Bornm., O. basiadnata Hub. & Mor., O. macrosperma Hub. & Mor.) were investigated for the first time. MATERIALS AND METHODS: The phenolic contents of the extracts [water and ethanol (EtOH)] and fractions [dichloromethane, EtOAc and n-butanol] were determined using Folin-Ciocalteu's phenol reagent. Also, their antioxidant capacities were studied using qualitative DPPH˙ (1,1-diphenyl-2-picrylhdrazyl radical) and TBA assays. The antimicrobial activity of these extracts and fractions compared with standard antibiotics were studied using disc diffusion assays against various Gram-positive and Gram-negative bacteria and fungi. RESULTS: The total phenolic contents of the water extracts were found to range between 14.78-80.33 mg/g, and the EtOH extracts ranged from 67.19-145.33 mg/g. EtOAc fractions of the three species were rich in terms of total phenolic contents when compared with other extracts (242.56-620.89 mg/g). The most significant results in the TBA assays were obtained in EtOH extracts of O. macrosperma (IC50=0.13±0.17 µg/mL), O. sessilifolia (IC50=1.41±0.58 µg/mL) and root (IC50=1.96±0.39 µg/mL). CONCLUSION: EtOAc fractions rich in phenolic content were also found to be the most effective in antioxidant activity assays. Although all water extracts had no antimicrobial activity, EtOH extracts and n-butanol fractions showed generally moderate activity against bacteria. Some EtOAc fractions except for O. sessilifolia showed less activity against Escherichia coli, Staphylococcus aureus, MRSA and Candida albicans.

4.
Turk J Pharm Sci ; 14(2): 134-140, 2017 Aug.
Article in English | MEDLINE | ID: mdl-32454604

ABSTRACT

OBJECTIVES: The leaf and stem anatomical characteristics of three endemic Ononis L. (Leguminosae) species were studied for the first time for the evaluated in terms of systematic of similarities and differences in these taxa. MATERIALS AND METHODS: Ononis sessilifolia Bornm., Ononis basiadnata Hub.-Mor. and Ononis macrosperma Hub.-Mor. were collected from different provinces of Turkey. In this anatomical study, transversal and superficial sections from the leaves and transversal sections from stems of the species were taken and examined by appropriate reagends. Illustrations were obtained by using an Olympus U-DA 2K 17149 drawing tube attached to an Olympus BX50 microscope. The microphotographs were taken by a Leica DM 4000 B microscope. RESULTS: O. macrosperma is bifacial leaf, the others are monofacial leaf. The leaf of O. basiadnata has eglandular trihomes. If the anatomical similarities between species are to be listed, Rubiaceae type stoma was observed in all three species and solitary crystals were found in stem and leaf sections. CONCLUSION: Anatomical characteristics, such as mesophyll structure, transmission bundles and crystal structures, may contribute to the taxonomy of Ononis species for future work.

5.
Nat Prod Commun ; 11(3): 397-400, 2016 Mar.
Article in English | MEDLINE | ID: mdl-27169190

ABSTRACT

In this study, colchicine (CLN) and colchicoside (CLS) contents of the methanolic extracts of the seeds of Colchicum speciosum Steven that were collected from Uzungöl, Trabzon and also the seeds belonging to two different samples of Gloriosa superba Linn. imported from India were compared by using RP-LC (Reversed Phase High Pressure Liquid Chromatography). This proposed method is advantageous in terms of sample preparation and selective separation of the compounds. Also the method was successfully validated in accordance with the International Conference on Harmonization (ICH) guideline acceptance criteria for system suitability, linearity and range, precision, specificity and accuracy. As a conclusion of this analysis, the colchicoside and colchicine contents of G. superba (GSI), G. superba (GSII) and C. speciosum (CS) were found to be 312.9 mg/100 g and 333.1 mg/100 g; 434.0 mg/100 g and 471.1 mg/100 g, and 51.9 mg/100 g and 75.9 mg/100 g, respectively.


Subject(s)
Chromatography, Liquid/methods , Colchicine/analogs & derivatives , Colchicine/chemistry , Liliaceae/chemistry , Seeds/chemistry , Reproducibility of Results , Sensitivity and Specificity
6.
Nat Prod Commun ; 11(11): 1665-1666, 2016 Nov.
Article in English | MEDLINE | ID: mdl-30475502

ABSTRACT

The genus Epimedium is rich in terms of flavonoids, of which icariin, epimedin A, epimedin B and epimedin C are known especially to be biologically active. Therefore, it is important to quantify these compounds. In this study, a HPLC method coupled with DAD detection was developed and validated for the determination of icariin, epimedin A, epimedin B and epimedin C in Epimedium species growing in Turkey. The chromatographic separation was performed using a gradient system with a mobile phase of 0.1% formic acid (A) and acetonitrile (B) applied at a flow rate of 1 mL/min using.a diode array detector. The highest values were, respectively, icariin 0.65%, epimedin A 0.13%, epimedin B 0.11%, epimedin C 0.06%. The highest values were obtained from the materials collected in Uzungol (Trabzon-Turkey).


Subject(s)
Epimedium/chemistry , Flavonoids/chemistry , Chromatography, High Pressure Liquid/methods , Turkey
7.
J Pharm Biomed Anal ; 72: 155-8, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23146241

ABSTRACT

A new sildenafil analogue was found to be added illegally to a energy drink marketed for the enhancement of sexual function. The structure was determined as 1-[4-propoxy-3-(6,7-dihydro-1-methyl-7-oxo-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-5-yl)phenylsulfonyl]-4-methylpiperazine. Owing to the inclusion of one more methyl group to sildenafil (on C-21), the detected compound was called "propoxyphenyl sildenafil". The sample was purified with column chromatography. The UV, IR, LC/MS (ESI) and completely assigned NMR data of propoxyphenyl sildenafil is reported. Having compared the structure with sildenafil, the results showed that the 2-ethoxy group (at the position on C-19) has been replaced by propoxy group.


Subject(s)
Energy Drinks/analysis , Piperazines/analysis , Sulfones/analysis , Chromatography, Liquid/methods , Magnetic Resonance Spectroscopy/methods , Mass Spectrometry/methods , Purines/analysis , Sildenafil Citrate , Spectrophotometry, Infrared/methods , Spectrophotometry, Ultraviolet/methods
8.
J Ethnopharmacol ; 135(1): 63-70, 2011 Apr 26.
Article in English | MEDLINE | ID: mdl-21354296

ABSTRACT

AIM OF THE STUDY: Ononis L. species have been used for healing of wounds, eczema and rheumatic complaints, against skin cancer and lesions and topically used as antiseptic and antimicrobial agent in folk medicine. In the present study, the aqueous and methanolic extracts of three endemic Ononis species growing in Turkey, including Ononis sessilifolia Bornm., Ononis basiadnata Hub.-Mor. and Ononis macrosperma Hub.-Mor. were assessed for their in vivo wound healing and anti-inflammatory activities. MATERIALS AND METHODS: In vivo wound healing activities of the plants were evaluated by using linear incision and circular excision experimental models subsequently histopathological analysis. The healing potential was comparatively assessed with a reference ointment Madecassol(®), which contains 1% extract of Centella asiatica. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. RESULTS: The aqueous and ethanolic extracts of the aerial parts of O. macrosperma demonstrated the highest activity in both wound models and significant anti-inflammatory activity in acetic acid-induced increase in capillary permeability test. Furthermore, ethanolic extract of the aerial parts of O. sessilifolia showed anti-inflammatory effect but was devoid of wound healing activity. The rest of the species did not show remarkable wound healing effect. Ethyl acetate fraction of O. macrosperma ethanolic extract demonstrated significant but reduced wound healing activity, which revealed a possible combined effect. The results of histopathological examination supported the outcome of linear incision and circular excision wound models. CONCLUSION: The experimental study revealed that the aerial parts of O. macrosperma display remarkable wound healing and anti-inflammatory activities.


Subject(s)
Anti-Inflammatory Agents/therapeutic use , Fabaceae , Phytotherapy , Plant Extracts/therapeutic use , Skin/drug effects , Wound Healing/drug effects , Wounds, Stab/drug therapy , Animals , Anti-Infective Agents/pharmacology , Anti-Inflammatory Agents/pharmacology , Capillaries/drug effects , Centella , Disease Models, Animal , Male , Mice , Plant Components, Aerial , Plant Extracts/pharmacology , Rats , Rats, Sprague-Dawley , Skin/injuries , Triterpenes/pharmacology , Turkey
9.
Nat Prod Commun ; 5(12): 1951-4, 2010 Dec.
Article in English | MEDLINE | ID: mdl-21299129

ABSTRACT

The essential oils from aerial parts of Ferulago pachyloba (Fenzl) Boiss., F. platycarpa Boiss. & Bal., F. isaurica Penmen, and F. longistylis Boiss. (Apiaceae) were obtained by hydrodistillation and analyzed by GC and GC-MS. The highest oil yield (1.50%) was obtained from F. pachyloba followed by F. longistylis (0.16%), F. isaurica (0.08%) and F. platycarpa (0.07%). Fifty-three compounds were identified in the oil of F. pachyloba with (Z)-beta-ocimene (25.7%) and alpha-pinene (9.8%) as main constituents; sixty-seven in the oil of F. platycarpa with 2,3,6-trimethylbenzaldehyde (29.8%) and cis-chrysanthenyl acetate (24.2%) as main components; seventy-eight in the oil of F. isaurica with nonacosane (25.5%) and hexadecanoic acid (14.8%) as main constituents; and fifty-nine in the oil of F. longistylis with 2,3,6-trimethylbenzaldehyde (32.7%) and bornyl acetate (12.6%) as main components. Quantitative and qualitative differences in the oil compositions of these four species were observed.


Subject(s)
Apiaceae/chemistry , Oils, Volatile/analysis , Chromatography, Gas , Gas Chromatography-Mass Spectrometry
10.
Phytochemistry ; 69(3): 729-37, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18022204

ABSTRACT

Seven steroidal glycosides, along with one known glycoside, were isolated from the rhizomes of Ruscus hypophyllum (Liliaceae). Comprehensive spectroscopic analysis, including 2D NMR spectroscopy, and the results of acid hydrolysis allowed the chemical structures of the compounds to be assigned as (23S,25R)-23-hydroxyspirost-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (1), 1beta-hydroxyspirosta-5,25(27)-dien-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (2), (22S)-16beta,22-dihydroxycholest-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (3), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-22-hydroxycholest-5-en-3beta-yl O-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (4), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-22-hydroxycholest-5-en-3beta-yl beta-d-glucopyranoside (5), (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-3beta,22-dihydroxycholest-5-en-1beta-yl O-alpha-l-rhamnopyranosyl-(1-->2)-(3,4-di-O-acetyl-beta-d-xylopyranoside) (6), and (22S)-16beta-[(beta-d-glucopyranosyl)oxy]-3beta,22-dihydroxycholest-5-en-1beta-yl O-alpha-l-rhamnopyranosyl-(1-->2)-O-[beta-d-xylopyranosyl-(1-->3)]-beta-d-xylopyranoside (7), respectively. This is the first isolation of a series of cholestane glycosides from a Ruscus species.


Subject(s)
Glycosides/chemistry , Liliaceae/chemistry , Rhizome/chemistry , Steroids/chemistry , Glycosides/isolation & purification , Magnetic Resonance Spectroscopy/methods , Magnetic Resonance Spectroscopy/standards , Molecular Structure , Reference Standards , Species Specificity , Steroids/isolation & purification
11.
Phytochem Anal ; 17(1): 20-4, 2006.
Article in English | MEDLINE | ID: mdl-16454472

ABSTRACT

(2S, 3S)-Taxifolin 3-O-arabinoside, a new dihydroflavonol glycoside, together with the known substances, (2R 3R)-taxifolin 3-O-arabinoside, astragalin, isoquercitrin, and cosmosiin, were isolated from the leaves of Trachelospermum jasminoides var. pubescens. Structural elucidation was carried out by spectroscopic methods, and the absolute configurations of C-2 and C-3 in taxifolin 3-O-arabinosides were determined on the basis of circular dichroism. Unlike the dihydroflavonol glycosides, (2R 3R)-taxifolin 3-O-glucoside and (2R, 3R-taxifolin 3-O-arabinoside, the novel (2S, 3S)-taxifolin 3-O-arabinoside is not effective as a zoospore attractant of the plant pathogenic fungus Aphanomyces cochlioides.


Subject(s)
Apocynaceae/chemistry , Glycosides/chemistry , Chromatography, High Pressure Liquid , Circular Dichroism , Molecular Structure , Plant Leaves/chemistry , Spectrometry, Mass, Fast Atom Bombardment , Spectrophotometry, Ultraviolet
13.
Biol Pharm Bull ; 27(7): 1140-3, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15256757

ABSTRACT

The inhibitory effects of 40 extracts prepared from 38 traditional Turkish folk medicines on human aldose reductase (h-AR) and hematological activity were investigated. Seven plants containing 5 species of Cistus genus exhibited a potent inhibition of h-AR. Ferulago amani (root) inhibited the platelet aggregation induced by sodium arachidonate, while C. laurifolius (fruit) was found to possess strong inhibition in the blood coagulation assay. An AcOEt extract derived from the leaf of C. laurifolius was purified to isolate three known flavonoids. The activity of one, quercetin-3-O-methyl ether, was found to be as potent as that of eparlestat, which is known to be a remedy for treating complications associated with diabetes.


Subject(s)
Aldehyde Reductase/antagonists & inhibitors , Anticoagulants/pharmacology , Cistus , Medicine, Traditional , Platelet Aggregation Inhibitors/pharmacology , Quercetin/analogs & derivatives , Quercetin/pharmacology , Aldehyde Reductase/metabolism , Anticoagulants/isolation & purification , Blood Coagulation/drug effects , Blood Coagulation/physiology , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/pharmacology , Humans , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Plant Structures , Platelet Aggregation/drug effects , Platelet Aggregation/physiology , Platelet Aggregation Inhibitors/isolation & purification , Quercetin/isolation & purification , Turkey
14.
Biol Pharm Bull ; 27(5): 702-5, 2004 May.
Article in English | MEDLINE | ID: mdl-15133249

ABSTRACT

Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducing powers and the amount of total phenolic compounds of aqueous and/or methanolic extracts of some medicinal plants used in Eastern Turkey were studied. These plants are Prangos ferulacea (CASIR), Sedum sempervivoides (HOROZ LELESI), Malva neglecta (EBEMGUMECI), Cruciata taurica (SARILIK OTU), Rosa pimpinellifolia (KOYUN GOZU), Galium verum subsp. verum (MADAVUR OTU), Urtica dioica (ISIRGAN). The highest peroxidation inhibitions were shown by aqueous extracts of C. taurica and R. pimpinellifolia (IC(50): 0.00022 mg/l and IC(50): 23 mg/l, respectively). However, the highest DPPH radical scavenging activity, reducing power and the amount of phenolic compounds were shown by R. pimpinellifolia. The lowest antioxidant properties were shown by aqueous extract of M. neglecta.


Subject(s)
Antioxidants/pharmacology , Plant Extracts/pharmacology , Plants, Medicinal , Antioxidants/isolation & purification , Apiaceae , Crassulaceae , Free Radical Scavengers/pharmacology , Lipid Peroxidation/drug effects , Lipid Peroxidation/physiology , Malvaceae , Plant Extracts/isolation & purification , Rosaceae , Rubiaceae , Urtica dioica
15.
Phytochem Anal ; 14(1): 48-53, 2003.
Article in English | MEDLINE | ID: mdl-12597255

ABSTRACT

The threo and erythro forms of guaiacylglycerol-7'-O-methyl 8'-vanillic acid ethers, threo and erythro guaiacylglycerol 8'-vanillin ethers, and threo guaiacylglycerol 8'-(4-hydroxymethyl-2-methoxyphenyl) ether have been isolated from fruits of Boreava orientalis. Structural determinations were made on the basis of UV, MS, 1H- and 13C-NMR spectral data, including two-dimensional shift correlation. The relative configurations were assigned on the basis of 1H-NMR chemical shifts.


Subject(s)
Brassicaceae/chemistry , Guaifenesin/analogs & derivatives , Guaifenesin/analysis , Guaifenesin/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure
16.
Chem Pharm Bull (Tokyo) ; 50(2): 185-92, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11848207

ABSTRACT

Investigation of the constituents of the fruits of Morus alba LINNE (Moraceae) afforded five new nortropane alkaloids (1-5) along with nor-psi-tropine (6) and six new amino acids, morusimic acids A-F (7-12). The structures of the new compounds were determined to be 2alpha,3beta-dihydroxynortropane (1), 2beta,3beta-dihydroxynortropane (2), 2alpha,3beta,6exo-trihydroxynortropane (3), 2alpha,3beta,4alpha-rihydroxynortropane (4), 3beta,6exo-dihydroxynortropane (5), (3R)-3-hydroxy-12-[(1S,4S)-4-[(1S)-1-hydroxyethyl]-pyrrolidin-1-yll-dodecanoic acid-3-O-beta-D-glucopyranoside (7), (3R)-3-hydroxy-12-[(1S,4S)-4-[(1S)-1-hydroxyethyl]-pyrrolidin-1-yll-dodecanoic acid (8), (3R)-3-hydroxy-12-1(1R,4R,5S)-4-hydroxy-5-methyl-piperidin-1-yll-dodecanoic acid-3-O-beta-D-glucopyranoside (9), (3R)-3-hydroxy-12-[(1R,4R,5S)-4-hydroxy-5-methyl-piperidin-1-yll-dodecanoic acid (10), (3R)-3-hydroxy-12-[(1R,4R,5S)-4-hydroxy-5-hydroxymethyl-piperidin-1-yl]-dodecanoic acid-3-O-beta-D-glucopyranoside (11), and (3R)-3-hydroxy-12-[(1R,4S,5S)-4-hydroxy-5-methyl-piperidin-1-yl]-dodecanoic acid (12) on the basis of spectral and chemical data.


Subject(s)
Alkaloids/isolation & purification , Amino Acids/isolation & purification , Moraceae/chemistry , Alkaloids/chemistry , Amino Acids/chemistry , Fruit , Magnetic Resonance Spectroscopy , Turkey
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