Synovial fluid NMR-based metabolomics in septic and aseptic revision total knee arthroplasty: Implications on diagnosis and treatment.

Periprosthetic joint infection (PJI) is one of the most challenging complications following total knee arthroplasty. Despite its importance, there is a paucity of reports in the literature regarding its pathogenesis. Recently, cellular metabolic reprogramming has been shown to play an important role in the progression and outcome of infectious diseases. Therefore, the aim of this study was to evaluate the metabolites composition of the synovial fluid from patients with PJI or aseptic failure of total knee arthroplasties. The synovial fluids from 21 patients scheduled for revision total knee arthroplasty (11 with the diagnosis of PJI and 10 with aseptic failures) were analyzed using 1D 1H NMR spectroscopy. Univariate and multivariate statistical analyzes were used to identify metabolites that were differentially abundant between those groups. A total of 28 metabolites were identified and five of them found to be differentially abundant between infected and non-infected synovial fluids. Lactate, acetate and 3-hydroxybutyrate were found to be in a higher concentration, and glucose and creatine were found reduced in the synovial fluid from PJI patients. Synovial fluid from patients with PJI exhibit a distinct metabolic profile, possibly reflecting metabolic adaptation that occurs in the infected periprosthetic microenvironment. Further research and studies are warranted to gain a broader insight into the metabolic pathways engaged by both pathogen and immune cells in the context of a PJI.

Audiovisual indígena Xavante na luta por direitos / Indigenous Xavante video in the fight for rights

Este artigo busca evidenciar o uso do vídeo indígena Xavante como arma na luta por direitos, focalizando o processo de montagem e exibição do documentário Wai'á Rini (2017), do cineasta indígena Divino Tserewahú. Baseia-se em pesquisa realizada em acervos audiovisuais e em entrevistas junto aos indígenas Xavante da comunidade de Sangradouro e Pimentel Barbosa em Mato Grosso. As entrevistas com realizadores Xavante e oficina foram realizadas entre 2014 e 2018, sendo as primeiras abertas e não estruturadas, a última foi delineada a partir de critérios técnicos orientados à realização audiovisual. Argumenta-se que a montagem concretiza um vasto campo de possibilidades de lutas por direitos indígenas, o mesmo podendo ser estendido à exibição que também pode ser convertida em um instrumento de luta, como ocorre com o vídeo Wai'á Rini (2017). Narra-se, ainda, parceria com realizadores indígenas de Sangradouro para formação de jovens gerações de cineastas Xavante, com ênfase na atuação do realizador Divino Tserewahú.(AU)

This article seeks to demonstrate the use of the Xavante indigenous video as a weapon in the fight for rights, focusing on the process of assembling and showing the documentary Wai'á Rini (2017), by the indigenous filmmaker Divino Tserewahú. It is based on research carried out in audiovisual collections and in identification with the Xavante Indians of the community of Sangradouro and Pimentel Barbosa in Mato Grosso. It is argued that the editing materializes a vast field of possibilities for struggles for indigenous rights, the same being extended to the exhibition that can also be converted into a fighting instrument, as is the case with the video Wai'á Rini (2017). It also narrates a partnership with indigenous filmmakers from Sangradouro to train young generations of Xavante filmmakers.(AU)

Photocurrent Response and Progesterone Degradation by Employing WO3 Films Modified with Platinum and Silver Nanoparticles.

The effect of silver (Ag0 ) and platinum (Pt0 ) metallic nanoparticles (NPs) on WO3 film was investigated by studying the photocurrent response under polychromatic irradiation. The structural phase revealed by X-ray diffraction analysis indicates a monoclinic crystal nanostructure. WO3, Ag0 /WO3, and Pt0 /WO3 electrodes were used to degrade 0.35 mg L-1 progesterone hormone in aqueous solution under polychromatic irradiation for 3h. The studies on degradation were investigated under electrochemically assisted heterogeneous photocatalysis (EHP) conditions. For photodegradation of progesterone, higher performance was achieved when WO3 was functionalized and when the EHP configuration was adopted with bias at +0.7 V vs Ag/AgCl. This study reveals that incorporation of metallic NPs onto a semiconductor increases its efficiency, thereby preventing electron-hole recombination in the photocatalyst and photoelectrochemical limitations of WO3 due to surface plasmon resonance and the trapping state. Therefore, efficient advances in the degradation of organic contaminants during water treatment can be realized.

Urban area extent extraction in spaceborne HR and VHR data using multi-resolution features.

Detection of urban area extents by means of remotely sensed data is a difficult task, especially because of the multiple, diverse definitions of what an "urban area" is. The models of urban areas listed in technical literature are based on the combination of spectral information with spatial patterns, possibly at different spatial resolutions. Starting from the same data set, "urban area" extraction may thus lead to multiple outputs. If this is done in a well-structured framework, however, this may be considered as an advantage rather than an issue. This paper proposes a novel framework for urban area extent extraction from multispectral Earth Observation (EO) data. The key is to compute and combine spectral and multi-scale spatial features. By selecting the most adequate features, and combining them with proper logical rules, the approach allows matching multiple urban area models. Experimental results for different locations in Brazil and Kenya using High-Resolution (HR) data prove the usefulness and flexibility of the framework.

Protocolo de assistência a pessoas com úlceras venosas: validação de conteúdo / Assistance protocol for venous ulcers patients: validation of contents

As úlceras venosas requerem tratamento complexo e são responsáveis por morbimortalidade significativas. Este estudo objetiva identificar aspectos validados por juízes para elaboração de protocolo de assistência a pessoas com úlceras venosas. Trata-se de pesquisa descritiva, quantitativa, com 39 profissionais (30 enfermeiros, 7 médicos e 2 fisioterapeutas), realizada no Hospital Universitário Onofre Lopes, entre abril e julho/2010. A coleta de dados foi realizada através de questionário tipo check list. A análise foi feita no Statistical Package for Social Science 15.0 avaliando concordância das diretrizes. Os resultados foram os aspectos de composição do protocolo: avaliação do paciente e lesão, registro/documentação, cuidado com ferida/pele perilesional, indicação de cobertura, uso de antibiótico e tratamento da dor, tratamento cirúrgico/medicamentoso, melhoria do retorno venoso e prevenção de recidiva, encaminhamento dos pacientes, capacitação, referência/contra-referência. Conclui-se que para compor o protocolo, fazem-se necessários aspectos referentes a diagnóstico, tratamento e prevenção das lesões.