Effect of the calcium silicate and sodium phosphate remineralizing products on bleached enamel.

Context and Aims: This study evaluated the effect of calcium silicate and sodium phosphate (CSSP) dentifrice and serum on the surface of enamel bleached with hydrogen peroxide (H2O2). Materials and Methods: A total of 160 bovine enamel slabs were bleached with 35% H2O2 and treated with sodium fluoride (NaF) dentifrice-GI, CSSP dentifrice-GII; CSSP dentifrice + CSSP serum-GIII, or NaF dentifrice + NaF gel-GIV. The dentifrices were applied using a brushing machine three times daily for 7 days. After brushing, sodium phosphate gel and CSSP serum were applied. The microhardness (KNH, n = 14), surface roughness (Ra, n = 14), energy dispersive spectroscopy (n = 6), and scanning electron microscopy (n = 6) were assessed at t0 (before bleaching), t1 (after bleaching), and t2 (after postbleaching treatments). Statistical Analysis Used: The data were subjected to a two-way analysis of variance and Bonferroni's test. Results: The KNH decreased at t1 (P < 0.001) but recovered at t2 for all treatments, although only GII showed restored baseline values (P = 0.0109). The surface roughness increased at t1 (P < 0.001) and reduced at t2 (P < 0.001) for all groups, with no significant differences among groups. Enamel composition and morphology did not differ after the treatments, except for silicon accumulation in GIII. Conclusions: Postbleaching treatment with CSSP dentifrice and serum yielded superior remineralizing effects on bleached enamel.

A single exposure to sub-lethal concentrations of a glyphosate-based herbicide or fluoxetine-based agent on growth performance in Nile tilapia.

The aim of the present study was to determine e whether a single acute 96 hr exposure of a glyphosate-based herbicide (GBH) to Nile tilapia fingerlings affected growth performance during the first 90 days of culture. This association was considered as GBH increases serotoninergic activity that affect fish anorexically. Although these findings were based upon chronic investigations, this study was designed to examine whether a single, acute, but excessive concentration GBH might impair growth performance in fish. In parallel, fish were also exposed to fluoxetine (FLU), a drug that selectively inhibits the reuptake of serotonin in brain synapses, leading to increased serotoninergic activity. Data demonstrated a decreased growth performance in fingerlings exposed to GBH or FLU compared to unexposed fingerlings. In fact, FLU-exposed fingerlings exhibited lower average weight and length, diminished weight gain, which resulted in lower final biomass. GBH-exposed fish, despite displaying a lower mean body weight, exhibited a biomass similar to biomass on controls. These body weight differences were noted after 30-60- and 90-day growth period in clean water. In an aquaculture context, these observed changes may be considered harmful to the production or economic performance of large-scale farming as currently practiced in tilapia farming.

JAK/STAT as a Potential Therapeutic Target for Osteolytic Diseases.

Several cytokines with major biological functions in inflammatory diseases exert their functions through the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signal transduction pathway. JAKs phosphorylate the cytoplasmic domain of the receptor, inducing the activation of its substrates, mainly the proteins known as STATs. STATs bind to these phosphorylated tyrosine residues and translocate from the cytoplasm to the nucleus, further regulating the transcription of several genes that regulate the inflammatory response. The JAK/STAT signaling pathway plays a critical role in the pathogenesis of inflammatory diseases. There is also increasing evidence indicating that the persistent activation of the JAK/STAT signaling pathway is related to several inflammatory bone (osteolytic) diseases. However, the specific mechanism remains to be clarified. JAK/STAT signaling pathway inhibitors have gained major scientific interest to explore their potential in the prevention of the destruction of mineralized tissues in osteolytic diseases. Here, our review highlights the importance of the JAK/STAT signaling pathway in inflammation-induced bone resorption and presents the results of clinical studies and experimental models of JAK inhibitors in osteolytic diseases.

Behavioral, biochemical, and endocrine responses of zebrafish to 30-min exposure with environmentally relevant concentrations of imidacloprid-based insecticide.

The imidacloprid-based insecticides (IBIs) are among the most used insecticides worldwide, and chronic and acute toxic effects (days exposure protocols) have been reported in several species in studies of IBIs at lethal concentrations. However, there is little information on shorter time exposures and environmentally relevant concentrations. In this study, we investigated the effect of a 30-min exposure to environmentally relevant concentrations of IBI on the behavior, redox status, and cortisol levels of zebrafish. We showed that the IBI decreased fish locomotion and social and aggressive behaviors and induced an anxiolytic-like behavior. Furthermore, IBI increased cortisol levels and protein carbonylation and decreased nitric oxide levels. These changes were mostly observed at 0.013 and 0.0013 µg·L-1 of IBI. In an environmental context, these behavioral and physiological disbalances, which were immediately triggered by IBI, can impair the ability of fish to evade predators and, consequently, affect their survival.

Comportamento de idosos para a promoção da saúde: análise segundo a teoria de Nola Pender / Older adults' behavior for health promotion: analysis according to Nola Pender's theory / Comportamiento de ancianos para promover la salud: análisis según la teoría de Nola Pender

Objetivo: Analisar o comportamento de idosos em relação à promoção da saúde segundo a teoria de Nola Pender. Métodos: Pesquisa participante de abordagem qualitativa, realizada em uma Instituição de Ensino Superior de Brasília (Distrito Federal), Brasil. Os dados foram coletados ao longo de quatro encontros presenciais, por meio da estratégia de grupo de reflexão, com base em questões norteadoras especificas para cada encontro. Para a análise dos dados, utilizou-se a Teoria de Promoção da Saúde de Nola Pender que pressupõe a avaliação de características e experiências individuais, dos sentimentos e conhecimentos sobre o comportamento que se quer alcançar e do comportamento de promoção da saúde desejável. Resultados: Participaram 13 idosos, os quais revelaram que a autoestima, o trabalho e a participação em diversas atividades familiares, culturais e religiosas os ajudam a realizar ações que promovem sua saúde. Eles desejam modificar alguns comportamentos e conservar outros, em função do beneficio percebido. influências interpessoais e situações como renda, doenças, religião, escolaridade e acesso a bens e serviços são apontados como fatores que influenciam as mudanças de comportamento em saúde. Conclusão: o comportamento dos idosos revelou esforços para manter e ampliar sua saúde e graus de autonomia.

Objective: To analyze older adults' behavior in relation to health promotion according to Nola Pender's theory. Methods: This is participatory research with a qualitative approach, carried out at a Higher Education Institution in Brasília (Federal District), Brazil. Data were collected over four on-site meetings, through the reflection group strategy, based on specific guiding questions for each meeting. For data analysis, Nola Pender's Health Promotion Theory was used, which presupposes assessing individual characteristics and experiences, behavior-specific cognitions and affect and behavioral outcome. Results: The study included 13 older adults, who revealed that self-esteem, work and participation in various family, cultural and religious activities help them carry out actions that promote their health. They wish to modify some behaviors and maintain others, depending on the perceived benefit. Interpersonal influences and situations such as income, illness, religion, education and access to goods and services are highlighted as factors that influence changes in health behavior. Conclusion: Older adults' behavior revealed efforts to maintain and expand their health and degrees of autonomy.

Objetivo: Analizar el comportamiento de los ancianos frente a la promoción de la salud desde la perspectiva del modelo teórico de Nola Pender. Métodos: Estudio de investigación participante con abordaje cualitativo, con 13 ancianos. Los datos fueron recolectados a través de la estrategia de grupos de reflexión, a partir de preguntas orientadoras elaboradas por los investigadores. Se utilizó la Teoría de Enfermería de Promoción de la Salud de Nola Pender. Resultados: Hubo interrelación entre las características y vivencias individuales de los ancianos, sus sentimientos y conocimientos sobre el comportamiento deseado y el comportamiento deseado de promoción de la salud. Conclusión: Este estudio permite reflexionar sobre las condiciones y motivaciones que llevan a los ancianos a tener determinados comportamientos, que influyen en su estado de salud. Contribuciones para la práctica: Reflexión de los profesionales de la salud sobre la creación de nuevas estrategias para la implementación de acciones de promoción de la salud de la población anciana.

Exposure to levonorgestrel-based birth control pill in early life and its persistent effects in zebrafish.

The consumption of progestins has increased considerably in recent decades, as has their disposal into the environment. These substances can negatively affect the reproduction, physiology, and behavior of non-target organisms, such as fish. We aimed to evaluate the effects of exposure to environmentally relevant concentrations of levonorgestrel-control birth based (1.3, 13.3, 133, and 1330 ng/L) on the development and behavior of zebrafish (Danio rerio) in terms of mortality, hatching, spontaneous movement, and larval and adult behavioral tests. Exposure caused anxiogenic-like behavior in larvae, which persisted in adults, as demonstrated by the light-dark test. In contrast, it caused anxiolytic-like behavior in the novel tank test. There was a high mortality rate at all tested concentrations and increases in the hormone cortisol at 13.3 ng/L that affected the sex ratio. These changes may lead to an ecological imbalance, emphasizing the risk of early exposure to progestins in the environment.

In Vitro Comparison of Three Intraoral Scanners for Implant-Supported Dental Prostheses.

With continuing technological developments, there have been advances in the field of fixed prosthetics, particularly in impression-taking techniques. These technological advances mean that a wide variety of diagnostic and/or rehabilitation possibilities can be explored without the need for physical models. The aim of this study was to evaluate the accuracy of three intraoral scanners used in oral implant rehabilitation using an extraoral scanner as a reference and varying the scanning area. Three models representing different clinical scenarios were scanned 15 times by each intraoral scanner and three times by the extraoral scanner. The readings were analyzed and overlaid using engineering software (Geomagic® Control X software (Artec Europe, Luxembourg)). Statistically significant differences in accuracy were found between the three intraoral scanners, iTero® (Align Technology Inc., San Jose, CA, USA), Medit® (Medit®: Seoul, Korea), and Planmeca® (Planmeca®: Helsinki, Finland). In all clinical scenarios, the iTero® scanner had the best trueness (24.4 µm), followed by the Medit® (26.4 µm) and Planmeca® (42.1 µm). The Medit® showed the best precision (18.00 µm) followed by the iTero® (19.20 µm) and Planmeca® (34.30 µm). We concluded that the iTero® scanner had the highest reproducibility and accuracy in the clinical setting.

Lactobacillus casei reduces the extracellular matrix components of fluconazole-susceptible Candida albicans biofilms.

Fluconazole-sensitive (CaS) and -resistant (CaR) C. albicans were grown as single-species and dual-species biofilms with Lactobacillus casei (Lc) and Lactobacillus rhamnosus (Lr). Single-species Lc and Lr were also evaluated. Biofilm analysis included viable plate counts, the extracellular matrix components, biomass, and structural organization. Lc reduced the viability of CaS, water-soluble polysaccharides, and eDNA in CaS + Lc biofilm. Lc biofilm presented more eDNA than CaS. The total biomass of CaS + Lc biofilm was higher than the single-species biofilms. The viability of Lc and Lr was reduced by CaR dual-species biofilms. The total and insoluble biomass in CaS + Lr was higher than in single-species CaS biofilms. Lc hindered the growth of CaS, and their association hampered matrix components linked to the structural integrity of the biofilm. These findings allow understanding of how the implementation of probiotics influences the growth of C. albicans biofilms and thereby helps with the development of novel approaches to control these biofilms.

Construção e disponibilização de tecnologias educativas no contexto da pandemia da COVID-19: relato de experiência / Construction and availability of educational technologies in the context of the COVID-19 pandemy: experience report

Objetivo: relatar a experiência de elaborare disponibilizar tecnologias educativas para adolescentes, acerca da COVID-19. Método:Estudo descritivo, do tipo relato de experiência sobre a construção de tecnologias educativas para estudantes de uma escola da rede pública de Fortaleza-Ceará. O percurso metodológico seguiu quatro etapas: 1) Aprofundamento teórico a respeito da infecção pela COVID-19; 2) Divisão dos temas a serem abordados na elaboração das tecnologias; 3) Construção do material educativo, por meio da plataforma de designgráfico denominado "Canva"; 4) Disponibilização das tecnologias. Resultados:Foram elaborados cartazes com enfoque educativo que retrataram os seguintes temas: orientações aos cuidadores de pessoas e pacientes com suspeita ou confirmação de infecção pela COVID-19; fatores de risco e de proteção da COVID-19; controle da ansiedade durante a pandemia da COVID-19; realização de testes rápidos para diagnóstico da COVID-19; e retorno das atividades com segurança na pandemia da COVID-19. Considerações Finais:os adolescentes acessam os materiais informativos com uma abordagem educativa e comentam com familiares e professores, fato que possibilita a reflexão acerca da informação consumida e sua divulgação para outros grupos.

Objective: to report the experience of developing and providing educational technologies for adolescents, about COVID-19. Method:Descriptive study of the experience report type on the construction of educationaltechnologies for students of a public school in Fortaleza-Ceará. The methodological path follows four stages: 1) Theoretical deepening regarding the infection by COVID-19; 2) Division of themes to be defined in the technology team; 3) Construction of educational material, through the graphic design platform called "Canva"; 4) Availability of technologies. Results: Posters were created with an educational focus that portrayed the following themes: guidelines for caregivers of people and patients suspected of or infected with COVID-19; COVID-19 risk and protective factors; anxiety control during the COVID-19 pandemic; performing rapid tests for diagnosis of COVID-19; and return to activities safely in the COVID-19 pandemic. Final Considerations:teenagers access information materials with an educational approach and comment with family members and teachers, a fact that enables reflection on the information consumed and its dissemination to other groups.

Systemic inflammatory and stress markers in cattle and sheep submitted to different reproductive procedures / Marcadores sistêmicos de inflamação e estresse em bovinos e ovinos submetidos a diferentes procedimentos reprodutivos

ABSTRACT: Previous studies have evaluated the effects of different reproductive procedures on discomfort markers in sheep and cattle. Such studies may help stimulate the adoption of techniques that are more beneficial for animal welfare. However, markers that are commonly used to evaluate discomfort are highly influenced by external factors. To overcome this, several systemic markers can be evaluated to more precisely identify stress, pain, and inflammation. Such markers include cortisol, acute phase proteins, bradykinin, and substance P. We aimed to review the potential markers of stress, pain, and inflammation, and discuss how and when they are regulated after different stimuli related to reproductive procedures in cattle and sheep. Furthermore, we aimed to review how reproductive procedures with different degrees of invasiveness cause stress and provide information that may help develop strategies to limit animal discomfort.

RESUMO: Estudos anteriores avaliaram o efeito de diferentes procedimentos reprodutivos sobre marcadores de desconforto em bovinos e ovinos. Tais estudos podem estimular a adoção de técnicas que preservem o bem-estar animal. Entretanto, os marcadores comumente utilizados apresentam alta influência de fatores externos. Para contornar isso, a avaliação conjunta de diferentes parâmetros sistêmicos pode ser utilizada para determinar com maior precisão a presença de estresse, dor ou inflamação, como cortisol, proteínas de fase aguda, a bradicinina e a substância P. O objetivo desta revisão é relacionar potenciais marcadores de inflamação e estresse, discutindo como e quando são regulados frente aos estímulos em bovinos e ovinos. Ainda, pretende-se revisar de que forma procedimentos reprodutivos com diferentes graus de invasividade acarretam em desconforto, fornecendo informações para a elaboração de estratégias que possibilitem minimizá-lo.