ABSTRACT
UNLABELLED: A prospective study was conducted to determine if standardized vancomycin doses could produce adequate serum concentrations in 25 term newborn infants with sepsis. PURPOSE: The therapeutic response of neonatal sepsis by Staphylococcus sp. treated with vancomycin was evaluated through serum concentrations of vancomycin, serum bactericidal titers (SBT), and minimum inhibitory concentration (MIC). METHOD: Vancomycin serum concentrations were determined by the fluorescence polarization immunoassay technique, SBT by the macro-broth dilution method, and MIC by diffusion test in agar. RESULTS: Thirteen newborn infants (59.1%) had adequate peak vancomycin serum concentrations (20 - 40 mg/mL) and one had peak concentration with potential ototoxicity risk (>40 microg/mL). Only 48% had adequate trough concentrations (5 - 10 mg/mL), and seven (28%) had a potential nephrotoxicity risk (>10 microg/mL). There was no significant agreement regarding normality for peak and trough vancomycin method (McNemar test : p = 0.7905). Peak serum vancomycin concentrations were compared with the clinical evaluation (good or bad clinical evolution) of the infants, with no significant difference found (U=51.5; p=0.1947). There was also no significant difference between the patients' trough concentrations and good or bad clinical evolution (U = 77.0; p=0.1710). All Staphylococcus isolates were sensitive to vancomycin according to the MIC. Half of the patients with adequate trough SBT (1/8), also had adequate trough vancomycin concentrations and satisfactory clinical evolution. CONCLUSIONS: Recommended vancomycin schedules for term newborn infants with neonatal sepsis should be based on the weight and postconceptual age only to start antimicrobial therapy. There is no ideal pattern of vancomycin dosing; vancomycin dosages must be individualized. SBT interpretation should be made in conjunction with the patient's clinical presentation and vancomycin serum concentrations. Those laboratory and clinical data favor elucidation of the probable cause of patient's bad evolution, which would facilitate drug adjustment and reduce the risk of toxicity or failing to achieve therapeutic doses.
Subject(s)
Anti-Bacterial Agents/administration & dosage , Sepsis/drug therapy , Staphylococcal Infections/drug therapy , Vancomycin/administration & dosage , Drug Administration Schedule , Humans , Infant, Newborn , Microbial Sensitivity Tests , Prospective Studies , Serum Bactericidal Test , Statistics, NonparametricABSTRACT
Fifty-five infants who presented bacterial neonatal meningitis were prospectively studied to analyze the frequency and the type of sequelae. All the infants were full term newborns. There were 38 boys and 17 girls; the age of disease onset varied from 3 to 28 days. The causative organism was represented mainly by enterobacteriae. The median time of follow-up was 5 years. The frequency of neurologic sequelae was 63.7%, represented mainly by neuropsychomotor development delay (58.2%), hydrocephaly (45.5%) and convulsions (34.5%). Severe motor abnormalities ocurred in 23.6% of children (quadriplegia, diplegia, hemiparesia and ataxia). Convulsions in the acute phase of the disease and the positive cerebrospinal fluid culture were highly associated to sequelae. The school performance, obtained in 25 children, showed presence of disabilities in 48% of cases, which were significantly associated to mental retardation.
Subject(s)
Meningitis, Bacterial/complications , Female , Follow-Up Studies , Humans , Infant, Newborn , Intellectual Disability/etiology , Male , Prospective Studies , Seizures/etiologyABSTRACT
A hundred twenty full term newborns infants (RN) with ABO or Rh isoimmunization who were submitted to exchange transfusion (ET) because hyperbilirubinemia have been studied and with the procedure were demonstrated: 1. Increase the levels of serum sodium in 2.8% in the RN of ABO group and 3.2% in the RN of Rh group with a recurrence of the original values prior to the procedure within three hours post-ET. 2. The levels of serum sodium equal or superior to 180 mEq/L (mmol/L) in the donor's blood led to hypernatremia after the ET, with a recurrence of the original values prior to the procedures within three hours post-ET. 3. The levels of serum potassium of the RN decreased (-7.7% in the ABO group and -5.47% in the Rh group) with a recurrence of the normal values in the control of six hours post-ET in the ABO group and one of 12 hours in the Rh group. 4. The levels of total calcium in the controls haven't been significantly altered up to 24 hours post-ET. 5. Regarding the pH, in spite of having used blood with low levels compared to those expected for fresh blood, the RN for this experiment maintained the acid-base balance within normal range. In relation to the hematimetric values, the ET: 1. Increase both hemoglobin (Hb) and hematocrit (Hto) values in both groups (increase of 5.6% in Hb and 6% in Hto in the ABO group and increase of 9.2% in Hb and 6.1% in Hto in the Rh group), right after the ET, with a reduction in the control posterior to that. 2. The values of Hb and Hto were always inferior in the RN in the Rh group. Therefore, it has been shown the high intensity of the hemolysis in this group.
Subject(s)
Erythroblastosis, Fetal/therapy , Exchange Transfusion, Whole Blood/adverse effects , Jaundice, Neonatal/therapy , Rh Isoimmunization/therapy , ABO Blood-Group System , Female , Hemoglobins , Humans , Hypernatremia/etiology , Hypokalemia/etiology , Infant, Newborn , Male , Reference Values , Rh-Hr Blood-Group SystemABSTRACT
Fue realizado un estudio longitudinal de las principales caracteristicas bioquimicas (color, indice de color, bilirrubinas, hemoglobina, glucosa y cloruros) del LCR de 43 RNPT en el 7o. y 28o. dias de vida.Los resultados mostraron que en relacion al color, todas las muestras de LCR de los RNPT en el 7o. dia de vida fueron xantocromicas y en el 28o. dia de vida fueron incoloras en su mayoria. El indice de color fue inversamente proporcional al tiempo de vida neonatal. La bilirrubina en el 7o. dia de vida fue inversamente, proporcional a la edad gestacional, hecho no observado en el 28o. dia de vida; la hemoglobina aun estaba presente en las muestras de LCR en el 7o. y 28o. dias, aunque no teniendo valor discriminativo para la edad gestacional y para el tiempo de vida neonatal, por su baja incidencia. La glucosa y los cloruros mostraron valores semejantes independientes de edad gestacional y el tiempo de vida neonatal. Los autores comentan que los datos obtenidos para estas caracteristicas bioquimicas del LCR del RNPT, son sugestivas de mayor permeabilidad de las barreras hematocerebral y hematorraquidea
