ABSTRACT
This study was to follow IFN-γ, TNF-α and IL-10 modulation of peripheral blood mononuclear cells (PBMC) from HIV/cerebral toxoplasmosis patients (CT) during specific treatment. The results were compared with two other groups: HIV patients that had CT at least one year before (P/CT) and individuals with chronic toxoplasmosis (CHR). Blood samples (63) collected from three groups were analyzed. CT, 15 patients (3 blood samples collected one day before Toxoplasma gondii treatment; 7 and 15days during the treatment). P/CT, 5 patients (one blood sample collected at least, one year after the treatment). CHR, 13 individuals with chronic toxoplasmosis (one blood sample). Cytokine levels were assessed by ELISA after PBMC stimulation with T. gondii antigen. CT patients had low IFN-γ; discrete increase at 7th and 15th days; and the levels were recovered in cured patients (P/CT). CT patients had high TNF-α in the beginning of the treatment. TNF-α levels decrease during the treatment (7th and 15th) and in those patients who were treated (P/CT). IL-10 levels were almost similar in CT and P/CT groups but low when compared with CHR individuals. The evolution of the infection was correlated to restoration of IFN-γ response and a decrease of the inflammation. The evaluation of the immune response can provide valuable information and better monitoring of patients during specific treatment.
Subject(s)
AIDS-Related Opportunistic Infections/drug therapy , Acquired Immunodeficiency Syndrome/immunology , Interferon-gamma/blood , Interleukin-10/blood , Toxoplasmosis, Cerebral/drug therapy , Tumor Necrosis Factor-alpha/blood , AIDS-Related Opportunistic Infections/blood , AIDS-Related Opportunistic Infections/immunology , Adult , Antigens, Protozoan/immunology , Female , Humans , Leukocytes, Mononuclear/immunology , Male , Middle Aged , Prospective Studies , Toxoplasma/drug effects , Toxoplasma/immunology , Toxoplasmosis, Cerebral/blood , Toxoplasmosis, Cerebral/immunology , Young AdultABSTRACT
Toxoplasma gondii é um parasita intracelular obrigatório que pode infectar o homem e animais homeotérmicos. Nos indivíduos imunocompetentes a infecção é geralmente assintomática, os principais grupos atingidos gravemente pela doença são os indivíduos imunodeprimidos e as gestantes que adquirem a infecção durante a gestação. Neste trabalho foram analisadas 1485 amostras de soros de mulheres atendidas na rede pública de saúde da grande São Paulo, durante o período de janeiro de 2001 a julho de 2005. A detecção de anticorpos IgG anti-T.gondii foi realizada por meio de ensaio imunoenzimático(ELISA) e a técnica Imunofluorescência Indireta (IFAT) foi utilizada para detecção de anticorpos IgMe IgG anti- T.gondii. Das amostras analisadas 57,10% apresentaram anticorpos IgG anti-T.gondii, e 1,95% possuíam anticorpos específicos da classe IgM. Os inquéritos epidemiológicos realizados no Brasil demonstraram que a soroprevalência de anticorpos IgG anti-T. gondii varia de 40 a 80%, o que corroboram com os dados obtidos no presente estudo. Existe a necessidade de estudos que identifiquem as taxas de prevalência nas várias regiões brasileiras, com o intuito de implantar trabalhos de informação e conscientização da população para diminuição do risco de infecção congênita por T. gondii.
