ABSTRACT
INTRODUCTION: Cell-phones are the typical kind of object brought into the operating room from outside by hospital staff. A great effort is made to reduce the level of potentially contaminating bacteria in the operating room, and introducing these devices may run counter to good practice. The study hypothesis was that cell-phones are colonized by several strains of bacteria and may constitute a source of nosocomial contamination. The main study objective was to screen for bacterial colonies on the surfaces of cell-phones introduced in an orthopedic surgery room. The secondary objective was to assess the efficacy of decontamination. MATERIAL AND METHOD: Samples were taken from the cell-phones of hospital staff (surgeons, anaesthetists, nurses, radiology operators, and external medical representatives) entering the operating room of the university hospital center orthopedic surgery department, Toulouse (France). Sampling used Count Tact® contact gel, without wiping the phone down in advance. Both sides of the phone were sampled, before and after decontamination with a pad imbibed with 0.25% Surfanios® Premium disinfectant. A nasal sample was also taken to investigate the correlation between Staphylococcus aureus in the nasal cavities and on the cell-phone. RESULTS: Fifty-two cell-phones were sampled. Before decontamination, the mean number of colony-forming units (CFU) was 258 per phone (range, 0-1,664). After decontamination, it was 127 (range, 0-800) (P=0.0001). Forty-nine cell-phones bore CFUs before decontamination (94%), and 39 after (75%) (P=0.02). DISCUSSION: Cell-phones are CFU carriers and may thus lead to contamination. Guidelines should be drawn up to encourage cleaning phones regularly and to reduce levels of use within the operating room.
Subject(s)
Cell Phone , Decontamination , Fomites/microbiology , Operating Rooms , Staphylococcus aureus/isolation & purification , Adult , Bacteria , Colony Count, Microbial , Cross Infection/microbiology , Cross Infection/prevention & control , Female , Humans , Male , Middle Aged , Nose/microbiology , Orthopedic Procedures , Personnel, Hospital , Young AdultABSTRACT
Here we report a case of sustentorial brain abscess due to Listeria monocytogenes. Blood culture and procalcitonine blood measurement were negative. L. monocytogenes was isolated from CSF after inoculation in Castañeda medium.
Subject(s)
Brain Abscess/diagnosis , Listeria monocytogenes/physiology , Listeriosis/diagnosis , Meningitis, Listeria/diagnosis , Brain Abscess/complications , Brain Abscess/pathology , Diabetes Complications/diagnosis , Diabetes Complications/pathology , Humans , Listeria monocytogenes/growth & development , Listeria monocytogenes/isolation & purification , Listeriosis/complications , Listeriosis/pathology , Magnetic Resonance Imaging , Male , Meningitis, Listeria/complications , Meningitis, Listeria/pathology , Middle AgedABSTRACT
A technique is described which permits the direct, infrared (IR) spectrophotometric observation of peripheral leukocytes by utilizing multiple internal reflectance (MIR) spectroscopy on zinc selenide prisms. The IR spectra of nitro-blue tetrazolium (NBT) and its derivative, nitro-blue diformazan (NBF), are described and examined and these spectra are compared with those of the intracellular NBT and NBF yielding several peaks which may prove to be analytically useful. Additionally, the presence of unreduced NBT in leukocytes is demonstrated, which microscopic and chemical "NBT tests" have not previously done.
