Role of ecdysone and eyestalk factors in regulating regeneration in larval crustaceans.

Megalopae of the mud crab Rhithropanopeus harrisii can regenerate autotomized limbs. Regeneration during the megalopal stage required a significant delay in ecdysis to the first crab. Megalopae that failed to initiate regeneration complete ecdysis significantly faster than do controls. Exposure of intermolt- and premolt-stage megalopae to exogenous ecdysone significantly reduced (greater than or equal to 30%) the number of animals completing regeneration. The regeneration-induced delay in ecdysis was not significantly different in animals exposed to ecdysone. The molt cycle of nonregenerating megalopae exposed to ecdysone was further accelerated. Eyestalk ablation did not significantly reduce the molt cycle duration of regenerating animals. Eyestalkless nonregenerating animals did not display accelerated ecdysis. It is hypothesized that two additional hormonal factors are involved in "fine tuning" the molt cycle of regenerating animals. One located outside the eyestalks extends the molt cycle to accommodate regeneration. The second factor accelerates ecdysis in nonregenerating animals and appears to be produced in the eyestalks.

Inhibition of barnacle settlement and behavior by natural products from whip corals,Leptogorgia virgulata (Lamarck, 1815).

Laboratory-reared barnacle larvae were used to study natural products from whip corals. Biological assays used barnacle responses in behavioral assays and in larval settlement assays. Whip corals contained substances that were active in both assays. Substances inhibiting swimming and reversible attachment of barnacle larvae were found in an aqueous extract of whip corals. Low-molecular-weight substances inhibiting barnacle settlement were found in a methylene chloride extract of material soluble in aqueous methanol. Antisettlement activity was associated with substances with specific mobilities in several chromatography systems. Partially purified material was effective in preventing settlement at concentrations of less than 0.2 µ/ml. Settlement inhibition involves adsorption of the natural product to surfaces.

Inhibition and induction of barnacle settlement by natural products present in octocorals.

Barnacle settlement inhibitors and inducers are present in the gorgonianLeptogorgia virgulata and the pennatulaceanRenilla reniformis. The inhibitors are low-molecular-weight compounds (<20,000 daltons) that were detected in soft tissue homogenates and dialysates of homogenate and in ambient "gorgonian water." Settlement was almost completely inhibited at a dialysate concentration of 1.0 g wet weight equivalents/liter. The inhibitors probably function in chemical defense against predation and fouling, and could prove useful in ship fouling control. The settlement inducers are high-molecular-weight substances (>20,000 daltons) that adsorb to surfaces.

Free cupric ion activity in seawater: effects on metallothionein and growth in crab larvae.

Crab zoeae (Rhithropanopeus harrisii) were exposed during their development opment to a range of free cupric ion activities regulated in seawater by use of a copper chelate buffer system. Most cytosolic copper was found to be associated with metallothionein. Copper-thionein could be related to free cupric ion activity, and a shift in copper-thionein accumulation was correlated with inhibition of larval growth. These data reveal predictable relations between cupric ion activity in seawater and processes at the cellular and organismic levels.

Effects of a juvenile hormone mimic on male and female gametogenesis of the mud-crab Rhithropanopeus harrisii (Gould) (Brachyura: Xanthidae).

1. Effects of ingestion and topical application of 1.30 ppm methoprene, an insect juvenile hormone (JH) mimic, include gametogenesis disorders in both male and female mud-crab Rhithropanopeus harrisii. 2. Progressive inhibition of vitellogenesis and stimulation of spermatogenesis take place after a short exposure time (12-15 days), whereas a longer period (between 30 to 45 days) promotes inhibition of both spermatogenesis and vitellogenesis. Such modifications demonstrate the chemosterilizing action of methoprene on crustaceans. 3. Stoppage of vitellogenesis is characterized by a blockage of oocytes at terminal previtellogenesis and proliferation of the follicle cells involved in vitellogenic oocyte lysis. Spermatogenic degeneration begins with the amalgam of spermatocyte clusters in meiotic prophase and pycnosis of gonia. 4. Analogies between these results and those obtained from experiments involving androgenic gland implantations or the consequences of their hyperfunctioning are discussed.