ABSTRACT
The EDTA extract was prepared from the Leptospira interrogans serovar icterohaemorrhagiae. When inoculated subcutaneously in hamsters it conferred protection from challenge with virulent leptospires with a low dose (10 micrograms/ml) and low agglutinating antibody (40). The protection of animals obtained by the EDTA extract opens up the perspective of its use as a component of a vaccine for the control of leptospirosis.
Subject(s)
Antigens, Bacterial/immunology , Bacterial Vaccines/immunology , Leptospira interrogans/immunology , Leptospirosis/prevention & control , Lipopolysaccharides/immunology , Vaccination , Agglutinins/biosynthesis , Agglutinins/immunology , Animals , Antibodies, Bacterial/biosynthesis , Antibodies, Bacterial/immunology , Antigens, Bacterial/administration & dosage , Antigens, Bacterial/isolation & purification , Bacterial Vaccines/administration & dosage , Bacterial Vaccines/isolation & purification , Cricetinae , Dose-Response Relationship, Immunologic , Edetic Acid , Evaluation Studies as Topic , Injections, Subcutaneous , Kidney/microbiology , Leptospira interrogans/chemistry , Leptospira interrogans/classification , Leptospira interrogans/isolation & purification , Leptospirosis/immunology , Lipopolysaccharides/administration & dosage , Lipopolysaccharides/isolation & purification , Mesocricetus , SolventsABSTRACT
Serum samples were obtained from 249 individuals in Londrina-Paraná, Brazil. The sera were analysed for leptospiral agglutinins by microscopic agglutination tests and 22.1% were positive. Of these positives per group were 24.6% for butchers, 25.9% for farm workers, 17.2% for children and 22.2% for servants. The agglutinin titers ranged from 1:200 to 1:3200. The highest titers were obtained for the serovars sejroe (butchers), grippotyphosa (farm workers), javanica, canicola, panama, wolffi and pyrogenes (children) and butembo (servants).
