ABSTRACT
PURPOSE: To determine the strength of agreement between detection of caries at the margin of amalgam restorations with a caries detector dye, and detection of caries histologically utilizing light/polarizing light microscopy. MATERIALS AND METHODS: 17 permanent molars with intact amalgam restorations, determined to be caries-free by visual inspection and explorer probing, cleansed with an air/water/abrasive slurry system, and with evidence of amalgam enamel marginal staining from application of a 1% solution of sulforhodamine B caries detector dye were utilized. Longitudinal, undecalcified sections, 80-100 micrometers thick, were prepared including stained and unstained regions of the amalgam/enamel margin of each tooth, providing 34 margins for study. The sections were examined in the light/polarizing light microscope for evidence of marginal caries. The Kappa Index was calculated to determine the strength of agreement between the caries dye method and the light/polarizing light microscopic method of caries diagnosis. RESULTS: Microscopically, caries was detected in 14 of 34 margins (41%). Agreement for presence of caries was 5 of 34, and for absence of caries 8 of 34 (total agreement 38%). 12 of 17 dye-stained margins were microscopically caries-free, and 9 of 17 unstained margins had caries microscopically. Kappa Index for these findings was 0.23, indicating poor strength of agreement.
Subject(s)
Dental Caries Activity Tests , Dental Caries/diagnosis , Dental Restoration, Permanent/adverse effects , Coloring Agents , Dental Amalgam , Dental Caries/etiology , Evaluation Studies as Topic , Humans , Microscopy, Polarization , Predictive Value of Tests , Reproducibility of Results , RhodaminesSubject(s)
Denture Bases , Denture, Complete , Esthetics, Dental , Incisor , Molar , Tooth, ArtificialSubject(s)
Denture Bases , Denture Design , Denture, Complete , Esthetics, Dental , Adult , Female , Humans , Male , Middle AgedSubject(s)
Eugenol/pharmacology , Mitochondria, Liver/drug effects , Oxidative Phosphorylation/drug effects , Animals , Depression, Chemical , Dose-Response Relationship, Drug , Glutamates/metabolism , Homovanillic Acid/pharmacology , In Vitro Techniques , Isomerism , Male , Mitochondria, Liver/metabolism , Rats , Succinates/metabolismABSTRACT
In the presence of acidic phospholipids, inorganic phosphate greatly enhances the net migration of calcium ions from the aqueous phase to the organic phase, an effect that does not occur at less than the physiological pH. The calcium complex in the organic phase is shown by electron microscopy to consist of spherules, composed of stoichiometric amount of calcium, inorganic phosphate, and phospholipid. The demonstration of complex formation between calcium phosphate and acidic phospholipids adds Support to the concept that phospholipids are involved in biological mineralization.
