Thoughts and affect experienced by parents of preschool- and school-aged children during night-waking interactions.

Measures of parents' cognitions have advanced our understanding of infants' sleep. But, few comparable measures exist for use with parents of preschool- or school-age children. The Parents' night-waking thoughts and affect questionnaire (PNTQ), a self-report measure of parents' thoughts and feelings when their children wake during the night, addresses this need. This scale was evaluated in two community samples of parents (N = 473). Sample 1 included preschool-age children (2-5-years-old), and Sample 2 included preschool- and school-age children (2-10-years-old). A subsample completed 1-month test-retest reliability for the PNTQ (n = 201). Parents completed the PNTQ and measures of agreement with night-waking strategies, parenting stress, mental health, and night-waking. The psychometric properties of the PNTQ (i.e., internal consistency, test-retest reliability; content, construct, and convergent validity) were evaluated. A four-factor solution (positive thoughts about limit-setting, positive thoughts about active comforting, concerns about limit-setting, and distress about night-waking) demonstrated adequate fit in Sample 1 (robust CFI = .900; robust RMSEA = .060), which was replicated in Sample 2 (robust CFI = .870; robust RMSEA = .080). Internal consistency (αc = .68-.88) and test-retest reliability (r = .46-.80) were acceptable across subscales and samples. There was good evidence for convergent validity in both samples-including correlating with parent-reported night-waking behaviour. The PNTQ is a promising measure of thoughts and affect related to night-waking experienced by parents whose children wake during the night. The PNTQ may increase understanding of parents' use of specific night-waking strategies and may account for resistance to employing alternate techniques. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

ABCs of SLEEPING: A review of the evidence behind pediatric sleep practice recommendations.

The ABCs of SLEEPING mnemonic was developed to serve as an organizing framework for common pediatric sleep recommendations. The mnemonic stands for 1) age appropriate bedtimes and wake-times with consistency, 2) schedules and routines, 3) location, 4) exercise and diet, 5) no electronics in the bedroom or before bed, 6) positivity 7) independence when falling asleep and 8) needs of child met during the day, 9) equal great sleep. This review examines the empirical evidence behind the practices and recommendations captured by the ABCs of SLEEPING mnemonic for children aged 1 to 12. A search was conducted of key electronic databases (PubMed, PsycINFO, CINAHL, & EMBASE) to identify English articles that included the concepts of sleep, insomnia, and/or bedtime. 77 articles were eligible for inclusion and were coded to extract key details and findings regarding the relations between sleep practices identified in the ABCs of SLEEPING mnemonic and sleep outcomes. Findings provided preliminary support for many of the recommendations that are commonly made to families regarding healthy sleep practices. However, more robust investigations are needed to better understand the causal contributions of healthy sleep practices to the onset and maintenance of children's sleep problems.

Core competencies for health professionals' training in pediatric behavioral sleep care: a Delphi study.

The need to train non-sleep-specialist health professionals in evidence-based pediatric behavioral sleep care is well established. The objective of the present study was to develop a list of core competencies for training health professionals in assisting families of 1- to 10-year old children with behavioral insomnia of childhood. A modified Delphi methodology was employed, involving iterative rounds of surveys that were administered to 46 experts to obtain consensus on a core competency list. The final list captured areas relevant to the identification and treatment of pediatric behavioral sleep problems. This work has the potential to contribute to the development of training materials to prepare non-sleep-specialist health professionals to identify and treat pediatric behavioral sleep problems, ideally within stepped-care frameworks.

Barriers and facilitators of evidence-based practice in pediatric behavioral sleep care: qualitative analysis of the perspectives of health professionals.

Behavioral sleep problems are highly prevalent among young and school-aged children. Despite strong evidence for effective interventions, few children receive evidence-based care. In this study, 124 Canadian health professionals answered open-ended questions regarding barriers and facilitators of their provision of evidence-based behavioral sleep-related care, and responses were analyzed for content. Responses represented issues at an individual practice level, as well as broader systemic issues. The most frequently reported barrier and facilitator related to knowledge, training, and education. Other barriers included lack of time and institutional support, and facilitators included supportive sleep attitudes and beliefs. This study may inform the design of education programs for health professionals, and provides support for broader systems-level initiatives targeted at increasing evidence-based practice.

What do preschool-aged children do when they wake at night: toward an understanding of night-waking behaviors among community children.

Little is known about behaviors displayed by preschoolers during night-waking. Mothers (N = 203) of community preschoolers completed the Children's Night-waking Behavior Scale and measures of night-waking, co-sleeping, and daytime behavior. Approximately 1/2 of wakings involved calling out, getting out of bed, and requests for comfort; 1/4 involved activity, fear, or instrumental requests. Specific associations between night-waking behaviors, night-waking, and bedtime and daytime behaviors were observed; associations were not consistent across child gender and age. For example, comfort requests were associated with mothers' perceptions of sleep as problematic in 4- and 5-year-old children only (ρ = .42); activity requests were associated with hyperactivity for boys only (r = .36). Understanding night-waking requires consideration of factors beyond parenting, such as children's behavior, age, and gender.

Agreement with night-waking strategies among community mothers of preschool-aged children.

OBJECTIVE: To explore the night-waking schemas of mothers of preschool-aged children, using a new measure of agreement with night-waking strategies (Night-waking Vignettes Scale; NVS). METHOD: A community sample of 203 mothers (M age = 32 years, SD = 5.1) of 2- to 5-year-olds (M age = 3.4 years, SD = 1.0) provided demographic information and completed the NVS and measures of night-waking and general parenting behavior. RESULTS: Few mothers endorsed strong agreement or disagreement with limit-setting, active comforting, or rewards; mothers generally disagreed with punishment. Significant associations between agreement with night-waking strategies, child sex, and maternal educational attainment were observed; only agreement with punishment was correlated with general parenting. Agreement with night-waking strategies differed across the night-waking behaviors depicted in the NVS vignettes. Agreement with limit-setting and agreement with active comforting were correlated with night-waking. CONCLUSIONS: Mothers may be ambivalent about common night-waking strategies. Night-waking schemas appear to be complex.

Sleep problems, tiredness, and psychological symptoms among healthy adolescents.

OBJECTIVE: To examine the contribution of adolescents' sleep problems and tiredness to psychological symptoms after accounting for shared risk and psychological co-morbidity. METHODS: Secondary analyses of cross-sectional data on 12-16-year-old (N = 980) adolescents without chronic illness, functional limitation, or developmental delay. Adolescents rated sleep problems, tiredness, and psychological symptoms. Parents provided information about risk factors, adolescent tiredness, and psychological symptoms. RESULTS: Prior to accounting for psychological co-morbidity, most sleep variables were significant correlates of adolescent-, but not parent-rated, psychological symptoms. After accounting for psychological co-morbidity: nightmares were associated with adolescent-rated anxiety/depression; sleeping more than others was associated with adolescent-rated aggression; trouble sleeping was associated with adolescent-rated attention problems, anxiety/depression, and withdrawal; and adolescent-rated tiredness was associated with adolescent-rated aggression and withdrawal. CONCLUSIONS: Studies examining sleep and psychopathology should control for psychological co-morbidity.

Concurrent associations among sleep problems, indicators of inadequate sleep, psychopathology, and shared risk factors in a population-based sample of healthy Ontario children.

OBJECTIVES: Examine the contribution of sleep problems and indicators of inadequate sleep to psychopathology among children after accounting for shared risk and comorbid psychopathology. METHODS: Secondary analyses of cross-sectional data on 4- to 11-year-old (N = 1,550) children without chronic illness or developmental delay or disability. Parents provided information about sleep problems, indicators of inadequate sleep, symptoms of psychopathology, and risk factors for psychopathology. Teachers provided information about indicators of inadequate sleep and symptoms of psychopathology. RESULTS: Adjusting for risk factors and comorbid psychopathology, sleeping more than other children was related to parent-rated aggression. Nightmares and trouble sleeping were related to parent-rated anxious/depressed mood. Sleep problems were not related to attention problems. Being overtired was related to parent- and teacher-rated psychopathology. CONCLUSIONS: Relations among sleep problems, indicators of inadequate sleep, and psychopathology are complex; accounting for potential confounding variables and considering sleep variables separately may clarify these relations.

A highly flexible system for microstimulation of the visual cortex: design and implementation.

This paper presents the design of a system intended to be used as a prosthesis allowing profoundly visually impaired patients to recover partial vision by means of microstimulation in the primary visual cortex area. The main component of the system is a bio-electronic device to be implanted inside the skull of the user, composed of a plurality of stimulation modules, whose actions are controlled via an interface module. Power and data are transmitted to the implant wirelessly through a bidirectional inductive link, allowing diagnosis of the stimulating device and its environment after implantation, as well as power delivery optimization. A high level of flexibility is supported in terms of stimulation parameters, but a configurable communication protocol allows the device to be used with maximum efficiency. The core of an external controller implemented in a system on a programmable chip is also presented, performing data conversion and timing management such that phosphene intensity can be modulated by any parameter defining stimulation, either at the pulse level or in the time domain. Measured performances achieved with a prototype using two types of custom ASICs implemented in a 0.18-mum CMOS process and commercial components fulfill the requirements for a complete visual prosthesis for humans. When on/off activation is used with predefined parameters, stimuli measured on an electronic test bench could attain a rate in excess of 500 k pulses/s.

Hedgehog interacting protein in the mature brain: membrane-associated and soluble forms.

Hedgehog interacting protein (Hip) is considered as a membrane protein implicated in sequestering the hedgehog (hh) morphogens during embryonic development. Here, we demonstrate that Hip transcription also occurs in cells scattered in discrete brain areas of adult rodents and we identify the presence of membrane-associated and soluble forms of Hip in the mature brain. Moreover, we show that soluble forms of Hip, present in the conditioned medium of HEK293 cells overexpressing Hip, inhibit Sonic hedgehog (Shh)-induced differentiation of C3H10T1/2 cells, a well-characterised response associated with Shh signalling. After transfection in HEK293 cells, Hip partitions with the raft component ganglioside GM1 during density gradient centrifugation. Analysis of tagged Hip constructs reveals that the putative transmembrane domain of Hip is not cleaved suggesting that other mechanisms are implicated in the release of its soluble forms. Taken together, these data are consistent with the involvement of both membrane-associated and soluble Hip in the regulation of Shh signalling in adult neural tissues.

Towards optimization of growth via nutrient supply phasing: nitrogen supply phasing increases broccoli (Brassica oleracea var. italica) growth and yield.

A greenhouse experiment on broccoli (Brassica oleracea var. italica, cvs Windsor and Arcadia) was carried out in order to demonstrate that supplying nitrogen (N) to meet the nitrogen demands of plant growth stages, through N phasing, improves plant growth and yield, as compared to fertilizing at the conventional, optimal, constant N rate. Two broccoli cultivars and two rates of starter nitrogen fertilizer (optimum, 250 mg l(-1) and sub-optimum, 150 mg l(-1)), were combined with three timings of fertigation change. Shifting N rate, at 60% and 75% of the market plant growth cycle significantly increased shoot dry weight and head fresh weight, compared to the constant-N rates treatments (controls). The highest yield and shoot dry weight were obtained when the N-rate was switched from the optimum level (250 mg l(-1)) to the sub-optimum level (150 mg l(-1)) at inflorescence initiation. The nitrogen-to-growth-stage-fitness effect was determined and partitioned into rate effect and phasing effect. The phasing effect was greatest, on both shoot dry weight and head fresh weight, at inflorescence initiation, and subsequently decreased until harvest time. None of the interactions was significant. The results demonstrated the superiority of nitrogen supply phasing over the conventional fixed-rate-supply method.

Activin type II receptors in embryonic dorsal root ganglion neurons of the chicken.

Activin induces neuropeptide expression in chicken ciliary ganglion neurons. To determine if activin might also influence neuropeptide expression in developing sensory neurons, we examined whether type II activin receptors are expressed during embryonic development of the chicken dorsal root ganglia (DRG), and also examined the effects of activin on neuropeptide expression in cultured DRG neurons. Using reverse transcription polymerase chain reaction (rtPCR), we detected mRNAs for both the activin receptors type IIA (ActRIIA) and type IIB (ActRIIB) in DRG from embryonic day 7 through posthatch day 1. With in situ hybridization, we found that morphologically identifiable neurons express mRNAs for both ActRIIA and ActRIIB. With developmental age, a subset of neurons that hybridizes more intensely with riboprobes to these receptor mRNAs becomes evident. A similar pattern of expression is observed with immunocytochemical staining using antisera against activin type II receptors. To examine whether embryonic DRG cells respond to activin we treated dissociated cultures of DRG with activin A and assessed the expression of vasoactive intestinal peptide (VIP) and calcitonin gene related peptide (CGRP) mRNAs using semiquantitative rtPCR. Activin treatment results in an increase in VIP mRNA, but does not affect CGRP mRNA levels. These observations indicate that neurons in the embryonic chicken DRG can respond to activin and suggest that activin has the potential to play a role in the development and function of DRG sensory neurons.

Synergy of activin and ciliary neurotrophic factor signaling pathways in the induction of vasoactive intestinal peptide gene expression.

Activin, a member of the transforming growth factor-beta superfamily, can regulate neuropeptide gene expression in the nervous system and in neuroblastoma cells. Among the neuropeptide genes whose expression can be regulated by activin is the gene encoding the neuropeptide vasoactive intestinal peptide (VIP). To investigate the molecular mechanisms by which activin regulates neuronal gene expression, we have examined activin's regulation of VIP gene expression in NBFL neuroblastoma cells. We report here that NBFL cells respond to activin by increasing expression of VIP mRNA. Activin regulates VIP gene transcription in NBFL cells through a 180-bp element in the VIP promoter that was previously characterized to be necessary and sufficient to mediate the induction of VIP by the neuropoietic cytokines and termed the cytokine response element (CyRE). We find that the VIP CyRE is necessary and sufficient to mediate the transcriptional response to activin. In addition, ciliary neurotrophic factor (CNTF), a neuropoietic cytokine, synergizes with activin to increase VIP mRNA expression and transcription through the VIP CyRE. Mutations in either the Stat (signal transducer and activator of transcription) or AP-1 sites within the CyRE that reduce the response to CNTF, also reduce the response to activin. However, mutating both the Stat and AP-1 sites within the wild-type CyRE, while reducing the separate responses to either activin or CNTF, eliminates the synergy between them. These data suggest that activin and CNTF, two factors that appear to signal though distinct pathways, activate VIP gene transcription through a common transcriptional element, the VIP CyRE.