ABSTRACT
OBJECTIVES: To assess the performance of the rapid syndromic BioFire® Joint Infection Panel (BF-JIP) to detect bacterial and fungal pathogens, as well as antibiotic resistance genes, directly in synovial fluid specimens collected from patients with acute arthritis. METHODS: The study was conducted in six French bacteriological laboratories. To assess the performances of BF-JIP, results were compared with those of synovial fluid 14-day culture and, in case of discrepancy, with those of complementary molecular methods and intraoperative samples. A total of 308 synovial fluid specimens were tested after collection from 308 adults and children presenting with clinical and biological suspicion of acute arthritis; patients presenting with acute periprosthetic joint infection were included according to the European Bone and Joint Infection Society 2021 criteria. RESULTS: Only one specimen failed (no result). On the basis of the consolidated data, the BF-JIP was concordant with the 14-day culture in 280 (91.2%) of the 307 specimens finally included in the study. The positive percentage agreement was 84.9% (95% CI, 78.8-89.8%) and the negative percentage agreement was 100% (95% CI, 97.2-100%). The positive predictive value was extremely high (100%; 95% CI, 97.6-100%), whereas the negative predictive value was lower (82.6%; 95% CI, 75.7-88.2%), partially explained by the missing target species in the panel. DISCUSSION: The BF-JIP showed high performances to detect pathogens involved in acute arthritis.
Subject(s)
Arthritis, Infectious , Bacteria , Synovial Fluid , Humans , Prospective Studies , Male , Female , Aged , Middle Aged , Arthritis, Infectious/microbiology , Arthritis, Infectious/diagnosis , Adult , Child , Bacteria/isolation & purification , Bacteria/classification , Bacteria/genetics , Synovial Fluid/microbiology , Aged, 80 and over , France , Fungi/isolation & purification , Fungi/classification , Fungi/genetics , Adolescent , Young Adult , Child, Preschool , Acute Disease , Prosthesis-Related Infections/microbiology , Prosthesis-Related Infections/diagnosis , Sensitivity and Specificity , Arthritis/microbiology , Arthritis/diagnosisABSTRACT
We report a nosocomial case of Legionella pneumophila pneumonia caused by a serogroup 10 strain diagnosed with the Biofire® Pneumonia plus panel. Molecular investigations of the environment of the patient allowed us to identify the source of contamination.
