Structure of greenhouse gas-consuming microbial communities in surface soils of a nitrogen-removing experimental drainfield.

Septic systems represent a source of greenhouse gases generated by microbial processes as wastewater constituents are degraded. Both aerobic and anerobic wastewater transformation processes can generate nitrous oxide and methane, both of which are potent greenhouse gases (GHGs). To understand how microbial communities in the surface soils above shallow drainfields contribute to methane and nitrous oxide consumption, we measured greenhouse gas surface flux and below-ground concentrations and compared them to the microbial communities present using functional genes pmoA and nosZ. These genes encode portions of particulate methane monooxygenase and nitrous oxide reductase, respectively, serving as a potential sink for the respective greenhouse gases. We assessed the surface soils above three drainfields served by a single household: an experimental layered passive N-reducing drainfield, a control conventional drainfield, and a reserve drainfield not in use but otherwise identical to the control. We found that neither GHG flux, below-ground concentration or soil properties varied among drainfield types, nor did methane oxidizing and nitrous oxide reducing communities vary by drainfield type. We found differences in pmoA and nosZ communities based on depth from the soil surface, and differences in nosZ communities based on whether the sample came from the rhizosphere or surrounding bulk soils. Type I methanotrophs (Gammaproteobacteria) were more abundant in the upper and middle portions of the soil above the drainfield. In general, we found no relationship in community composition for either gene based on GHG flux or below-ground concentration or soil properties (bulk density, organic matter, above-ground biomass). This is the first study to assess these communities in the surface soils above an experimental working drainfield, and more research is needed to understand the dynamics of greenhouse gas production and consumption in these systems.

Nitrifying and Denitrifying Bacterial Communities in Advanced Nitrogen-Removal Onsite Wastewater Treatment Systems.

Advanced N-removal onsite wastewater treatment systems (OWTS) rely on nitrification and denitrification to remove N from wastewater. Despite their use to reduce N contamination, we know little about microbial communities controlling N removal in these systems. We used quantitative polymerase chain reaction and high-throughput sequencing targeting nitrous oxide reductase () and bacterial ammonia monooxygenase () to determine the size, structure, and composition of communities containing these genes. We analyzed water samples from three advanced N-removal technologies in 38 systems in five towns in Rhode Island in August 2016, and in nine systems from one town in June, August, and October 2016. Abundance of ranged from 9.1 × 10 to 9 × 10 copies L and differed among technologies and over time, whereas bacterial abundance ranged from 0 to 1.9 × 10 copies L and was not different among technologies or over time. Richness and diversity of -but not -differed over time, with median Shannon diversity indices ranging from 2.61 in October to 4.53 in August. We observed weak community similarity patterns driven by geography and technology in The most abundant and containing bacteria were associated with water distribution and municipal wastewater treatment plants, such as and species. Our results show that communities in N-removal OWTS technologies differ slightly in terms of size and diversity as a function of time, but not geography, whereas communities are similar across time, technology, and geography. Furthermore, community composition appears to be stable across technologies, geography, and time for .

How grazing affects soil quality of soils formed in the glaciated northeastern United States.

Historically, much of the New England landscape was converted to pasture for grazing animals and harvesting hay. Both consumer demand for local sustainably produced food, and the number of small farms is increasing in RI, highlighting the importance of characterizing the effects livestock have on the quality of pasture soils. To assess how livestock affect pasture on Charlton and Canton soils series in RI, we examined soil quality in farms raising beef cattle (Bos taurus), sheep (Ovis aries), and horses (Equus ferus caballus), using hayed pastures as a control. We sampled three pastures per livestock type and three control hayed pastures in May, August, and October 2012. Hay fields and pastures grazed by sheep had statistically significant (P < 0.001) better soil quality than pastures grazed by beef cattle or horses. This was driven by parameters including penetration resistance, bulk density, aggregate stability, and infiltration rate. Hayfields also showed higher soil quality measures than grazed pastures for organic matter content and active C. In addition, significant differences in nitrate and phosphate concentrations were observed among livestock types. Respiration and infiltration rates, pH, and ammonium concentrations, on the other hand, did not differ significantly among pasture types. When all soil quality indicators in this study were weighed equally, soil quality scores followed the order: hay > sheep > beef cattle > horses. The results of our study provide baseline data on the effect different types of livestock have on pasture soil quality in RI, which may be useful in making sound land use and agricultural management decisions.