ABSTRACT
Isochromosome of the long arm of chromosome 20 with loss of interstitial material [ider(20q)] is a variant of deletion of chromosome 20q and a rare abnormality in myelodysplastic syndrome (MDS). We studied seven cases with an ider(20q) in MDS. Fluorescence in situ hybridization (FISH) studies showed all proximal breakpoints to be consistently located in 20q11.21 band whereas distal breakpoints were variable. Amplification of HCK, TNFRSF6B and DIDO1 genes included in retained regions associated with loss of tumour suppressor genes in deleted regions could explain cell tumour progression and possibly the less favourable prognosis of ider(20q) compared with del(20q).
Subject(s)
Chromosomes, Human, Pair 20 , Isochromosomes , Myelodysplastic Syndromes/genetics , Aged, 80 and over , Chromosome Breakage , DNA-Binding Proteins/genetics , Female , Gene Amplification , Gene Deletion , Humans , In Situ Hybridization, Fluorescence/methods , Male , Middle Aged , Proto-Oncogene Proteins c-hck/genetics , Receptors, Tumor Necrosis Factor, Member 6b/geneticsABSTRACT
The present study evaluates the effectiveness of BACTEC NR-26A resin-containing medium in removing eight antibiotics from blood cultures. Peak concentrations achievable clinically were chosen. All antibiotics were prepared in fresh human blood samples. Blood samples were obtained prior to and at 15 min and 2, 24, 48, and 72 h after inoculation. Aztreonam, ceftriaxone, imipenem, teicoplanin, and ticarcillin were partially removed from blood cultures.
