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Non-alpha-hydroxylated aldehydes with evolved transketolase enzymes.
Cázares, Armando; Galman, James L; Crago, Lydia G; Smith, Mark E B; Strafford, John; Ríos-Solís, Leonardo; Lye, Gary J; Dalby, Paul A; Hailes, Helen C.
Org Biomol Chem ; 8(6): 1301-9, 2010 Mar 21.
Article in English | MEDLINE | ID: mdl-20204200

ABSTRACT

Transketolase mutants previously identified for use with the non-phosphorylated aldehyde propanal have been explored with a series of linear and cyclic aliphatic aldehydes, and excellent stereoselectivities observed.


Subject(s)
Aldehydes/chemistry , Aldehydes/metabolism , Transketolase/genetics , Transketolase/metabolism , Catalytic Domain , Escherichia coli/enzymology , Hydroxylation , Models, Molecular , Point Mutation , Stereoisomerism , Substrate Specificity , Transketolase/chemistry
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