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1.
Res Vet Sci ; 102: 1-6, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26412510

ABSTRACT

We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential to generate a functional mammary outgrowth. The aim of this study was to investigate on the immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of cell culture. CD49f(+)-cells significantly increased during the culture (p<0.01) and a similar trend was observed, even if less regular, for CD29(+) and ALDH1 positive cell populations. No difference during the culture was observed for CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative capabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion of the primitive population of adult mammary stem cells.


Subject(s)
Epithelial Cells/cytology , Immunophenotyping/veterinary , Mammary Glands, Animal/cytology , Stem Cells/cytology , Animals , Cattle , Cell Count , Female , Flow Cytometry , Mice , Multipotent Stem Cells/cytology , Phenotype
2.
Exp Cell Res ; 338(2): 245-50, 2015 Nov 01.
Article in English | MEDLINE | ID: mdl-26321394

ABSTRACT

Adult mammary stem cells have been identified in several species including the bovine. They are responsible for the development of the gland and for cyclic remodeling during estrous cycles and pregnancy. Epithelial cell subpopulations exist within the mammary gland. We and others showed previously that the Colony Forming Cell (CFC) assay can be used to detect lineage-restricted mammary progenitors. We carried out CFCs with bovine mammary cells and manually separated colonies with specific morphologies associated with either a luminal or a myoepithelial phenotype. Expression of specific markers was assessed by immunocytochemistry or by flow cytometry to confirm that the manual separation resulted in isolation of phenotipically different cells. When transplanted in recipient immunodeficient mice, we found that only myoepithelial-like colonies gave rise to outgrowths that resembled bovine mammary alveoli, thus proving that adult stem cells were maintained during culture and segregated with myoepithelial cells. After recovery of the cells from the transplanted mice and subsequent progenitor content analysis, we found a tendency to detect a higher progenitor frequency when myoepithelial-like colonies were transplanted. We here demonstrate that bovine adult mammary stem cells can be sustained in short-term culture and that they can be enriched by manually selecting for basal-like morphology.


Subject(s)
Epithelial Cells/cytology , Mammary Glands, Animal/cytology , Stem Cells/cytology , Animals , Biological Assay/methods , Biomarkers/metabolism , Cattle , Female , Humans , Mice , Mice, Inbred NOD , Mice, SCID , Phenotype
3.
Cell Reprogram ; 17(3): 211-20, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26053520

ABSTRACT

In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal production has not been investigated. Herein, we investigated the potential application of iPSCs in dairy production by generating bovine iPSCs and establishing their ability to generate mammary epithelial tissue. iPSCs were derived by retrovirus-mediated expression of murine Oct4, Sox2, Klf4, and c-Myc in mammary epithelium and dermal fibroblasts. The resulting reprogrammed cells stained positive for alkaline phosphatase and showed renewed expression of pluripotency genes, including Lin28, Rex1, Oct4, Sox2, and Nanog. In addition, injection of epithelial- or fibroblast-derived reprogrammed cells into nonobese diabetic (NOD/NOD) mice resulted in the formation of teratomas containing differentiated derivatives of the three germ layers, including cartilage, membranous ossification, stratified squamous epithelial tissue, hair follicles, neural pinwheels, and different types of glandular tissue. Finally, mammary epithelium-derived iPSCs could be induced to differentiate back to a mammary phenotype characterized by epithelial cells expressing cytokeratin 14 (CK14), CK18, and smooth muscle actin (SMA) as a result of treatment with 10 nM progesterone. This study reports for the first time the generation of iPSCs from bovine epithelial cells and demonstrates the potential of using iPSCs technology for generating bovine mammary tissue in vitro.


Subject(s)
Cell Transdifferentiation/genetics , Epithelial Cells/physiology , Induced Pluripotent Stem Cells/metabolism , Mammary Glands, Animal/cytology , Animals , Cattle , Cell Transdifferentiation/drug effects , Epithelial Cells/metabolism , Female , Gene Expression Regulation, Developmental , Induced Pluripotent Stem Cells/drug effects , Kruppel-Like Factor 4 , Kruppel-Like Transcription Factors/genetics , Mice , Mice, Inbred NOD , Octamer Transcription Factor-3/genetics , Progesterone/pharmacology , Proto-Oncogene Proteins c-myc/genetics , SOXB1 Transcription Factors/genetics , Teratoma/etiology , Transgenes
4.
Res Vet Sci ; 97(2): 367-75, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25189469

ABSTRACT

The detection and characterization of bovine mammary stem cells may give a better understanding of the cyclic characteristic of mammary gland development. In turn, this could potentially offer techniques to manipulate lactation yield and for regenerative medicine. We previously demonstrated that adult stem cells reside in the bovine mammary gland and possess an intrinsic regenerative potential. In vitro maintenance and expansion of this primitive population is a challenging task that could make easier the study of adult mammary stem cells. The aim of this study is to investigate this possibility. Different subpopulations of mammary epithelial cells emerge when they are cultured in two defined culture conditions. Specific cell differentiation markers as cytokeratin 18 (CK18) and cytokeratin 14 (CK14) were expressed with significant differences according to culture conditions. Vimentin, a well-known fibroblast marker was observed to increase significantly (P < 0.5) only after day 20. In both conditions, after prolonged culture (25 days) a subset of cells still retained regenerative capabilities. These cells were able to form organized pseudo-alveoli when transplanted in immunodeficient mice as shown by the expression of cytokeratin 14 (CK14), cytokeratin 18 (CK18), p63 (a mammary basal cell layer marker) and Epithelial Cell Adhesion Molecule (EpCAM). We also were able to observe the presence of milk proteins signal in these regenerated structures, which is a specific marker of functional mammary alveoli. Progenitor content was also analyzed in vitro through Colony-Forming Cell (CFC) assays with no substantial differences among culture conditions and time points. These results demonstrate that long-term culture of a multipotent cell subpopulation with intrinsic regenerative potential is possible.


Subject(s)
Cattle , Cell Differentiation , Epithelial Cells/cytology , Mammary Glands, Animal/cytology , Multipotent Stem Cells/cytology , Phenotype , Animal Husbandry/methods , Animals , Cells, Cultured , Epithelial Cells/metabolism , Female , Keratin-14/metabolism , Keratin-18/metabolism , Lactation/metabolism , Mammary Glands, Animal/metabolism , Mice , Mice, Inbred NOD , Mice, SCID , Multipotent Stem Cells/metabolism , Transplantation, Heterologous/veterinary
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