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Methods Mol Biol ; 1104: 395-417, 2014.
Article in English | MEDLINE | ID: mdl-24297428

ABSTRACT

The use of baculoviruses has become a standard approach in many labs for recombinant protein production. In addition to giving a broad and practical overview of the technology, this chapter focuses in particular on two recent developments in the field and how these can be efficiently exploited for protein production: the use of baculovirus-infected insect cells and in vivo recombination-mediated production of recombinant viruses.


Subject(s)
Baculoviridae/genetics , Protein Engineering/methods , Recombinant Proteins/genetics , Animals , Automation , Cell Culture Techniques/instrumentation , Cell Culture Techniques/methods , Cell Line , Insecta/virology , Sf9 Cells/virology , Transfection
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