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1.
Enzyme Microb Technol ; 128: 9-21, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31186114

ABSTRACT

Silica has been extracted from rice husks via a simple hydrothermal process and functionalized with triethoxy(octyl)silane -OCTES (Octyl-SiO2) and (3-aminopropyl)triethoxysilane - 3-APTES (Amino-SiO2), with the aim of using it as support to immobilize lipase from Thermomyces lanuginosus (TLL) via adsorption. The supports have been characterized by particle size distribution and elemental analyses, XRD, TGA, SEM, AFM and N2 physisorption so as to confirm their functionalization. Effect of pH, temperature, initial protein loading and contact time on the adsorption process has been systematically evaluated. Maximum immobilized protein loading of 12.3 ± 0.1 mg/g for Amino-SiO2 (5 mM buffer sodium acetate at pH 4.0, 25 °C and initial protein loading of 20 mg/g) and 21.9 ± 0.1 mg/g for Octyl-SiO2 (5 mM buffer sodium acetate at pH 5.0, 25 °C and initial protein loading of 30 mg/g) was observed. However, these biocatalysts presented similar catalytic activity in olive oil emulsion hydrolysis (between 630 and 645 U/g). TLL adsorption was a spontaneous process involving physisorption. Experimental data on Octyl-SiO2 and Amino-SiO2 adsorption were well-fitted to the Langmuir isotherm model. It was also investigated whether these biocatalysts could synthesize cetyl esters via esterification reaction. Thus, it was found that cetyl stearate synthesis required 100-110 min of reaction time to attain maximum conversion percentage (around 94%). Ester productivity of immobilized TLL on Amino-SiO2 was 1.3-3.1 times higher than Octyl-SiO2.


Subject(s)
Adsorption , Enzymes, Immobilized/metabolism , Eurotiales/enzymology , Lipase/metabolism , Hydrogen-Ion Concentration , Hydrolysis , Kinetics , Lipase/isolation & purification , Olive Oil/metabolism , Oryza/chemistry , Silicon Dioxide/isolation & purification , Silicon Dioxide/metabolism , Temperature
2.
Int J Biol Macromol ; 120(Pt B): 2354-2365, 2018 Dec.
Article in English | MEDLINE | ID: mdl-30179692

ABSTRACT

Ion-exchange supports have been prepared via sequential functionalization of silica-based materials with (3­Glycidyloxypropyl)trimethoxysilane (GPTMS) (Epx-SiO2) and activation with glycine (Gly-Epx-SiO2) in order to immobilize lipase from Thermomyces lanuginosus (TLL) via adsorption. Rice husk silica (RHS) was selected as support with the aim of comparing its performance with commercial silica (Immobead S60S). Sequential functionalization/activation of SiO2-based supports has been confirmed by AFM, SEM and N2 adsorption-desorption analyses. Maximum TLL adsorption capacities of 14.8 ±â€¯0.1 mg/g and 16.1 ±â€¯0.6 mg/g using RHS and Immobead S60S as supports, respectively, have been reached. The Sips isotherm model has been used which was well fitted to experimental data on TLL adsorption. Catalytic activities of immobilized TLL were assayed by olive oil emulsion hydrolysis and butyl stearate synthesis via an esterification reaction. Hydrolytic activity of the biocatalyst prepared with a commercial support (357.6 ±â€¯11.2 U/g) was slightly higher than that of Gly-Epx-SiO2 prepared with RHS (307.4 ±â€¯7.2 U/g). On the other hand, both biocatalysts presented similar activity (around 90% conversion within 9-10 h of reaction) and reusability after 6 consecutive cycles of butyl stearate synthesis in batch systems.


Subject(s)
Biocatalysis , Enzymes, Immobilized/metabolism , Epoxy Compounds/chemistry , Eurotiales/enzymology , Glycine/chemistry , Lipase/metabolism , Silicon Dioxide/chemistry , Enzymes, Immobilized/chemistry , Esterification , Hydrogen-Ion Concentration , Hydrolysis , Ion Exchange , Lipase/chemistry , Stearates/chemistry
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