ABSTRACT
This research was developed in order to demonstrate the tissue engineering method as an alternative to conventional methods for bone reconstruction, in order to overcome the frequent failures of alloplastic commercial biomaterials, allografts and autografts. Tissue engineering is an in vitro method used to obtain cell based osteoinductive bone grafts. This study evaluated the feasibility of creating tissue-engineered bone using mesenchymal cells seeded on a scaffold obtained from the deciduous red deer antler. We have chosen mesenchymal stem cells because they are easy to obtain, capable to differentiate into cells of mesenchymal origin (osteoblasts) and to produce tissue such as bone. As scaffold, we have chosen the red deer antler because it has a high level of porosity. We conducted a case control study, on three groups of mice type CD1--two study groups (n=20) and a control group (n=20). For the study groups, we obtained bone grafts through tissue engineering, using mesenchymal stem cells seeded on the scaffold made of deciduous red deer antler. Bone defects were surgically induced on the left parietal bone of all subjects. In the control group, we grafted the bone defects with commercial biomaterials (OsteoSet, Wright Medical Technology, Inc., Arlington, Federal USA). Subjects were sacrificed at two and four months, the healing process was morphologically and histologically evaluated using descriptive histology and the golden standard - histological scoring. The grafts obtained in vivo through tissue engineering using adult stem cell, seeded on the scaffold obtained from the red deer antler using osteogenic medium have proven their osteogenic properties.
Subject(s)
Biocompatible Materials/pharmacology , Bone Substitutes/pharmacology , Face , Plastic Surgery Procedures , Skull , Tissue Engineering , Animals , Biocompatible Materials/chemistry , Bone Regeneration/physiology , Bone Substitutes/chemistry , Bone Transplantation/methods , Cells, Cultured , Commerce , Face/surgery , Mice , Plastics/chemistry , Plastics/pharmacology , Skull/surgery , Tissue Engineering/methods , Tissue Scaffolds/chemistryABSTRACT
UNLABELLED: Life quality has a major importance in the actual social context. The evaluation af the pain sensation as a stress major factor is difficult regarding the variety of the methods, their relevance and their compliance. MATERIAL AND METHOD: We've made an evaluation concerning the trigeminal essential neuralgia pain using different tipe of parameters. RESULTS: The results obtained proved a scientific and clinical value of the method that we've initiated and applied in these research.
Subject(s)
Oral Health , Quality of Life , Trigeminal Neuralgia/diagnosis , Adult , Aged , Aged, 80 and over , Analgesics, Non-Narcotic/therapeutic use , Carbamazepine/therapeutic use , Case-Control Studies , Humans , Middle Aged , Pain Measurement/methods , Psychometrics , Statistics, Nonparametric , Treatment Outcome , Trigeminal Neuralgia/psychology , Trigeminal Neuralgia/therapyABSTRACT
UNLABELLED: Laser-therapy opened new perspectives for the treatment of vascular lesions of the maxillofacial regions. MATERIAL AND METHOD: The study evaluated the results of diode laser percutaneous coagulation of oral and maxillofacial hemangioma. A group of 12 patients with sonographically certified hemangioma was treated using percutaneous coagulation with a diode laser. RESULTS: The results showed good and rapid healing, without any complications. All patients presented size reduction of the lesions, a favorable esthetic appearance and no morbidity. There were no complications over a follow-up period of 3 years. The study recommends diode laser-therapy as a valuable treatment option for hemangioma in correctly selected indications.
