Image Analysis of Adventitious Root Quality in Wild Sage and Glossy Abelia Cuttings after Application of Different Indole-3-Butyric Acid Concentrations.

Adventitious root (AR) formation is a key step in stem cutting propagation of economically important woody ornamentals. Inadequate environmental and hormonal conditions can lead to the production of an insufficient or modest number of ARs in stem cutting, with a consequent decrease in quality. The aim of this research was to optimize wild sage and glossy abelia autumn stem cutting propagation protocols, using image analysis to assess the effects of different IBA concentrations and cultivars on AR quality. For both taxa, the treatments were: four IBA concentrations: 0, 1250, 2500 and 5000 mg L-1 and two cultivars: 'Little Lucky' (cv1) and 'Yellow' (cv2) from Lantana, and 'Canyon Creek' (cv1) and 'Eduard Goucher' (cv2) from Abelia. Results show that IBA application is not needed to enhance rooting ability; however, IBA concentration is an important factor determining the best overall AR quality in both taxa. In wild sage applying 5000 mg L-1 IBA improved AR quality in 'Little Lucky', increasing the root number, total length, surface area and number of forks and crossings, but decreased quality in 'Yellow'. In glossy abelia 'Edouard Goucher', 5000 mg L-1 IBA increased the root number, but 1250 mg L-1 IBA improved AR quality; 'Canyon Creek' did not perform as well as cv2 at these concentrations. This study confirms that sensitivity to IBA dosage varies among species and their cultivars. Findings may help the commercial nursery industry produce higher quality cuttings.

Petunia Performance Under Application of Animal-Based Protein Hydrolysates: Effects on Visual Quality, Biomass, Nutrient Content, Root Morphology, and Gas Exchange.

Sustainable plant production practices have been implemented to reduce the use of synthetic fertilizers and other agrochemicals. One way to reduce fertilizer use without negatively impacting plant nutrition is to enhance crop uptake of nutrients with biostimulants. As the effectiveness of a biostimulant can depend on the origin, species, dose, and application method, the aim of this research was to evaluate the effect of a commercial animal-based protein hydrolysate (PH) biostimulant on the visual quality, biomass, macronutrient content, root morphology, and leaf gas exchange of a petunia (Petunia × hybrida Hort. "red") under preharvest conditions. Two treatments were compared: (a) three doses of an animal-based PH biostimulant: 0 (D0 = control), 0.1 (D0.1 = normal), and 0.2 g L-1 (D0.2 = high); (b) two biostimulant application methods: foliar spray and root drenching. The dose × method interaction effect of PH biostimulant on the plants was significant in terms of quality grade and fresh and dry biomass. The high dose applied as foliar spray produced petunias with extra-grade visual quality (number of flowers per plant 161, number of leaves per plant 450, and leaf area per plant 1,487 cm2) and a total aboveground dry weight of 35 g, shoots (+91%), flowers (+230%), and leaf fresh weight (+71%). P and K contents were higher than in untreated petunias, when plants were grown with D0.2 and foliar spray. With foliar spray at the two doses, SPAD showed a linear increase (+21.6 and +41.0%) with respect to untreated plants. The dose × method interaction effect of biostimulant application was significant for root length, projected and total root surface area, and number of root tips, forks, and crossings. Concerning leaf gas exchange parameters, applying the biostimulant at both doses as foliar spray resulted in a significant improvement in net photosynthesis (D0.1: 22.9 µmol CO2 m-2 s-1 and D0.2: 22.4 µmol CO2 m-2 s-1) and stomatal conductance (D0.1: 0.42 mmol H2O m-2 s-1 and D0.2: 0.39 mmol H2O m-2 s-1) compared to control. These results indicate that application of PH biostimulant at 0.2 g L-1 as foliar spray helped to achieve extra-grade plants and that this practice can be exploited in sustainable greenhouse conditions for commercial production of petunia.

Evaluation of Glycosyl-Hydrolases, Phosphatases, Esterases and Proteases as Potential Biomarker for NaCl-Stress Tolerance in Solanum lycopersicum L. Varieties.

Salinity stress limited the production in over 30% of irrigated crops and 7% of dryland agriculture worldwide. The objective was to evaluate the effects of NaCl-stress on the enzymatic activity in tomato. Two experiments were carried out in germination and early vegetative growth stages. The activity of proline and peroxidase of eight varieties (Missouri, Yaqui, Vita, Feroz, Rio Grande, Tropic, Ace, and Floradade) submitted to NaCl concentrations (0, 50, 100, 150 and 200 mM de NaCl) and the semi-quantitative activity of 19 enzymes APY ZYM® were measured under a completely randomized design with four replications. Data were analyzed using univariate-multivariate analysis of variance, Tukey's HSD (p = 0.05), canonical discriminant and cluster analysis. The results showed significant differences between varieties and NaCl in proline content. Proline increased as the NaCl concentration increased. Peroxidase did no show significant differences. Eight enzymes were included within the model to properly classify the varieties and NaCl. In shoots, varieties and NaCl showed that enzymatic activity was higher in the order of alkaline-phosphatase > leucine arylamidase > acid phosphatase > naphthol-AS-BI-phosphohydrolase > n-acetyl-ß-glucosaminidase > ß-galactosidase, while in roots was higher in the order of alkaline-phosphatase > naphthol-AS-BI-phosphohydrolase > acid phosphatase > n-acetyl-ß-glucosaminidase. Acid and alkali phosphatase, lipase, esterase, ß-galactosidase, and trypsin can be a potential biomarker for NaCl-stress tolerance in tomato.

Effects of an Animal-Derived Biostimulant on the Growth and Physiological Parameters of Potted Snapdragon (Antirrhinum majus L.).

To assess the effect a new animal-derived biostimulant on the growth, root morphology, nitrogen content, leaf gas exchange of greenhouse potted snapdragon, three treatments were compared: (a) three doses of biostimulant (D): 0 (D0 or control), 0.1 (D0.1), and 0.2 g L-1 (D0.2); (b) two biostimulant application methods (M): foliar spray and root drenching; (c) two F1Antirrhinum majus L. hybrids (CV): "Yellow floral showers" and "Red sonnet." The treatments were arranged in a randomized complete-block design with four replicates, with a total of 48 experimental units. Plant height (+11%), number of shoots (+20%), total shoot length (+10%), number of leaves (+33%), total leaf area (+29%), and number of flowers (+59%) and total aboveground dry weight (+13%) were significantly increased by the biostimulant application compared to the control, regardless of the dose. The lowest dose resulted in the best effect on the ground plant dry weight (+38%) and, in order to the root system, on total length (+55%), average diameter (+36%), volume (+66%), tips (+49%), crossings (+88%), forks (+68%), projected (+62%), and total surface area (+28%). Compared to the control, plants treated with the biostimulant significantly enhanced leaf (+16%) and root (+8%) nitrogen content, photosynthetic rate (+52%), transpiration rate (+55%), and stomatal conductance (+81%), although there were no changes in dark-adapted chlorophyll fluorescence. Differences in the application method were not evident in the aboveground morphological traits, except in the plant shoot number (root drenching: +10%). The foliar spray compared to root drenching had a significant effect only on flower dry weight (3.8 vs. 3.0 g plant-1). On the other hand, root drenching had a positive effect on ground dry weight (2.7 vs. 2.3 g plant-1), root morphology, leaf-N and root-N content (+3%), transpiration rate (+21%), stomatal conductance (+40%), concentration of CO2 in intracellular spaces (+11%), as well as on the efficiency of Photosystem II (+11%). A higher pot quality was obtained in "Red sonnet" compared to "Yellow floral shower." Based on our findings, applying the biostimulant to potted snapdragon at the lowest dose, as part of a fertilizing regime, improves the crop quality in an agro-environmental sustainable way.

Response of Weeping Lantana (Lantana montevidensis) to Compost-Based Growing Media and Electrical Conductivity Level in Soilless Culture: First Evidence.

The most common substrate for potted ornamental plants is prepared with Sphagnum peat; however, the cost and declining availability of high-quality peat, due to environmental constraints, make it necessary to investigate for alternative organic materials. The present study aimed to determine the effects of partial compost replacement with peat and the optimum electrical conductivity (EC) level of the nutrient solution in potted weeping lantana [L. montevidensis (Spreng.) Briq.] under a recirculating soilless system. Three compost-based substrates were prepared by mixing peat (Pe) with sewage sludge-based compost (Co.) at a rate of 0% (Pe90Co0Pu10, control), 30% (Pe60Co30Pu10), or 60% (Pe30Co60Pu10), respectively. The soilless recirculated closed system was equipped with two different EC levels (high and low) of nutrient solution. Growing media main characteristics and plant bio-morphometric parameters were evaluated. Our first evidence clearly demonstrates that the replacement of peat with compost at doses of 30% and 60% gave the poorest results for plant diameter, shoots, leaves, flowers, and fresh and dry mass, probably indicating that the physical characteristics of the compost based substrates may be the major factor governing plant growth rate. Compost media pH and EC values, too, showed negative effects on plant growth. Considering the effect of EC level, all morphological traits were significantly improved by high EC compared to low EC in weeping lantana. Thus, based on first evidence, further research is needed on organic materials for the establishment of ecological substrates with optimal physicochemical characteristics for the growth of weeping lantana.

Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)-A Review.

Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

Monitoring and predicting the risk of violence in residential facilities. No difference between patients with history or with no history of violence.

BACKGROUND: Most people with mental disorders are not violent. However, the lack of specific studies in this area and recent radical changes in Italy, including the closure of six Forensic Mental Hospitals, has prompted a more detailed investigation of patients with aggressive behaviour. AIMS: To compare socio-demographic, clinical and treatment-related characteristics of long-term inpatients with a lifetime history of serious violence with controls; to identify predictors of verbal and physical aggressive behaviour during 1-year follow-up. METHODS: In a prospective cohort study, patients living in Residential Facilities (RFs) with a lifetime history of serious violence were assessed with a large set of standardized instruments and compared to patients with no violent history. Patients were evaluated bi-monthly with MOAS in order to monitor any aggressive behaviour. RESULTS: The sample included 139 inpatients, 82 violent and 57 control subjects; most patients were male. The bi-monthly monitoring during the 1-year follow-up did not show any statistically significant differences in aggressive behaviour rates between the two groups. The subscale explaining most of the MOAS total score was aggression against objects, although verbal aggression was the most common pattern. Furthermore, verbal aggression was significantly associated with aggression against objects and physical aggression. CONCLUSIONS: Patients with a history of violence in RFs, where treatment and clinical supervision are available, do not show higher rates of aggressiveness compared to patients with no lifetime history of violence. Since verbal aggression is associated with more severe forms of aggression, prompt intervention is warranted to reduce the risk of escalation.

Compositional Analysis of Lavandula pinnata Essential Oils.

The genus Lavandula includes about thirty species plus a number of intraspecific taxa and hybrids, which are distributed in the Mediterranean area. The traditional use of lavender both as perfume or medicinal plant is known since antiquity. Nowadays several species are extensively cultivated for the extraction of their essential oils (EOs) which are used in manufactured products like cosmetics and perfumes or in phytotherapy. Lavandula pinnata L. f. (syn L. pinnata Lundmark) is a rare species native to the Canary Islands used in folk medicine as relaxant and also a valuable remedy against bites. To the best of our knowledge, EOs from L. pinnata have been very little studied. The present paper reports on the quali- and quantitative compositional profile of the EOs distilled (by a Spring type apparatus) from the aerial parts (flowers and leaves) of this species cultivated in soilless conditions. Chemical analyses by means of GC and GC-MS techniques have indicated that oxygenated monoterpenes are the main constituents of both the flowers (68.30%) and the leaves (83.65%). Carvacrol is the main compound which characterizes the EOs of this species. In addition, discrete amounts of spathulenol (12.22%) and caryophyllene oxide (14.62%) have been detected in flowers EOs, while leaves EOs contained small amounts of carvacrol methyl ether (2.52%).