Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 39
Filter
1.
Surgeon ; 14(2): 63-8, 2016 Apr.
Article in English | MEDLINE | ID: mdl-24947501

ABSTRACT

BACKGROUND: Rapid maxillary expansion (RME) is used to expand the narrow maxilla. Dental and skeletal affects have previously been reported but few studies have reported on the overlying soft tissue changes. This study reports on the immediate effects of RME on the naso-maxillary facial soft tissue using 3D stereophotogrammetry. METHODS: Fourteen patients requiring upper arch expansion using RME as part of their full comprehensive orthodontic plan were recruited. Cone beam CT scans and stereophotogrammetry images were taken for each patient; pre-RME activation (T0) and immediately post-RME expansion (T1). Based on twenty-three landmarks, 13 linear and 3 angular measurements were made from each of the stereophotogrammetry images. A linear measurement at ANS was taken from each CBCT image. Using a Wilcoxon signed rank test, the pre-RME and post-RME measurements were compared. RESULTS: The mean separation of the anterior nasal spine was 3.8 mm ± 1.2 mm. The largest median increase was in nasal base width (1.6 mm), which was statistically significant (p = 0.001). Changes in the nasal dorsum height, nasal tip protrusion, philtrum width, and upper lip length were not statistically significant (p < 0.05). No significant differences were observed in the nostril linear measurements, expect for columella width (p = 0.009). Naso-labial angle decreased but was not statistically significant (p = 0.276). The only statically significant angular change was an increase in the nasal tip displacement angle (p = 0.001). CONCLUSION: Rapid maxillary expansion produces subtle changes in the naso-maxillary soft tissue complex. There is an increase in nasal base width, retraction and flattening of the nasal tip. These changes are small, less than 2 mm and variable between patients.


Subject(s)
Imaging, Three-Dimensional , Maxilla , Nasal Bone , Palatal Expansion Technique , Palate , Photogrammetry/methods , Child , Cone-Beam Computed Tomography , Female , Follow-Up Studies , Humans , Male , Retrospective Studies
2.
Surgeon ; 13(3): 132-8, 2015 Jun.
Article in English | MEDLINE | ID: mdl-24325933

ABSTRACT

BACKGROUND: The transverse skeletal effects of rapid maxillary expansion (RME) have previously been assessed using cone-beam CT (CBCT). However, to date the majority of studies assess the changes based on two-dimensional slice images, which under utilises the three-dimensional (3D) data captured. This study optimizes the volumetric CBCT data by generating 3D rendered surface models to quantity and visualize the immediate 3D changes of the mid-facial bone surfaces following RME. METHODS: The sample consisted of 14 patients who required RME prior to fixed appliances. Pre-treatment (T0) and immediate post expansion (T1) CBCT images were taken. Following superimposition the mid face was divided into six anatomical regions. A one-sample t-test was used to determine if the differences between the two surfaces were significantly ≥0.5 mm. FINDINGS: All regions showed a change following RME ≥ 0.5 mm. The maxillary and nasal bones showed 2.3 mm and 2.4 mm expansion respectively, followed by the zygomatic bones (1.4 mm), 2 cases showing asymmetric expansion. CONCLUSIONS: The use of 3D surface rendered models allows quantification and visualisation of 3D changes in the mid-facial skeleton at anatomical sites distant of RME activation. Following activation there can be a pan mid-facial expansion, including not only the maxilla but also the nasal lateral bones and zygomas. The response was highly variable and asymmetric expansion can occur.


Subject(s)
Cone-Beam Computed Tomography , Facial Bones/diagnostic imaging , Facial Bones/surgery , Palatal Expansion Technique , Adolescent , Child , Computer Simulation , Female , Humans , Imaging, Three-Dimensional , Male , Maxilla/diagnostic imaging , Maxilla/surgery , Nasal Bone/diagnostic imaging , Nasal Bone/surgery , Zygoma/diagnostic imaging , Zygoma/surgery
3.
Theriogenology ; 78(6): 1361-70, 2012 Oct 01.
Article in English | MEDLINE | ID: mdl-22898028

ABSTRACT

The objective was to evaluate the efficacy of domperidone in the prevention of reproductive complications of fescue toxicosis in periparturient mares. Pregnant mares at ≤310 days of gestation were fed ≥200 µg ergovaline per kg diet daily in endophyte-infected fescue hay and seed, starting ≥30 days before their expected foaling date (EFD: 340 days after breeding). Thirty-five mares were randomized to a treatment group to receive either domperidone gel (n = 20, 1.1 mg/kg, PO, once daily) or placebo (n = 15). Treatment was initiated 10 to 15 days before the EFD and continued for 5 days after foaling. "Treatment success" was defined as foaling within 14 days of the EFD, adequate mammary development on the day of foaling, and adequate lactation for 5 days postpartum. Twenty-seven mares were included in the effectiveness analysis. More mares in the domperidone group (12/13, P < 0.0001) were treatment successes than in the control group (1/14). Gestation length was shorter (P = 0.0011), and lactation at foaling (P = 0.0011) was better for the domperidone-group mares. Foals from two control mares were born dead and four others died or were euthanized within a few days after birth, compared with one foal death (an autolyzed twin) from a domperidone-treated mare. Plasma IgG concentrations were evaluated in 24 foals. Failure of passive transfer of immunoglobulins (IgG <800 mg/dL) occurred in 13/16 (81%) foals of domperidone-group mares and 7/8 (88%) foals of control mares. In conclusion, the reproductive complications of fescue toxicosis in periparturient mares induced by a fescue seed/hay model were prevented by treatment with domperidone.


Subject(s)
Domperidone/administration & dosage , Ergotism/veterinary , Horse Diseases/prevention & control , Neotyphodium/metabolism , Poaceae/microbiology , Pregnancy Complications/veterinary , Animals , Ergotamines , Ergotism/complications , Ergotism/prevention & control , Female , Festuca/microbiology , Gels , Gestational Age , Horse Diseases/chemically induced , Horses , Lactation , Lolium/microbiology , Parturition , Pilot Projects , Placebos , Pregnancy , Pregnancy Complications/chemically induced , Pregnancy Complications/prevention & control , Treatment Outcome
4.
J Anim Sci ; 81(10): 2568-74, 2003 Oct.
Article in English | MEDLINE | ID: mdl-14552385

ABSTRACT

The aim of this study was to evaluate the effect of a dopamine antagonist, domperidone, in nonpregnant, reproductively cycling heifers consuming endophyte-infected (EI) fescue diets. Thirty crossbred heifers (Angus x Holstein or Hereford x Holstein) were assigned to one of three treatment groups (n = 10); endophyte-free (EF) fescue diet, EI fescue diet, or endophyte-infected diet and treated with domperidone (EID). Heifers fed EI diets had decreased weight gains compared with heifers fed EF or EID (P < 0.05) during a 21-d treatment period. Ovarian structures were monitored via transrectal ultrasound to determine follicle size and day of ovulation. Blood plasma samples were collected daily and analyzed for progesterone concentration to determine luteal function. Heifers ingesting EI diets had estrous cycles of shorter duration and lower mid-cycle progesterone concentrations than heifers in the EF or EID treatments (P < 0.05). Ovaries from a subset of heifers in each group (n = 3 per group) were harvested and in vitro secretion of progesterone from luteal tissue extracts was determined. No differences in progesterone concentrations were detected among luteal tissue incubates (P > 0.05). These results suggest that domperidone supplementation of heifers consuming EI fescue may ameliorate certain symptoms of fescue toxicosis.


Subject(s)
Cattle/physiology , Domperidone/pharmacology , Dopamine Antagonists/pharmacology , Poaceae/microbiology , Reproduction/drug effects , Weight Gain/drug effects , Acremonium , Animal Feed/adverse effects , Animals , Cattle/blood , Estrus/drug effects , Estrus/physiology , Female , Food Contamination , Ovary/diagnostic imaging , Ovary/drug effects , Progesterone/blood , Random Allocation , Ultrasonography
5.
Eur J Orthod ; 22(5): 519-28, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11105408

ABSTRACT

The purpose of this study was to compare the transverse dimensions of skeletal, dental, and nasal structures of a group of patients with maxillary narrowness before and after rapid maxillary expansion (RME) with an untreated control group using postero-anterior (PA) cephalometric radiographs. The material consisted of PA cephalograms of 25 children with a posterior crossbite (mean age 13 years 4 months), and 25 age- and sex-matched controls (mean age 13 years 11 months). Both groups consisted of 20 females and five males. Thirty-four reference points were digitized using the Dentofacial Planner software program. The 17 variables studied consisted of six skeletal, four dental, and seven intra-nasal linear measurements. Student's t-tests were used to compare the differences between the groups, and the effect of RME on skeletal, dental, and nasal structures. RME produced small, but statistically significant changes in maxillary width, upper and lower molar widths, the width between upper central incisor apices, and intra-nasal width. When compared with previous studies, the changes observed were similar for patients of a similar age group, but less than reported for a younger population. There is some evidence that the pattern of expansion produced by RME will vary depending on the age and maturity of the subject.


Subject(s)
Cephalometry , Malocclusion/therapy , Maxillofacial Development , Palatal Expansion Technique , Adolescent , Case-Control Studies , Child , Female , Humans , Male , Maxilla/anatomy & histology , Molar/anatomy & histology , Nasal Cavity/anatomy & histology , Odontometry , Radiographic Image Enhancement
6.
Phys Ther ; 80(6): 621, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10842413
7.
J Reprod Fertil Suppl ; (56): 185-93, 2000.
Article in English | MEDLINE | ID: mdl-20681130

ABSTRACT

The aim of the present study was to determine the effect of prolonged administration of the dopamine antagonist domperidone on follicular development, ovulation and endocrine profiles in anoestrous mares. Anoestrous mares (n=16) were maintained under natural photoperiod and ambient temperature. Eight of the mares were treated with domperidone each day from 15 January until the first ovulation of the year. The mean number and size of follicles > or =20 mm in diameter were significantly greater in domperidone-treated mares than in control mares by day 14 of treatment. The day of first ovulation was significantly earlier in domperidone-treated mares than in control mares (mean +/- SEM: 51 +/- 8.2 and 129 +/- 13.6 days, respectively; P < 0.01). The eight domperidone-treated mares all ovulated during treatment and the mean interval from the start of treatment to the day of first ovulation was 27 days (range 15-55 days). Six of the eight domperidone-treated mares underwent normal cycles after the first ovulation, whereas there was a prolonged interval (mean = 67 days) before the second ovulation in the other two mares. Domperidone administration resulted in significantly higher plasma prolactin concentrations measured at 0, 4, 8, 12 and 24 h on day 7 of treatment compared with untreated controls (25.5 +/- 15.8 versus 2.5 +/- 3.0 ng ml(-1)). The concentrations of LH and oestrogen conjugates were significantly higher in domperidone-treated mares compared with control mares by day 28 of treatment. There were no differences in FSH concentrations in domperidone-treated and control mares. The significant increases in follicular development and concentrations of oestrogen conjugates that were observed to occur without concurrent increases in FSH concentration indicate that either domperidone or prolactin may have a direct effect on the ovary.


Subject(s)
Domperidone/pharmacology , Estrous Cycle/drug effects , Estrous Cycle/physiology , Horses/physiology , Seasons , Animals , Dopamine Antagonists/pharmacology , Estrogens/blood , Estrous Cycle/blood , Female , Follicle Stimulating Hormone/blood , Horses/blood , Luteinizing Hormone/blood , Prolactin/blood , Time Factors
8.
South Med J ; 90(2): 249-50, 1997 Feb.
Article in English | MEDLINE | ID: mdl-9042185

ABSTRACT

We present a case of fulminant ulcerative colitis with associated extraintestinal manifestations, including upper airway obstruction from acute laryngotracheitis. Review of the literature shows few other cases of airway obstruction in chronic inflammatory bowel disease. Aggressive medical management of the underlying inflammatory bowel disease may facilitate a conservative approach to airway management, as in this case.


Subject(s)
Airway Obstruction/complications , Colitis, Ulcerative/complications , Adult , Airway Obstruction/drug therapy , Aminosalicylic Acids/therapeutic use , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Colitis, Ulcerative/physiopathology , Female , Humans , Mesalamine
9.
Head Neck ; 18(5): 465-8, 1996.
Article in English | MEDLINE | ID: mdl-8864739

ABSTRACT

BACKGROUND: Fibroinflammatory pseudotumors infrequently involve the major salivary glands. Much more is known regarding the clinical behavior of orbital pseudotumors, especially their steroid responsiveness. METHODS: A case of fibroinflammatory pseudotumor involving the submandibular gland is presented with its preoperative cytologic and computed tomographic scan features. The lesion had a dramatic response to steroids for residual disease. CONCLUSIONS: If preoperative cytologic features of benign spindle cells with an inflammatory component seen on fine-needle aspiration raise the possibility of fibroinflammatory pseudotumor, then responsiveness to high-dose steroids may preclude the need for more-extensive surgery.


Subject(s)
Submandibular Gland Diseases/pathology , Biopsy, Needle , Diagnosis, Differential , Female , Fibrosis , Humans , Middle Aged , Steroids/therapeutic use , Submandibular Gland Diseases/diagnosis , Submandibular Gland Diseases/diagnostic imaging , Submandibular Gland Diseases/drug therapy , Tomography, X-Ray Computed
11.
J Clin Periodontol ; 22(7): 569-77, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7560241

ABSTRACT

The aims of this study were to compare the prevalence of suspected periodontal pathogens in subgingival plaque from 29 HIV seropositive and 27 control subjects and to determine the association of these bacteria with periodontal destruction. Subgingival plaque was collected from the mesiobuccal sites of all teeth, except 3rd molars. Bacteria were identified and enumerated using non-isotopic whole chromosomal DNA probes and a colony lift method. At baseline, HIV seropositive subjects had significantly higher mean % of Porphyromonas gingivalis than control subjects. This difference could be attributed to a subgroup of HIV seropositive subjects with widespread attachment loss. No correlations were observed between the mean %s of DNA probe species and mean attachment loss, CD4 and CD8 T lymphocyte counts or CD4: CD8 ratio. No significant microbiological differences were detected between active and control sites in HIV seropositive subjects on a longitudinal basis. There appeared to be an inverse relationship between the mean %s of P. gingivalis and V. parvula, with respect to progression of HIV infection. The ability of microbiological parameters to predict site-specific breakdown in HIV seropositive subjects requires further investigation.


Subject(s)
HIV Seropositivity/complications , Periodontal Diseases/complications , Bacteria/classification , Bacteria/genetics , Bacteria/isolation & purification , CD4 Lymphocyte Count , CD4-CD8 Ratio , CD8-Positive T-Lymphocytes/pathology , Colony Count, Microbial , DNA Probes , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Dental Plaque/microbiology , Disease Progression , Forecasting , HIV Seronegativity , HIV Seropositivity/immunology , HIV Seropositivity/microbiology , HIV Seropositivity/physiopathology , Humans , Longitudinal Studies , Lymphocyte Count , Periodontal Attachment Loss/complications , Periodontal Attachment Loss/immunology , Periodontal Attachment Loss/microbiology , Periodontal Diseases/immunology , Periodontal Diseases/microbiology , Periodontal Diseases/physiopathology , Porphyromonas gingivalis/isolation & purification , Prevalence , Veillonella/isolation & purification
12.
J Clin Periodontol ; 22(7): 558-68, 1995 Jul.
Article in English | MEDLINE | ID: mdl-7560240

ABSTRACT

The aims of this study were to investigate the prevalence and severity of periodontal diseases in HIV seropositive and assumed HIV seronegative individuals in the Edinburgh area. 29 HIV seropositive subjects were examined at baseline and at 3-monthly intervals. Attachment loss and dichotomous indicators of periodontal disease were recorded. 27 control subjects were seen at baseline only. Although there was no significant difference between the 2 groups in terms of mean attachment loss at baseline, HIV seropositive subjects had experienced more severe attachment loss localised to the lower incisor region. HIV seropositive subjects had a significantly higher mean % of sites exhibiting suppuration and redness. A distinct subgroup of 9 HIV seropositive subjects with widespread attachment loss was detected, which included those subjects with severely affected lower incisors. Site-specific attachment loss of > or = 3 mm was observed in only 20 sites out of 2814 sites in HIV+subjects observed longitudinally. The incidence of aggressive periodontal disease in this study was lower than anticipated, despite the inclusion of patients with advanced HIV infection or AIDS.


Subject(s)
HIV Seropositivity/complications , Periodontal Diseases/complications , Acquired Immunodeficiency Syndrome/complications , Acquired Immunodeficiency Syndrome/pathology , Adult , Case-Control Studies , Cross-Sectional Studies , Disease Progression , Female , Follow-Up Studies , Forecasting , HIV Infections/complications , HIV Infections/pathology , HIV Seronegativity , HIV Seropositivity/pathology , Humans , Incidence , Incisor , Longitudinal Studies , Male , Periodontal Attachment Loss/complications , Periodontal Attachment Loss/pathology , Periodontal Diseases/pathology , Periodontal Pocket/complications , Periodontal Pocket/pathology , Prevalence , Scotland
13.
J Anim Sci ; 73(3): 899-908, 1995 Mar.
Article in English | MEDLINE | ID: mdl-7608025

ABSTRACT

Gravid mares grazing endophyte-infested (E+) tall fescue exhibit increased gestation lengths, agalactia, foal and mare mortality, tough and thickened placentas, weak and dysmature foals, increased sweating during warm weather, reduced serum prolactin and progesterone, and increased serum estradiol-17 beta levels. Also, E+ tall fescue hay is less digestible than endophyte-free (E-) hay. Unlike many other species, horses consuming E+ tall fescue do not exhibit increased body temperature. Young horses consuming only E+ pasture do not gain as well as those consuming E- pasture. There is little difference in gain when the pasture is supplemented with enough concentrate to meet NRC requirements for growth. Neither selenium injections nor supplementing with corn at 50% of the NRC requirements for energy reduces the effects of toxic tall fescue on reproduction and lactation in gravid mares. It seems that the alkaloids of E+ tall fescue are serving as D2 dopamine receptor agonists. This activity would explain their prolactin-lowering effect. Domperidone, a dopamine receptor antagonist, is effective in preventing the signs of tall fescue toxicosis in horses without neuroleptic side effects.


Subject(s)
Animal Feed/poisoning , Ergotism/veterinary , Horse Diseases/physiopathology , Poaceae/microbiology , Pregnancy Complications/veterinary , Acremonium/isolation & purification , Acremonium/metabolism , Animals , Body Temperature/physiology , Domperidone/therapeutic use , Ergot Alkaloids/metabolism , Ergotism/physiopathology , Ergotism/therapy , Estradiol/blood , Female , Horse Diseases/therapy , Horses , Pregnancy , Pregnancy Complications/physiopathology , Pregnancy Complications/therapy , Progesterone/blood , Prolactin/blood , Weight Gain/physiology
14.
Am J Vet Res ; 55(5): 716-21, 1994 May.
Article in English | MEDLINE | ID: mdl-8067623

ABSTRACT

Cultured rat pituitary cells were studied to: determine the effects of ergovaline and loline on in vitro prolactin release; delineate the agonistic activity of these alkaloids at the D2 dopamine receptor, using 2 selective D2 dopamine receptor antagonists; and compare the efficacy of 2 dopamine receptor antagonists in reversing effects of the treatments on in vitro prolactin secretion. Ergovaline reduced in vitro prolactin release by at least 40% (P < 0.05) at concentrations of 10(-4), 10(-6), and 10(-8) M. However, loline reduced (P < 0.05) prolactin release only at the highest concentration, 10(-4) M. Two standard dopamine agonists, dopamine and alpha-ergocryptine, were used to verify that the inhibitory control mechanisms of in vitro prolactin release were intact. Both compounds reduced prolactin release by at least 40% for concentrations of 10(-4), 10(-6), or 10(-8) M. Selective D2 dopamine receptor antagonists (10(-6) M), domperidone and sulpiride, reversed (P < 0.05) the effect of loline on in vitro prolactin release. However, only domperidone (10(-6) M) was able to reverse (P < 0.05) the effect of ergovaline and only at the lowest ergovaline concentration (10(-8) M). Domperidone was more effective (P < 0.05) in reversing the prolactin-suppressing effect of alpha-ergocryptine than was sulpiride. The dose-response curve for domperidone (cubic fit, P < 0.0001) indicated a threshold concentration (10(-7) M) for reversal of alpha-ergocryptine's (10(-8) M) effect on prolactin release. However, at similar concentration of sulpiride (quadratic fit, P < 0.007), a threshold level was not obtained.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Alkaloids/pharmacology , Domperidone/pharmacology , Dopamine/pharmacology , Ergotamines/pharmacology , Pituitary Gland/metabolism , Prolactin/metabolism , Sulpiride/pharmacology , Animals , Cells, Cultured , Dopamine Antagonists , Dose-Response Relationship, Drug , Ergolines/pharmacology , Male , Pituitary Gland/drug effects , Radioimmunoassay , Rats , Rats, Wistar , Vasoconstrictor Agents/pharmacology
15.
Am J Vet Res ; 55(5): 722-9, 1994 May.
Article in English | MEDLINE | ID: mdl-8067624

ABSTRACT

We evaluated the effectiveness of 2 dopamine antagonists as treatments for fescue toxicosis in horses. Sixteen gravid mares were assigned by breed and expected foaling date to 1 of 3 treatment groups: endophyte-infested control; 1.1 mg of domperidone/kg of body weight/d; and 3.3 mg of sulpiride/kg/d. Mares were pastured on endophyte-infected fescue and received 0.454 kg of a corn and dried molasses carrier containing the drug treatment. Treatment started 30 days prior to expected foaling date and continued until parturition. Blood samples were collected, and mammary gland scores were recorded every 5 days. Body weight and body condition scores were obtained every 28 days. Serum was analyzed for prolactin, progesterone, and estradiol-17 beta concentrations. Domperidone-treated mares had shorter (P = 0.09) gestation duration and foaled closer (P = 0.07) to their expected parturition date than did control mares. Mammary gland scores were higher (P < 0.05) for domperidone-treated mares than for control mares. By 4 and 9 days after the start of treatment, serum prolactin concentration was higher (P < 0.05) in domperidone-treated mares and sulpiride-treated mares, respectively, than in control mares. Domperidone- and sulpiride-treated mares had higher (P < 0.05) serum progesterone and lower (P < 0.01) estradiol-17 beta concentrations than did control mares. These results indicate that domperidone may offer considerable potential as a treatment for fescue toxicosis in horses.


Subject(s)
Animal Feed , Domperidone/therapeutic use , Foodborne Diseases/veterinary , Horse Diseases , Placenta/physiopathology , Poaceae , Pregnancy Complications/veterinary , Sulpiride/therapeutic use , Animals , Animals, Newborn , Body Weight , Estradiol/blood , Female , Foodborne Diseases/drug therapy , Foodborne Diseases/physiopathology , Gestational Age , Horses , Lactation/drug effects , Placenta/drug effects , Placenta/physiology , Pregnancy , Pregnancy Complications/drug therapy , Pregnancy Complications/physiopathology , Progesterone/blood , Prolactin/blood
16.
J Anim Sci ; 71(12): 3199-205, 1993 Dec.
Article in English | MEDLINE | ID: mdl-8294270

ABSTRACT

During a 3-yr period 184 steers were allotted to five treatment groups. Group 1 steers were pastured on fescue-clover and slaughtered in the spring at approximately 540 d of age. Group 2 steers were pastured on fescue-clover then placed on summer pasture regimens and slaughtered at approximately 620 d of age. Group 3 steers were treated the same as Group 2 then placed in the drylot for 45 d. Group 4 steers were treated the same as Group 2 steers then placed in the drylot for 75 d. Group 5 steers were placed in drylot after weaning and conditioning and were slaughtered at endpoints that corresponded with those for Group 3. Steers selected for slaughter at each endpoint were those evaluated to have reached the most optimum slaughter weight and finish by project personnel. Carcasses of steers from fescue-clover and summer pasture had lower yield grades and a lower quality grade than carcasses of steers from drylot (P < .05). Carcasses of steers from fescue-clover and summer pasture had a more yellow fat covering than carcasses of steers that received a concentrate (Treatments 3, 4, and 5, P < .0001). Fescue-clover- and summer pasture-fed steers had a higher percentage of lean and a lower percentage of fat in the 9-10-11th rib section than did steers that received concentrate (P < .005). Chemical analysis of the lean tissue of the 9-10-11th rib section indicated that summer pasture-fed steers had a lower percentage of ether extract and higher percentage of moisture than the lean tissue from steers that received concentrate (P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Animal Feed , Cattle/growth & development , Meat/standards , Adipose Tissue/growth & development , Animals , Fabaceae , Male , Meat/analysis , Muscle Development , Plants, Medicinal , Poaceae , Silage , Taste
17.
Arch Oral Biol ; 38(11): 931-5, 1993 Nov.
Article in English | MEDLINE | ID: mdl-7507660

ABSTRACT

This paper reports the development of a protocol allowing hybridization and detection of DNA fixed to nylon colony lifts from up to three species of bacteria simultaneously. Half ml samples of serial dilutions of pure cooked-meat broth (CMB) cultures of Capnocytophaga ochracea, Actinobacillus actinomycetemcomitans and Prevotella intermedia were grown on trypticase soy blood agar (TSBA) plates for 7 days in an anaerobic chamber. From the same CMBs a further set of dilutions was completed that contained all three species. Samples from these dilutions produced mixed-growth TSBA plates following anaerobic incubation for 7 days. After incubation, colony counts on pure-growth TSBA plates were enumerated by colony counter. Colony counts of C. ochracea, A. actinomycetemcomitans and P. intermedia on mixed-growth TSBA plates were enumerated by nylon colony lift, simultaneous hybridization with non-isotopic whole chromosomal DNA probes and alkaline phosphatase substrates generating three colours. The results indicate that the protocol correctly identified and differentiated between the three species on mixed-growth TSBA plates. The proportions of each species and mean total colony count expected by counting pure plates were in agreement with the proportions of each species and total colony counts enumerated by DNA probes on mixed growth plates. The development of simultaneous hybridization and multicolour detection will result in improved data recovery from dental plaque samples, in addition to reducing the cost and labour required in current colony-lift protocols, without affecting the specificity or sensitivity of the probes used.


Subject(s)
Bacteria, Anaerobic/isolation & purification , DNA Probes , DNA, Bacterial/analysis , Dental Plaque/microbiology , In Situ Hybridization/methods , Aggregatibacter actinomycetemcomitans/genetics , Aggregatibacter actinomycetemcomitans/isolation & purification , Alkaline Phosphatase , Bacteria, Anaerobic/genetics , Bacteroides/genetics , Bacteroides/isolation & purification , Biotin , Capnocytophaga/genetics , Capnocytophaga/isolation & purification , Colony Count, Microbial , Colorimetry , Digoxigenin , Fluoresceins , Histocytochemistry , Humans , Staining and Labeling , Substrate Specificity
18.
Vet Hum Toxicol ; 35(5): 454-64, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8249272

ABSTRACT

Studies of fescue toxicosis have identified several classes of plant/fungal alkaloids that may be responsible for the toxicosis problem of endophyte (Acremonium coenophialum) infected tall fescue (Festuca arundinacea). This review explores the interaction of these alkaloids with the animal's system and attempts to explain the various anomalies of fescue toxicosis through discussion of both in vitro and in vivo experiments.


Subject(s)
Acremonium , Cattle Diseases/chemically induced , Mycotoxins/poisoning , Plant Poisoning/veterinary , Poaceae/microbiology , Agriculture/economics , Animals , Cattle , Cattle Diseases/blood , Mycotoxins/chemistry , Mycotoxins/economics , Plant Poisoning/blood , Plant Poisoning/complications , Plant Poisoning/etiology , Prolactin/blood , Receptors, Dopamine/analysis
19.
J Anim Sci ; 70(9): 2779-86, 1992 Sep.
Article in English | MEDLINE | ID: mdl-1399895

ABSTRACT

The objective of this research was to measure the effects of endophyte-infected tall fescue seed extract and various alkaloids associated with the endophyte on in vitro prolactin secretion by rat hemipituitaries. Rat anterior pituitaries (AP) were dissected into halves and placed in temperature-controlled culture chambers (37 degrees C). The tissue was perfused with culture media at a flow rate of 12 mL/h. After perfusion for at least 90 min with control media, AP halves were exposed to their respective treatments for 15 min before they were returned to the control media. The treatments for Exp. 1 were .01 micrograms of alpha-ergocryptine/mL of culture medium, .01 microgram of ergonovine/mL of culture medium, .01 gram-equivalents of endophyte-infected tall fescue seed/mL of culture medium, and .01 gram-equivalents of endophyte free tall fescue seed/mL of culture medium. Treatments for Exp. 2 consisted of 10(-4), 10(-6), and 10(-8) M concentrations of perloline, N-formyl loline, N-acetyl loline, N-methyl loline, and alpha-ergocryptine. alpha-Ergocryptine suppressed (P less than .10) prolactin secretion in both experiments. Ergonovine and perloline both stimulated (P less than .10) prolactin secretion. The loline alkaloids (N-formyl loline, N-acetyl loline, N-methyl loline) had no effect on prolactin secretion. The endophyte-infected seed extract treatment suppressed (P less than .10) prolactin secretion. The endophyte-free seed extract treatment had no effect on prolactin secretion. In Exp. 2, prolactin secretion from AP responded to alpha-ergocryptine treatment in a dose-dependent fashion.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Acremonium/growth & development , Alkaloids/toxicity , Phenanthrenes , Pituitary Gland, Anterior/drug effects , Poaceae/microbiology , Prolactin/metabolism , Animals , Dose-Response Relationship, Drug , Ergolines/toxicity , Ergonovine/toxicity , Male , Organ Culture Techniques , Pituitary Gland, Anterior/metabolism , Plant Extracts/toxicity , Rats , Rats, Sprague-Dawley , Seeds
20.
Theriogenology ; 38(3): 375-84, 1992 Sep.
Article in English | MEDLINE | ID: mdl-16727141

ABSTRACT

In an experiment with a 2x2 factorial arrangement of treatments, 32 virgin Angus heifers were used to study effects of tall fescue endophyte Acremonium coenophialum and energy supplementation on reproductive performance. Treatments were feeding of endophyte-free (EF) or endophyte-infected (EI) tall fescue without (0%) and with energy supplementation at one percent group mean body weight (1%). Blood samples were collected at 28-day intervals for prolactin (PRL). Starting 21 days before the breeding season, blood was also collected weekly for progesterone (P(4)) and PRL. After 99 days of grazing, heifers received Syncro-Mate-B (SMB) and were then artificially inseminated over a 30-day period. Heifers on EI-0% had a lower (P<0.05) average final body weight. Highest average daily gain was observed in heifers fed EF-1% (0.98 kg/day), whereas, heifers fed EI-0% had lowest average daily gain (0.53 kg/day; P<0.05). Endophyte-infected fescue depressed serum PRL concentrations (171 and 143 ng/ml vs 70 and 85 ng/ml; EF-0% and EF-1% vs EI-0% and EI-1%, respectively; P<0.05). No differences were observed among treatments in luteal activity before or after SMB, in pregnancy rates after a synchronized estrus or in overall pregnancy rates during the 30-day breeding season. Endophyte-infected fescue decreased average daily gain and depressed serum concentrations of prolactin, but had no effect on reproductive performance as measured in this study.

SELECTION OF CITATIONS
SEARCH DETAIL
...