Cold-related Florida manatee mortality in relation to air and water temperatures.

Many tropical and subtropical species are sensitive to sudden temperature changes, especially drops in temperature. During winters 2009-2010 and 2010-2011, unusually cold temperatures occurred in many parts of Florida, USA, resulting in increased mortality of Florida manatees, sea turtles, fish, corals, and other species. The Florida manatee, in particular, is highly susceptible to cold stress and death when water temperatures drop below 20°C. We sought to characterize the magnitude and timing of reports of cold-related manatee carcasses in relation to fluctuations in water and air temperatures in central-east and central-west Florida during the six winters from 2008 to 2014. We used a generalized linear model to predict counts of manatee carcasses with a cold-related cause of death reported over 7-day bins in relation to various short-term (two weeks or less) and cumulative (incrementally summed from the start of the winter) heating-degree-day effects (HDD; < 20°C) and a categorical winter variable. Using water temperature data, the top-ranked model in both regions included a short-term temperature effect (14-day HDD sum) that preceded increases in reports of cold-related manatee carcasses by 7 days. Cumulative exposure to cold weather over the winter amplified effects on mortality in the central-east region. Quantifying the relationship between cold events and manatee mortality helps us prepare for rescue and salvage operations when extremely cold weather is forecast. This is especially important because anticipated loss or degradation of warm-water refuges due to human activities and sea level rise could potentially impact the manatee population in the future. These methods could also be applied to other species susceptible to cold-related mortality.

Prevalence of avian paramyxovirus 1 and avian influenza virus in double-crested Cormorants (Phalacrocorax auritus) in eastern North America.

Although it is well established that wild birds, such as cormorants, carry virulent avian paramyxovirus serotype 1 (APMV-1; causative agent of Newcastle disease) and avian influenza virus (AIV), the prevalence of these viruses among Double-crested Cormorants (Phalacrocorax auritus) in the Great Lakes region of North America has not been rigorously studied. We determined the prevalences of APMV-1 and AIV in Double-crested Cormorants from the interior population of eastern North America. From 2009 to 2011, oropharyngeal and cloacal swabs and serum samples were collected from 1,957 individual Double-crested Cormorants, ranging from chicks to breeding adults, on breeding colony sites in Michigan, Wisconsin, and Mississippi, USA, and Ontario, Canada, as well as on the wintering grounds of migratory populations in Mississippi, USA. Prevalence of antibodies to APMV-1 in after-hatch year birds was consistently high across all three years, ranging from 86.3% to 91.6%. Antibody prevalences in chicks were much lower: 1.7, 15.3, and 16.4% in 2009, 2010, and 2011, respectively. Virulent APMV-1 was detected in six chicks sampled in 2010 in Ontario, Canada. Only one adult was positive for AIV-specific antibodies and five individuals were positive for AIV matrix protein, but the latter were negative for H5 and H7 AIV subtypes. We provide further evidence that Double-crested Cormorants play an important role in the maintenance and circulation of APMV-1 in the wild, but are unlikely to be involved in the circulation of AIV.