Subject(s)
Inflammation/etiology , Lymphatic System/radiation effects , Microcirculation/radiation effects , Radiation Injuries, Experimental/pathology , Age Factors , Animals , Biogenic Amines , Carrageenan , Dose-Response Relationship, Radiation , Erythema/etiology , Inflammation/chemically induced , Rats , p-Methoxy-N-methylphenethylamineABSTRACT
Subdermal inoculation of the foot of the rat with lethally irradiated (LI) Walker tumour (W256) cells, mixed with viable (V) W256 cells, decreased the latent period for initiation of allogeneic tumour growth without significantly affecting its rate. This Révész effect decreased with increase in the number of inoculated V cells, and with decrease in age of recipient. LI cells of a different (Y-P388) rat tumour exerted a Révész effect, even in recipients which had been immunized with LI (Y-P388) tumour cells. Local pre-irradiation of the site of inoculation of V cells decreased both the latent period and rate of tumour growth. It acted independently of a Révész effect, and the decrease in tumour growth rate was partly due to emigration of V cells from the inoculum, producing metastases. LI, but not heat-killed cells, induced prolonged swelling of the tumour bed in unimmunized and tumour-immunized rats, which, unlike inflammatory swelling, was inhibited by pre-irradiation of the foot. It is postulated that the Révész effect is due to enhancement of survival of V cells by trophic substances which are principally elaborated by LI (AND V) cells, but also by the tumour bed, due to innate growth and trophic reactions of its tissues to the presence of tumour cells.
Subject(s)
Carcinoma 256, Walker/immunology , Immunosuppression Therapy , Radiation Effects , Age Factors , Animals , Carcinoma 256, Walker/metabolism , Carcinoma 256, Walker/pathology , Neoplasm Transplantation , Rats , Sarcoma, Yoshida/immunology , Time Factors , Transplantation, HomologousABSTRACT
An apparatus which has been widely used in rats for measuring swelling of the foot induced locally by inflammatory agents has been adapted to measure rapidly, accurately and objectively, the growth of tumour cells transplanted to the foot, and the reactions of the normal tissues (tumour bed) to tumour growth. General features on the apparatus and the techniques used are described. Examples are provided of preliminary measurements made of normal growth of the foot, reactions of the foot to two injurious agents (histamine and Corynebacterium parvum) and of growth of allogeneic (W256) tumour cells.
